The Potential Role of Cycloastragenol in Promoting Diabetic Wound Repair In Vitro: Background. Refractory wound healing is a severe complication of diabetes with a significant socioeconomic burden. Whereas current therapies are insufficient to accelerate repair, stem cell-based therapy is increasingly recognized as an alternative that improves healing outcomes. The aim of the present study is to explore the role of cycloastragenol (CAG), a naturally occurring compound in Astragali Radix, in ameliorating refractory cutaneous wound healing in vitro, which may provide a new insight into therapeutic strategy for diabetic wounds. Methods. Human epidermal stem cells (EpSCs) obtained from nine patients were exposed to CAG, with or without DKK1 (a Wnt signaling inhibitor). A lentiviral short hairpin RNA (shRNA) system was used to establish the telomerase reverse transcriptase (TERT) and β-catenin knockdown cell line. Cell counting kit-8, scratch wound healing, and transwell migration assay were used to determine the effects of CAG in cell growth and migration. The activation of TERT, β-catenin, and c-Myc was determined using real-time qPCR and western blot analysis. Chromatin immunoprecipitation (ChIP) was performed to evaluate the associations among CAG, TERT, and Wnt/β-catenin signals. Results. CAG not only promoted the proliferation and migration ability of EpSCs but also increased the expression levels of TERT, β-catenin, c-Myc. These effects of CAG were most pronounced at a dose of 0.3 μM. Notably, the CAG-promoted proliferative and migratory abilities of EpSCs were abrogated in TERT and β-catenin-silenced cells. In addition, the ChIP results strongly suggested that CAG-modulated TERT was closely associated with the activation of Wnt/β-catenin signaling. Conclusion. Our data indicate that CAG is a TERT activator of EpSCs and is associated with their proliferation and migration, a role it may play through the activation of Wnt/β-catenin signaling.
Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,00306932607174,alsfakia@gmail.com,
Πληροφορίες
Ετικέτες
Παρασκευή 20 Δεκεμβρίου 2019
The Potential Role of Cycloastragenol in Promoting Diabetic Wound Repair In Vitro
Αναρτήθηκε από
Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,00306932607174,alsfakia@gmail.com,
στις
12:09 π.μ.
Ετικέτες
00302841026182,
00306932607174,
alsfakia@gmail.com,
Anapafseos 5 Agios Nikolaos 72100 Crete Greece,
Medicine by Alexandros G. Sfakianakis,
Telephone consultation 11855 int 1193
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
Αρχειοθήκη ιστολογίου
-
►
2023
(276)
- ► Φεβρουαρίου (133)
- ► Ιανουαρίου (143)
-
►
2022
(1976)
- ► Δεκεμβρίου (116)
- ► Σεπτεμβρίου (158)
- ► Φεβρουαρίου (165)
- ► Ιανουαρίου (161)
-
►
2021
(3661)
- ► Δεκεμβρίου (161)
- ► Σεπτεμβρίου (274)
- ► Φεβρουαρίου (64)
- ► Ιανουαρίου (368)
-
►
2020
(4554)
- ► Δεκεμβρίου (400)
- ► Σεπτεμβρίου (381)
- ► Φεβρουαρίου (638)
- ► Ιανουαρίου (691)
-
▼
2019
(4999)
-
▼
Δεκεμβρίου
(924)
- Happy New Year ! God is our strength. Let Him be t...
- Inability to close mouth and dysphagia caused by p...
- Piperacillin-tazobactam induced bicytopenia in low...
- Acute cerebellar ataxia due to Epstein-Barr virus ...
- Pericardial knock
- Paternal uniparental disomy for chromosome 14: pre...
- Chronic mesenteric ischaemia masked by candida eso...
- History, treatment and analysis of a rare form of ...
- History, treatment and analysis of a rare form of ...
- Extracellular Vesicles from Mesenchymal Stem Cells...
- Signaling Network of Forkhead Family of Transcript...
- Csf1 Deficiency Dysregulates Glial Responses to De...
- Spontaneous Ultraslow Na+ Fluctuations in the Neon...
- Journal of Voice
- Thorough performance evaluation of 213 nm ultravio...
- The Xylella fastidiosa-Resistant Olive Cultivar “L...
- GEO series published today
- Comparative Pharmacokinetic Study of Taxifolin aft...
- Investigating Prime-Pull Vaccination through a Com...
- New GEO Series
- Transcription profiling of dendritic cells differe...
- Chemotherapeutic remodeling of the gut microbiome ...
- DNA methylation and hydroxymethylation mediated ge...
- The histone methyltransferase SETDB1 contributes t...
