Contributors : Song-Tao Wu ; Yan-Fang YangSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensThe target proteins phosphoglycerate kinase 2 (PGK2), glycerol-3-phosphate dehydrogenase (GPD2)GPD2 and glucose-6-phosphate isomerase (GPI) were screened by combining transcriptome, proteomics and reverse dockingWe detected the binding constant of the active compound using microscale thermophoresis (MST). It was found that esculetin bound well with three potential target...
1h
Contributors : Amander Clark ; Wanlu Liu ; Na SunSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Homo sapiensIn vitro gametogenesis is the process of making germline cells from human pluripotent stem cells. The foundation of this model is the quality of the first progenitors called primordial germ cells (PGCs), which in vivo are specified during the peri-implantation window of human development. Here, we show using human embryo attachment culture that...
1h
Contributors : Yu Hayashi ; Shigeyoshi ItoharaSeries Type : Expression profiling by arrayOrganism : Mus musculusMicroarray identifies enriched expression of Nts in SubLDT, compared to dorsal and ventral area
1h
Contributors : Zhaoli Sun ; Jiang QianSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensAlcoholic hepatitis (AH) is frequently a severe clinical condition associated with high short-term mortality. This study was meant to serve as a resource for AH investigators in an effort to increase understanding of this disease and treatment research (NIH Project # 5R24AA025017).
1h
Contributors : Lioux Ghislaine ; Torres MiguelSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusThe mammalian heart contains multiple cell types that appear progressively during embryonic development. Advances in determining cardiac lineage diversification has often been limited by the unreliability of genetic tracers. Here we combine clonal analysis, genetic lineage tracing, tissue transplantation and mutant characterization to investigate the lineage relationships...
1h
Contributors : Lindsey T Brinton ; Rosa Lapalombella ; James S BlachlySeries Type : OtherOrganism : Homo sapiensCRISPR knockout screening on MOLM-13 cells with midostaurin or gilteritinib
1h
Contributor : Xiaodong MaSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusPurpose: we used next generation sequencing to analyze gene expression profiles of MEF treated with PBS (control), TNF-ɑ + Cycloheximide (TC) or TNF-ɑ + Cycloheximide+ z-VAD-fmk (TCZ). The goals of this study are to compare the different gene expression profiles between MEF treated with TC compared to PBS treatment and MEF treated with TCZ compared to PBS treatment.Methods: In MEF, the...
1h
Contributors : Hanan S Elsarraj ; Yan Hong ; Jeffrey Thompson ; Fariba BehbodSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Homo sapiensThere is limited research on which specific Wnt targets are regulated by B-Cell Lymphoma-9 (BCL9) and whether BCL9 regulates other signaling pathways to drive cancer malignancy. Here, using ChIP-Exo we have demonstrated that BCL9 in a protein complex with STAT3 drives DCIS invasive progression by regulation of enhancers...
1h
Contributors : Adriano Biasini ; Stefano de Pretis ; Jennifer Y Tan ; Baroj Abdulkarim ; Harry Wischnewski ; Rene Dreos ; Mattia Pelizzola ; Constance Ciaudo ; Ana C MarquesSeries Type : Non-coding RNA profiling by high throughput sequencing ; Expression profiling by high throughput sequencingOrganism : Mus musculusPosttranscriptional repression by microRNA (miRNA) occurs through transcript destabilization or translation inhibition. Whereas RNA degradation explains most miRNA-dependent repression,...
1h
Contributor : Sarah BoothSeries Type : Methylation profiling by genome tiling arrayOrganism : Homo sapiensDNA methylation data were assayed using the Illumina MethylationEPIC microarray platform in blood samples from 48 individuals taking part in a vitamin K supplementation trial.
1h
Contributors : Satoshi Kozawa ; Fumihiko Sagawa ; Satsuki Endo ; Glicia M Almeida ; Yuto Mitsuishi ; Thomas N SatoSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusApproximately 90% of pre-clinically validated drugs fail in clinical trials due to unanticipated clinical outcomes, costing over several hundred million US dollars per drug. Despite such critical importance, translating pre-clinical data to clinical outcomes remain a major challenge. Herein, we...
