MPs, Vol. 2, Pages 63: One-Step ARMS-PCR for the Detection of SNPs—Using the Example of the PADI4 Gene Methods and Protocols doi: 10.3390/mps2030063 Authors: Ehnert Linnemann Braun Botsch Leibiger Hemmann Nussler In eukaryotes, cellular functions are tightly controlled by diverse post-translational modifications (PTMs) of proteins. One such PTM affecting many proteins is the deimination of arginine to citrulline. This process, called citrullination is catalyzed...
Thu Jul 25, 2019 03:00
MPs, Vol. 2, Pages 62: Robust Normalization of Luciferase Reporter Data Methods and Protocols doi: 10.3390/mps2030062 Authors: Andrea Repele Manu Transient Luciferase reporter assays are widely used in the study of gene regulation and intracellular cell signaling. In order to control for sample-to-sample variation in luminescence arising from variability in transfection efficiency and other sources, an internal control reporter is co-transfected with the experimental reporter. The...
Thu Jul 25, 2019 03:00
MPs, Vol. 2, Pages 61: An Inexpensive Staining Alternative for Gelatin Zymography Gels Methods and Protocols doi: 10.3390/mps2030061 Authors: Christian Wechselberger Christian Doppler David Bernhard Zymography is a widely used electrophoretic method to determine proteolytic activities in samples from various sources. The method is based on copolymerizing a suitable protein substrate within a sodium dodecyl sulfate-polyacrylamide gel. Following electrophoretic separation of the...
Wed Jul 24, 2019 03:00
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