- HIF1α/HIF2α-miR210-3p network promotes glioblastom...
- H3K27Ac ChIP-seq on Patient Dirived Xenograft (PDX...
- CRISPR/Cas9-mediated gene correction in newborn ra...
- Genome-wide localisation of histone H3S57ph in pro...
- Pathway level gating of auxin signalling provides ...
- Single cell transcriptome sequencing of gastric ca...
- Whole kidneys at embryonic day 16.5: wildtype (WT)...
- Antifungal Activity of Beauveria bassiana Endophyt...
- Immune Responses after Vascular Photodynamic Thera...
- One Anastomosis Gastric Bypass Reconstitutes the A...
- Immune Checkpoint Inhibitor Rechallenge in Patient...
- Ontogenetic Pattern Changes of Nucleobindin-2/Nesf...
- Latent Tuberculosis Infection Treatment Completion...
- The Chromatin Remodelling Contributions of Snf2l i...
- CD271/p75NTR is a novel diagnostic marker, prognos...
- Chemogenomic profiling of breast cancer patient-de...
- Sequencing of canine and genetically-engineered mo...
- Telomeres suppress the activity of retrotransposon...
- Bu Shen Zhu Yun Decoction Improves Endometrial Rec...
- Multicentre, non-interventional study of the effic...
- Finding/identifying primaries with neck disease (F...
- Intermittent theta burst stimulation applied durin...
- Multidimensional impact of severe mental illness o...
- Clinical evaluation of percutaneous transforaminal...
- Digital phenotyping for assessment and prediction ...
- Research priorities in children requiring elective...
- Complications after surgery for benign prostatic e...
- Identifying patient concerns during consultations ...
- Better before-better after: efficacy of prehabilit...
- Elderly migrants : Effect of social integration on...
- Pattern and probability of dispensing of prescript...
- Improving mental health and physiological stress r...
- B!RTH: a mixed-methods survey of audience members ...
- Vitamin D in the prevention of exacerbations of as...
- Advanced practice nurses, registered nurses and me...
- Complement activation in individuals with previous...
- Thyroid Hormone Receptor β Inhibits Self-Renewal C...
- Macrofollicular Variant of Follicular Thyroid Carc...
- Trained immunity confers broad-spectrum protection...
- IGFBP7 acts as a negative regulator of RANKL‐induc...
- Subgingival Instrumentation for Treatment of Perio...
- The Spine Functional Index
- Sarcomatoid Dedifferentiation in Renal Cell Carcin...
- Negative Impact of Wound Complications on Oncologi...
- Nanoformulated Zoledronic Acid Boosts the Vδ2 T Ce...
- Autophagy in the Immunosuppressive Perivascular Mi...
- Molecular Profiling of Atypical Tenosynovial Giant...
- Biomarkers of meat and seafood intake: an extensiv...
- Non ‐alcoholic steatohepatitis mimicking solitary ...
- Diffusion-Weighted MR Imaging of Primary and Secon...
- Clear cell carcinoma of the anterior abdominal wal...
- Sister Mary Joseph nodule: an often overlooked or ...
- Superb microvascular imaging technique in depictin...
- Protean Manifestations and Diagnostic Challenges I...
- Thyroid Dose Estimates for a Cohort of Belarusian ...
- Dose to Medical Personnel
- Factors Determining Work Arduousness Levels among ...
- Treatment of Miller Class I Gingival Recession wit...
- Vitamin K and Bone Health: A Review on the Effects...
- Laparoscopic Partial Nephrectomy for cT1 Tumors
- A Non-Interventional Pilot Study to Explore the Ro...
- Anlotinib Combined With TACE in Hepatocellular Car...
- Testing the Addition of the Immunotherapy Drug, Pe...
- Management of Cognitive Difficulties After Cancer ...
- Nab-paclitaxel Versus Topotecan As Second-Line Tre...
- Sintilimab Combined With Bevacizumab for Brain Met...
- SHR-1210 Combined With Albumin-bound Paclitaxel an...
- Heated Intra-peritoneal Chemotherapy With Doxorubi...
- Ribociclib and Spartalizumab in R/M HNSCC
- Biomolecules, Vol. 10, Pages 66: The Emerging Role...
- Evaluating the effectiveness of adjuvant radiother...
- Examining seasonal variation in epistaxis in a mar...
- Is the survival rate for acral melanoma actually w...
- The Improved Effects of a Multidisciplinary Team o...
- Treatment of an Adult Skeletal Class III Patient w...
- Significant influencing factors and practical solu...
- ► Σεπτεμβρίου (845)
-
▼
Δεκεμβρίου
(924)
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου