Study of the Expression Transition of Cardiac Myosin Using Polarization-Dependent SHG Microscopy.

Study of the Expression Transition of Cardiac Myosin Using Polarization-Dependent SHG Microscopy.:

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Study of the Expression Transition of Cardiac Myosin Using Polarization-Dependent SHG Microscopy.

Biophys J. 2020 Jan 03;:

Authors: Yuan C, Zhao X, Wang Z, Borg TK, Ye T, Khalpey ZI, Runyan RB, Shao Y, Gao BZ

Abstract

Detection of the transition between the two myosin isoforms α- and β-myosin in living cardiomyocytes is essential for understanding cardiac physiology and pathology. In this study, the differences in symmetry of polarization spectra obtained from α- and β-myosin in various mammalian ventricles and propylthiouracil-treated rats are explored through polarization-dependent second harmonic generation microscopy. Here, we report for the, to our knowledge, first time that α- and β-myosin, as protein crystals, possess different symmetries: the former has C6 symmetry, and the latter has C3v. A single-sarcomere line scan further demonstrated that the differences in polarization-spectrum symmetry between α- and β-myosin came from their head regions: the head and neck domains of α- and β-myosin account for the differences in symmetry. In addition, the dynamic transition of the polarization spectrum from C6 to C3v line profile was observed in a cell culture in which norepinephrine induced an α- to β-myosin transition.

PMID: 31995740 [PubMed - as supplied by publisher]