Dynamic proteome profiling of human pluripotent stem cell‐derived pancreatic progenitors

Dynamic proteome profiling of human pluripotent stem cell‐derived pancreatic progenitors:

Proteome adaptations occur in waves during early stages of hPSC differentiation towards pancreatic progenitors. In this study, we utilized quantitative mass spectrometry‐based proteomics, an extremely useful tool, for the discovery of novel molecular signatures of intermediate stages during early pancreatic differentiation. Here, we uncovered a potential regulatory role of the Hippo signaling pathway during hPSC differentiation towards pancreatic progenitors.

Abstract

A comprehensive characterization of the molecular processes controlling cell fate decisions is essential to derive stable progenitors and terminally differentiated cells that are functional from human pluripotent stem cells (hPSCs). Here we report the use of quantitative proteomics to describe early proteome adaptations during hPSC differentiation towards pancreatic progenitors. We report that the use of unbiased quantitative proteomics allows the simultaneous profiling of numerous proteins at multiple time points, and is a valuable tool to guide the discovery of signaling events and molecular signatures underlying cellular differentiation. We also monitored the activity level of pathways whose roles are pivotal in the early pancreas differentiation, including the Hippo signaling pathway. The quantitative proteomics dataset provides insights into the dynamics of the global proteome during the transition of hPSCs from a pluripotent state towards pancreatic differentiation.

© AlphaMed Press 2019

Significance Statement

We present quantitative proteomic profiling of early proteome adaptations during hPSC differentiation towards the pancreatic progenitors. We mapped the activation profiles of known signaling pathways at various stages during early pancreatic differentiation, and discovered signaling events and novel molecular signatures, including a potential regulatory role for the Hippo signaling pathway during early pancreatic differentiation.