Vaccines, Vol. 8, Pages 10: Investigating Prime-Pull Vaccination through a Combination of Parenteral Vaccination and Intranasal Boosting:
Vaccines, Vol. 8, Pages 10: Investigating Prime-Pull Vaccination through a Combination of Parenteral Vaccination and Intranasal Boosting
Vaccines doi: 10.3390/vaccines8010010
Authors:
Carla B. Roces
Maryam T. Hussain
Signe T. Schmidt
Dennis Christensen
Yvonne Perrie
Formulation of inhalable delivery systems containing tuberculosis (TB) antigens to target the site of infection (lungs) have been considered for the development of subunit vaccines. Inert delivery systems such as poly (lactic-co-glycolic acid) (PLGA) are an interesting approach due to its approval for human use. However, PLGA suffers hydrolytic degradation when stored in a liquid environment for prolonged time. Therefore, in this study, nano- and microparticles composed of different PLGA copolymers (50:50, 75:25 and 85:15), sucrose (10% w/v) and L-leucine (1% w/v) encapsulating H56 TB vaccine candidate were produced as dried powders. In vitro studies in three macrophage cell lines (MH-S, RAW264.7 and THP-1) showed the ability of these cells to take up the formulated PLGA:H56 particles and process the antigen. An in vivo prime-pull immunisation approach consisting of priming with CAF01:H56 (2 × subcutaneous (s.c.) injection) followed by a mucosal boost with PLGA:H56 (intranasal (i.n.) administration) demonstrated the retention of the immunogenicity of the antigen encapsulated within the lyophilised PLGA delivery system, although no enhancing effect could be observed compared to the administration of antigen alone as a boost. The work here could provide the foundations for the scale independent manufacture of polymer delivery systems encapsulating antigens for inhalation/aerolisation to the lungs.
Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,00306932607174,alsfakia@gmail.com,
Πληροφορίες
Ετικέτες
Δευτέρα 30 Δεκεμβρίου 2019
Investigating Prime-Pull Vaccination through a Combination of Parenteral Vaccination and Intranasal Boosting
Αναρτήθηκε από
Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,00306932607174,alsfakia@gmail.com,
στις
11:38 μ.μ.
Ετικέτες
00302841026182,
00306932607174,
alsfakia@gmail.com,
Anapafseos 5 Agios Nikolaos 72100 Crete Greece,
Medicine by Alexandros G. Sfakianakis,
Telephone consultation 11855 int 1193
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
Αρχειοθήκη ιστολογίου
-
►
2023
(276)
- ► Φεβρουαρίου (133)
- ► Ιανουαρίου (143)
-
►
2022
(1976)
- ► Δεκεμβρίου (116)
- ► Σεπτεμβρίου (158)
- ► Φεβρουαρίου (165)
- ► Ιανουαρίου (161)
-
►
2021
(3661)
- ► Δεκεμβρίου (161)
- ► Σεπτεμβρίου (274)
- ► Φεβρουαρίου (64)
- ► Ιανουαρίου (368)
-
►
2020
(4554)
- ► Δεκεμβρίου (400)
- ► Σεπτεμβρίου (381)
- ► Φεβρουαρίου (638)
- ► Ιανουαρίου (691)
-
▼
2019
(4999)
-
▼
Δεκεμβρίου
(924)
- Happy New Year ! God is our strength. Let Him be t...
- Inability to close mouth and dysphagia caused by p...
- Piperacillin-tazobactam induced bicytopenia in low...
- Acute cerebellar ataxia due to Epstein-Barr virus ...
- Pericardial knock
- Paternal uniparental disomy for chromosome 14: pre...
- Chronic mesenteric ischaemia masked by candida eso...
- History, treatment and analysis of a rare form of ...
- History, treatment and analysis of a rare form of ...
- Extracellular Vesicles from Mesenchymal Stem Cells...
- Signaling Network of Forkhead Family of Transcript...
- Csf1 Deficiency Dysregulates Glial Responses to De...
- Spontaneous Ultraslow Na+ Fluctuations in the Neon...
- Journal of Voice
- Thorough performance evaluation of 213 nm ultravio...
- The Xylella fastidiosa-Resistant Olive Cultivar “L...
- GEO series published today
- Comparative Pharmacokinetic Study of Taxifolin aft...
- Investigating Prime-Pull Vaccination through a Com...
- New GEO Series
- Transcription profiling of dendritic cells differe...
- Chemotherapeutic remodeling of the gut microbiome ...
- DNA methylation and hydroxymethylation mediated ge...
- The histone methyltransferase SETDB1 contributes t...
