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Equine Vet J. 2019 Dec 02;:
Authors: Stokes SM, Bertin FR, Stefanovski D, Poulsen L, Belknap JK, Medina-Torres CE, Pollitt CC, van Eps AW
Abstract
BACKGROUND: Continuous digital hypothermia (CDH) prevents lamellar failure in the euglycaemic hyperinsulinaemic clamp (EHC) and oligofructose (OF) laminitis models, but the mechanisms remain unclear.
OBJECTIVES: To evaluate the effects of CDH on lamellar energy metabolism and perfusion in healthy horses and during EHC and OF laminitis models.
STUDY DESIGN: In vivo experiment.
METHODS: Archived samples were used from Standardbred geldings that received no treatment (CON) (n = 8) or underwent EHC (n = 8) or OF (n = 6) laminitis models. Both forelimbs were instrumented with a lamellar microdialysis system, and one forelimb was cooled (CDH) with the other maintained at ambient temperature (AMB). Microdialysate was collected every 6 h and analysed for glucose, lactate and pyruvate concentrations and lactate to pyruvate ratio (L:P). Microdialysis urea clearance was used to estimate lamellar tissue perfusion. Data were analysed using a mixed-effects linear regression model.
RESULTS: Glucose did not change in CDH limbs relative to AMB in CON (P = 0.3), EHC (P = 0.3) or OF (P = 0.6) groups. There was a decrease in lactate (P<0.001) and pyruvate (P<0.01) in CDH limbs relative to AMB in all groups. L:P decreased in CON CDH relative to CON AMB (P<0.001) but was not different in EHC (P = 0.6) and OF (P = 0.07) groups. Urea clearance decreased in CDH limbs relative to AMB in CON (P = 0.002) and EHC (P<0.001), but not in OF (P = 0.4).
MAIN LIMITATIONS: The EHC model may not mimic natural endocrinopathic laminitis.
CONCLUSIONS: CDH caused a marked decrease in lamellar glucose metabolism (CON, EHC and OF) and perfusion (CON and EHC) without affecting lamellar glucose concentration. Although cellular energy failure is not a primary pathophysiologic event in EHC and OF laminitis models, CDH may act by limiting energy supply to pathologic cellular processes whilst preserving those critical to lamellar homeostasis.
PMID: 31793047 [PubMed - as supplied by publisher]
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