1h
Contributors : Sari Kassem ; Paolo Ferrari ; Amanda L Hughes ; Julien Soudet ; Oliver J Rando ; Michel StrubinSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Saccharomyces cerevisiaeTranscription in eukaryotes involves major chromatin changes including the replacement of old nucleosomal histones by new histones at the promoters of genes. The role of these histone exchange events in transcription remains unclear. In particular, it is unknown if histone...
1h
Contributors : Ju E Jeon ; Jung-Gun Kim ; Mary B Mudgett ; Elizabeth SattelySeries Type : Expression profiling by high throughput sequencingOrganism : Solanum lycopersicumIn response to biotic stress, plants produce suites of highly modified fatty acids that bear unusual chemical functionality. Despite their chemical complexity and proposed roles in pathogen defense, little is known about the biosynthesis of these decorated fatty acids in plants. Falcarindiol is a prototypical acetylenic lipid...
1h
Contributors : Xiumeng Hua ; Jiangping SongSeries Type : Expression profiling by high throughput sequencing ; Non-coding RNA profiling by high throughput sequencingOrganism : Homo sapiensBackground: Heart failure (HF) has been recognized as global pandemic with a high rate of associated hospitalization, morbidity and mortality. Although numerous advances have been made, its representative molecular signatures remain largely unknown, especially the role of genes in HF progression. The aim of the...
7h
Contributors : Christopher Martyniuk ; Ondrej AdamovskySeries Type : Expression profiling by arrayOrganism : Danio rerioPhthalate esters, such as DEHP (bis(2-ethylhexyl) phthalate, are a ubiquitous class of chemicals used as plasticizers in plastic products such as toys and hospital supplies. They are not covalently bound to the polymer matrix and can leach out of products. The goal of this study is to determine whether DEHP adversely affect the zebrafish (Danio rerio) microbiome-gut axis, specifically...
8h
Series Type : Expression profiling by high throughput sequencingOrganism : Mus musculusThis SuperSeries is composed of the SubSeries listed below.
9h
Contributors : Yi-Li Min ; Venkat S Malladi ; Miao Cui ; Zhaoning Wang ; Rhonda Bassel-Duby ; Eric N Olspm ; Ning LiuSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusTwist transcription factors function as ancestral regulators of mesodermal cell fates in organisms ranging from Drosophlia to mammals. Through lineage tracing of Twist2 (Tw2) expressing cells with tamoxifen-inducible Tw2-CreERT2 and tdTomato reporter mice, we discovered a unique cell population...
9h
Contributors : Yi-Li Min ; Venkat S Malladi ; Miao Cui ; Zhaoning Wang ; Ning Liu ; Rhonda Bassel-Duby ; Eric N Olson ; Ning LiuSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusTwist transcription factors function as ancestral regulators of mesodermal cell fates in organisms ranging from Drosophlia to mammals. Through lineage tracing of Twist2 (Tw2) expressing cells with tamoxifen-inducible Tw2-CreERT2 and tdTomato reporter mice, we discovered a unique...
9h
Series Type : Non-coding RNA profiling by high throughput sequencing ; Expression profiling by high throughput sequencingOrganism : Mus musculusThis SuperSeries is composed of the SubSeries listed below.
10h
Contributors : Carlos Perez-Cervantes ; Rangarajan D Nadadur ; Linsin A Smith ; Ivan P MoskowitzSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusWe performed mRNA transcriptional profiling of mouse retina in the wild-type and Nrl-null context to determine Nrl-dependent gene expressionWe sequenced cDNA libraries made from polyA+ selected RNA from retinas of litter-matched WT and Nrl-/- adult mice at postnatal day 21 (P21) (WT vs Nrl-/-, n=5 and n= 6 resp.)
10h
Contributors : Carlos Perez-Cervantes ; Linsin A Smith ; Rangarajan D Nadadur ; Ivan P MoskowitzSeries Type : Non-coding RNA profiling by high throughput sequencingOrganism : Mus musculusWe performed non-coding transcriptional profiling to identify Nrl-dependent ncRNAs by deep sequencing non-polyadenylated RNA from the same control and Nrl-/- retinal samples. non-coding RNA profiling is a robust method for the identification of cell-type specific functional enhancer elements in the mouse retina
10h
Contributors : Audrey Gasch ; James HoseSeries Type : Expression profiling by high throughput sequencingOrganism : Saccharomyces cerevisiaeAneuploidy, in which cells carry an abnormal chromosome count, is detrimental during development yet common in human cancers; why cells differ in tolerance remains unclear. We mapped the genetic basis of aneuploidy tolerance in wild Saccharomyces cerevisiae versus the sensitive lab strain to Ssd1, an RNA-binding protein involved in translation whose loss recapitulates...
10h
Contributors : Dmitry N Lyabin ; Ivan V Kulakovskiy ; Irina A EliseevaSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensWe present results of RNA-Seq, Ribo-Seq, and RIP-Seq (YB-1, YB-3) experiments performed in HEK293T cells, as well as in HEK293T cells with YB-1 knockout and overexpression. The data shows YB-1 function as a global translation inhibitor and YB-3 ability to substitute YB-1 in its function in YB-1 knockout mutant.
12h
Series Type : Expression profiling by high throughput sequencing ; Genome binding/occupancy profiling by high throughput sequencingOrganism : Saccharomyces cerevisiaeThis SuperSeries is composed of the SubSeries listed below.
13h
Series Type : Expression profiling by high throughput sequencing ; Genome binding/occupancy profiling by high throughput sequencingOrganism : Mus musculusThis SuperSeries is composed of the SubSeries listed below.
13h
Contributors : Wenlin Yuan ; Zora Modrusan ; Steffen DurinckSeries Type : Expression profiling by arrayOrganism : Mus musculuscompare expression of two groups of tumors
13h
Contributors : Francisco Nunez ; Rennolds S Ostrom ; Timothy B Johnstone ; Moom R RoosanSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensBudesonide alter cell function through gene expression changes that occur via activation of ubiquitously expressed intracellular glucocorticoid receptors. However, a number of recent studies suggest that budesonide also activate rapid alterations in various signaling processes that appear to be non-genomic in nature, and likely...
13h
Contributors : Matteo Barcella ; Daniele Braga ; Cristina BarlassinaSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensCirculatory shock affects approximately one-third of all patients in the Intensive Care Unit (ICU) and it is correlated with high mortality. Septic shock (SS) and cardiogenic shock (CS) are two types of circulatory shock, with a different etiology. We aimed to assess in whole blood of CS and SS patients the transcriptomic alterations occurring...
13h
Contributors : Wenlin Yuan ; Zora Modrusan ; Leonard D Goldstein ; Steffen DurinckSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusvalidate and study CRISPR/Cas9 knock down line by RNA-seq
13h
Contributors : Larissa Haertle ; Tobias Müller ; Roy Lardenoije ; Anna Maierhofer ; Marcus Dittrich ; Renzo J Riemens ; Samantha Stora ; Mathilde Roche ; Markus Leber ; Steffi Riedel-Heller ; Michael Wagner ; Martin Scherer ; Aimé Ravel ; Clotilde Mircher ; Cecile Cieuta-Walti ; Sophie Durand ; Daniel L van de Hove ; Per Hoffmann ; Alfredo Ramirez ; Thomas Haaf ; Nady El Hajj ; André MégarbanéSeries Type : Methylation profiling by arrayOrganism : Homo sapiensMethylomic profiling...
13h
Contributors : Ruby Y Huang ; Jieru Ye ; Tuan Z TanSeries Type : Expression profiling by arrayOrganism : Homo sapiensOvarian clear cell carcinoma (OCCC) is an epithelial ovarian cancer (EOC) histology having distinct pathology, biology, and molecular footprints. OCCC is chemo-resistant and has the worst stage-adjusted prognosis amongst EOC. Yet, treatment for OCCC patients is no different than other EOC. As OCCC incidence rate has significantly increase in recent decades, it is critical to find...
13h
Contributors : Anjana Yadav ; Ronald CollmanSeries Type : Expression profiling by arrayOrganism : Homo sapiensBackground. HIV-infected individuals on antiretroviral therapy (HIV/ART) experience higher levels of non-AIDS morbidity than uninfected people, which is believed to be driven by inflammation that persists despite viral suppression. Elevations in plasma cytokines, coagulation markers and both innate and adaptive immune cell activation during untreated infection are often incompletely...
13h
Series Type : Expression profiling by array ; Expression profiling by high throughput sequencingOrganism : Mus musculusThis SuperSeries is composed of the SubSeries listed below.
13h
Contributors : Wenlin Yuan ; Zora Modrusan ; Leonard D Goldstein ; Steffen DurinckSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusStudy gene expression in mouse mammary tumors single cell RNA-seq, study CRISPR/Cas9 knock down line by RNA-seq
13h
Contributors : Wei Wang ; Gang Ren ; Ni Hong ; Keji Zhao ; Wenfei JinSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusIn this study, we knockdown the expression of CTCF in EL4 cells by shRNA, followed by single cell RNA-seq on both wild type (WT) cells and CTCF-Knockdown (CTCF-KD) cells. Principal component analysis (PCA) of single cell RNA-seq data showed that WT cells and CTCF-KD cells essentially concentrated in two different clusters on the PCA projection,...
13h
Contributors : A Zumaquero ; S Kanematsu ; H Nakayashiki ; A Matas ; E Martínez-Ferri ; A Barceló ; F Pliego-Alfaro ; C López-Herrera ; F M Cazorla ; C PliegoSeries Type : Expression profiling by high throughput sequencingOrganism : Rosellinia necatrixWhite root rot disease caused by Rosellinia necatrix is one of the most important pathogens affecting avocado productivity in temperate, tropical and subtropical climates. Control of this disease is complex and nowadays, lies in the use of...
13h
Contributors : Xuan Bi ; YunFeng PanSeries Type : Expression profiling by array ; Non-coding RNA profiling by arrayOrganism : Homo sapiensRheumatoid arthritis (RA) is a common chronic inflammatory joint disease characterized by persistent synovial hyperplasia and progressive destruction of joint cartilage and bone.Fibroblast-like synoviocytes (FLSs), a prominent component of hyperplastic synovial pannus tissue, are the primary effector cells in RA synovial hyperplasia and invasion. However, the...
14h
Contributors : Jeremy Kiripolsky ; Rose-Anne Romano ; Eileen M Kasperek ; Guan Yu ; Jill M KramerSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusToll-like receptors (TLRs) are important mediators of chronic inflammation in numerous autoimmune diseases, although the role of these receptors in primary Sjögren’s syndrome (pSS) remains incompletely understood. Previous studies in our laboratory established Myd88 as a crucial mediator of disease, although the...
14h
Contributors : Bàrbara Reynés ; Evert M van Schothorst ; Jaap Keijer ; Paula Oliver ; Andreu PalouSeries Type : Expression profiling by arrayOrganism : Mustela putorius furoMale ferrets, aged 3 months, were divided into two group: one group remained at 22 degrees Celsius, while the other group was acclimatized to 4 degrees Celsius for one week. After sacrification, inguinal and periaortic white adipose tissues were dissected, and peripheral blood mononuclear cells (PBMC) were isolated. The three...
14h
Contributors : Elena Martynova ; Yilin Zhao ; Qing Xie ; Paul A Overbeek ; Deyou Zheng ; Ales CveklSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusGata3 is a DNA-binding transcription factor involved in cellular differentiation in a variety of tissues. To investigate the role of Gata3 in lens development we conducted lens-specific conditional loss-of-function model of Gata3 and confirmed abnormal regulation of cell cycle exit-coupled lens differentiation...
14h
Contributors : Ferrán Sánchez-Sanuy ; Cristina Peris-Peris ; Shiho Tomiyama ; Kazunori Okada ; Yue-Ie Hsing ; Blanca San Segundo ; Sonia CampoSeries Type : Expression profiling by high throughput sequencingOrganism : Oryza sativaMicroRNAs (miRNAs) are small non-coding RNAs that function as post-transcriptional regulators of gene expression in eukaryotes. In rice, miR7695 targets an alternatively spliced transcript of natural resistance-associated macrophage protein 6 (OsNramp6) encoding an...
14h
Contributors : Albert C Shaw ; Subhasis Mohanty ; Samit R Joshi ; Ikuyo Ueda ; Jean Wilson ; Juilee Thakar ; Hailong Meng ; Steven Kleinstein ; Sui TsangSeries Type : Expression profiling by arrayOrganism : Homo sapiensWe profiled gene expression from a stratified cohort of subjects to define influenza vaccine response in Young and Old.
15h
Contributors : Mercedes Schroeder ; Tokuji Tsuchiya ; Thomas EulgemSeries Type : Expression profiling by high throughput sequencingOrganism : Arabidopsis thalianaMacrophomina phaseolina (Mp) is a soil-borne pathogenic fungus known to infect more than 500 plants species including important crops. Here we report the use of a novel agar plate-based pathosystem using the model plant Arabidopsis thaliana (Arabidopsis) to study plant defense reponses to Mp, specifically a comparison between wild type...
15h
Contributors : Albert C Shaw ; Subhasis Mohanty ; Hailong Meng ; Steven Kleinstein ; Sui Tsang ; Stefan AveySeries Type : Expression profiling by arrayOrganism : Homo sapiensWe profiled gene expression from a stratified cohort of subjects to define influenza vaccine response in Young and Old
15h
Contributors : Albert C Shaw ; Subhasis Mohanty ; Samit R Joshi ; Ikuyo Ueda ; Jean Wilson ; Juilee Thakar ; Hailong Meng ; Steven Kleinstein ; Sui TsangSeries Type : Expression profiling by arrayOrganism : Homo sapiensWe profiled gene expression of B and T cells from a stratified cohort of subjects to define influenza vaccine response in Young and Old
15h
Contributors : Albert C Shaw ; Subhasis Mohanty ; Samit R Joshi ; Ikuyo Ueda ; Jean Wilson ; Juilee Thakar ; Hailong Meng ; Steven Kleinstein ; Sui TsangSeries Type : Expression profiling by arrayOrganism : Homo sapiensWe profiled gene expression from a stratified cohort of subjects to define influenza vaccine response in Young and Old.
15h
Series Type : Non-coding RNA profiling by high throughput sequencing ; Expression profiling by high throughput sequencingOrganism : Oryza sativa ; Oryza sativa Japonica GroupThis SuperSeries is composed of the SubSeries listed below.
15h
Contributor : Houqing ZengSeries Type : Expression profiling by high throughput sequencingOrganism : Oryza sativaUsing Illumina’s high-throughput small RNA sequencing and RNA sequencing, miRNAs and mRNAs differentially expressed under Zn deficiency and Zn resupply conditions were identified in shoots and roots of rice seedlings. A total of 68 miRNAs were identified to be differentially expressed under Zn deficiency and/or Zn resupply, and 38 of them were novel miRNAs. The Zn-responsive miRNAs were...
15h
Contributors : Robin Mesnage ; Christian VelotSeries Type : Expression profiling by arrayOrganism : Aspergillus nidulansIn order to evaluate the molecular effects of Roundup at a concentration at which no morphological effects were identified, RNA extracted from Roundup-treated A. nidulans cultures were subjected to a global gene expression profiling using a custom agilent microarray (8 × 15 K format), for which the reproducibility, specificity and sensitivity was described previously (Delomenie...
15h
Contributor : Houqing ZengSeries Type : Non-coding RNA profiling by high throughput sequencingOrganism : Oryza sativa Japonica GroupUsing Illumina’s high-throughput small RNA sequencing and RNA sequencing, miRNAs and mRNAs differentially expressed under Zn deficiency and Zn resupply conditions were identified in shoots and roots of rice seedlings. A total of 68 miRNAs were identified to be differentially expressed under Zn deficiency and/or Zn resupply, and 38 of them were novel miRNAs. The Zn-responsive...
15h
Contributors : Rebecca L Porter ; Neelima Magnus ; Vishal Thapar ; Robert Morris ; Annamaria Szabolcs ; Azfar Neyak ; Anupriya S Kulkarni ; Eric Tai ; Abhijit Chougule ; Allesandra Hillis ; Gabriel Golczer ; Hongshan Guo ; Teppei Yamada ; Tomohiro Kurokawa ; Chittampalli Yashaswini ; Matteo Ligorio ; Kevin D Vo ; Linda Nieman ; Andrew Liss ; Vikram Deshpande ; Michael S Lawrence ; Shyamala Maheswaran ; Carlos Fernandez-Del Castillo ; Theodore S Hong ; David P Ryan ; Peter J O'Dwyer...
15h
Contributors : D F Lawir ; K Sikora ; T BoehmSeries Type : Expression profiling by high throughput sequencingOrganism : Danio rerioIn mammals, T cell development in the thymus is critically dependent on the presence of the IL7 cytokine. Here, we describe the identification of the zebrafish ortholog of mammalian IL7, based on chromosomal localization, expression patterns, and deduced protein sequence. To examine the biological role of il7 in teleosts, we generated an il7 allele lacking most of its...
15h
Contributors : Zhulong Chan ; Wenli QuanSeries Type : Expression profiling by high throughput sequencingOrganism : Arabidopsis thalianaPurpose: We aimed to dissect function of Medicago truncutula homeodomain finger protein, MtPHD6 in ArabidopsisMethods: A total amount of 3 μg RNA was used for generation of sequencing libraries using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample....
15h
Contributors : Neil P Blackledge ; Nadezda A Fursova ; Angelika Feldmann ; Robert J KloseSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Mus musculusThe Polycomb repressive system is an essential chromatin-based regulator of gene expression. Despite being extensively studied, how it selects its target genes is poorly understood and whether its histone modifying activities are required for transcriptional repression remains controversial. Here, we directly...
16h
Series Type : Expression profiling by high throughput sequencing ; Genome binding/occupancy profiling by high throughput sequencing ; OtherOrganism : Mus musculusThis SuperSeries is composed of the SubSeries listed below.
16h
Contributors : Neil P Blackledge ; Nadezda A Fursova ; Angelika Feldmann ; Robert J KloseSeries Type : Expression profiling by high throughput sequencing ; OtherOrganism : Mus musculusThe Polycomb repressive system is an essential chromatin-based regulator of gene expression. Despite being extensively studied, how it selects its target genes is poorly understood and whether its histone modifying activities are required for transcriptional repression remains controversial. Here, we directly test...
16h
Contributors : Neil P Blackledge ; Nadezda A Fursova ; Angelika Feldmann ; Robert J KloseSeries Type : OtherOrganism : Mus musculusThe Polycomb repressive system is an essential chromatin-based regulator of gene expression. Despite being extensively studied, how it selects its target genes is poorly understood and whether its histone modifying activities are required for transcriptional repression remains controversial. Here, we directly test the requirement for PRC1 catalytic activity in Polycomb...
16h
Contributors : Alison P Casserly ; Junko Tsuji ; Paul D GardnerSeries Type : Expression profiling by high throughput sequencing ; Non-coding RNA profiling by high throughput sequencingOrganism : Mus musculusNicotine dependence is responsible for perpetuating the adverse health effects due to tobacco use, the leading cause of preventable death worldwide. Nicotine is an agonist for nicotinic acetylcholine receptors, which are enriched in the habenulo-interpeduncular withdrawal circuit. Drugs of abuse,...
17h
Contributors : Oliver H Bell ; David A Copland ; Amy Ward ; Lindsay B Nicholson ; Clemens A Lange ; Colin J Chu ; Andrew D DickSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusBackground: Whether retinal microglia can maintain or restore immune homeostasis during and after inflammation is unclear. We performed single-eye mRNA-sequencing on microglia at different timepoints following a single inflammatory stimulus to characterise their transcriptome during and...
17h
Contributors : Máté Pálfy ; Gunnar Schulze ; Eivind Valen ; Nadine L VastenhouwSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Danio rerioIn many organisms, early embryonic development is driven by maternally provided transcription factors until the controlled onset of transcription during zygotic genome activation (ZGA). The regulation of chromatin accessibility and its relationship to gene activity during this transition remains poorly understood. Here,...
18h
Contributors : Yicheng Wang ; Taroh KinoshitaSeries Type : Expression profiling by arrayOrganism : Homo sapiensTranscriptome analysis of total RNA samples from HEK293-PIGS-KO and HEK293-PIGS-UBE2J1-DKO cells. To check whether KO of UBE2J1 upregulates genes of GPI biosthesis pathway, we used microarrays to analyze gene expression change by KO of UBE2J1 and comfirmed that known GPI pathway genes are not changed by ERAD-deficiency.
20h
Contributors : Johnny Bonnardel ; Clément Da Silva ; Hugues LelouardSeries Type : Expression profiling by arrayOrganism : Mus musculusThe specialized monocyte-derived phagocytes termed LysoDC are hallmarks of Peyer’s patches where their main function is to sample pathogens. However, their differentiation pathway and migratory properties remain uncharacterized. From single-cell RNA sequencing, we built LysoDC differentiation trajectories and fate diversity in correlation with their location and...
23h
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