- HIF1α/HIF2α-miR210-3p network promotes glioblastom...
- H3K27Ac ChIP-seq on Patient Dirived Xenograft (PDX...
- CRISPR/Cas9-mediated gene correction in newborn ra...
- Genome-wide localisation of histone H3S57ph in pro...
- Pathway level gating of auxin signalling provides ...
- Single cell transcriptome sequencing of gastric ca...
- Whole kidneys at embryonic day 16.5: wildtype (WT)...
- Antifungal Activity of Beauveria bassiana Endophyt...
- Immune Responses after Vascular Photodynamic Thera...
- One Anastomosis Gastric Bypass Reconstitutes the A...
- Immune Checkpoint Inhibitor Rechallenge in Patient...
- Ontogenetic Pattern Changes of Nucleobindin-2/Nesf...
- Latent Tuberculosis Infection Treatment Completion...
- The Chromatin Remodelling Contributions of Snf2l i...
- CD271/p75NTR is a novel diagnostic marker, prognos...
- Chemogenomic profiling of breast cancer patient-de...
- Sequencing of canine and genetically-engineered mo...
- Telomeres suppress the activity of retrotransposon...
- Bu Shen Zhu Yun Decoction Improves Endometrial Rec...
- Multicentre, non-interventional study of the effic...
- Finding/identifying primaries with neck disease (F...
- Intermittent theta burst stimulation applied durin...
- Multidimensional impact of severe mental illness o...
- Clinical evaluation of percutaneous transforaminal...
- Digital phenotyping for assessment and prediction ...
- Research priorities in children requiring elective...
- Complications after surgery for benign prostatic e...
- Identifying patient concerns during consultations ...
- Better before-better after: efficacy of prehabilit...
- Elderly migrants : Effect of social integration on...
- Pattern and probability of dispensing of prescript...
- Improving mental health and physiological stress r...
- B!RTH: a mixed-methods survey of audience members ...
- Vitamin D in the prevention of exacerbations of as...
- Advanced practice nurses, registered nurses and me...
- Complement activation in individuals with previous...
- Thyroid Hormone Receptor β Inhibits Self-Renewal C...
- Macrofollicular Variant of Follicular Thyroid Carc...
- Trained immunity confers broad-spectrum protection...
- IGFBP7 acts as a negative regulator of RANKL‐induc...
- Subgingival Instrumentation for Treatment of Perio...
- The Spine Functional Index
- Sarcomatoid Dedifferentiation in Renal Cell Carcin...
- Negative Impact of Wound Complications on Oncologi...
- Nanoformulated Zoledronic Acid Boosts the Vδ2 T Ce...
- Autophagy in the Immunosuppressive Perivascular Mi...
- Molecular Profiling of Atypical Tenosynovial Giant...
- Biomarkers of meat and seafood intake: an extensiv...
- Non ‐alcoholic steatohepatitis mimicking solitary ...
- Diffusion-Weighted MR Imaging of Primary and Secon...
- Clear cell carcinoma of the anterior abdominal wal...
- Sister Mary Joseph nodule: an often overlooked or ...
- Superb microvascular imaging technique in depictin...
- Protean Manifestations and Diagnostic Challenges I...
- Thyroid Dose Estimates for a Cohort of Belarusian ...
- Dose to Medical Personnel
- Factors Determining Work Arduousness Levels among ...
- Treatment of Miller Class I Gingival Recession wit...
- Vitamin K and Bone Health: A Review on the Effects...
- Laparoscopic Partial Nephrectomy for cT1 Tumors
- A Non-Interventional Pilot Study to Explore the Ro...
- Anlotinib Combined With TACE in Hepatocellular Car...
- Testing the Addition of the Immunotherapy Drug, Pe...
- Management of Cognitive Difficulties After Cancer ...
- Nab-paclitaxel Versus Topotecan As Second-Line Tre...
- Sintilimab Combined With Bevacizumab for Brain Met...
- SHR-1210 Combined With Albumin-bound Paclitaxel an...
- Heated Intra-peritoneal Chemotherapy With Doxorubi...
- Ribociclib and Spartalizumab in R/M HNSCC
- Biomolecules, Vol. 10, Pages 66: The Emerging Role...
- Evaluating the effectiveness of adjuvant radiother...
- Examining seasonal variation in epistaxis in a mar...
- Is the survival rate for acral melanoma actually w...
- The Improved Effects of a Multidisciplinary Team o...
- Treatment of an Adult Skeletal Class III Patient w...
- Significant influencing factors and practical solu...
- ► Σεπτεμβρίου (845)
-
▼
Δεκεμβρίου
(924)
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου