Abstract
Background
This is a study about the skin aging exposome, focusing on the effect of cigarette smoke. Human living skin explants (HSE) were exposed to cigarette smoke (CS) of two cigarettes for 2 hours using a custom‐made exposure chamber, the Pollubox®. Effects on the surface physico‐chemistry and molecular properties of the skin were analyzed and reported for the first time.Basic Procedures
To this end, transcriptomic study followed by immunohistochemistry, malondialdehyde dosage (MDA) and surface physio‐chemistry data (surface free energy determination, TEWL, skin pH and FTIR spectroscopy of the explant) were collected from non‐treated and treated HSE.Main Findings
Results showed a decrease of the total surface free energy of the treated HSE. This decrease reflected higher interactions with polar compounds from the environment and consequently a decrease of the surface hydrophobicity. Additionally, an increase of TEWL and skin pH was observed after treatment.The transcriptomic analysis showed downregulation of mitochondrial genes (PON2‐NDUFA4L2‐ATP1A1‐ALDH2‐PRODH) combined with an increase of MDA in CS‐treated HSE.
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