In Vitro Stimulation and Visualization of Extracellular Trap Release in Differentiated Human Monocyte-derived Macrophages

Presented here is a protocol to detect macrophage extracellular trap (MET) production in live cell culture using microscopy and fluorescence staining. This protocol can be further extended to examine specific MET protein markers by immunofluorescence staining.

JoVE: Journal of Visualized Experiments

Fri Nov 01, 2019 21:24

Labeling and Imaging of Amyloid Plaques in Brain Tissue Using the Natural Polyphenol Curcumin

Curcumin is an ideal fluorophore for labeling and imaging of amyloid beta protein plaques in brain tissue due to its preferential binding to amyloid beta protein as well as its structural similarities with other traditional amyloid binding dyes. It can be used to label and image amyloid beta protein plaques more efficiently and inexpensively than traditional methods.

JoVE: Journal of Visualized Experiments

Fri Nov 01, 2019 21:00

Palatable Western-style Cafeteria Diet as a Reliable Method for Modeling Diet-induced Obesity in Rodents

This protocol describes the use of a highly palatable, western-style cafeteria diet to model overeating and obesity in rodents. Here, we provide a detailed outline of food selection, preparation and measurement, and explain methodological factors that assist in generating a robust and reproducible phenotype.

JoVE: Journal of Visualized Experiments

Fri Nov 01, 2019 20:23

Statistical Modelling of Cortical Connectivity Using Non-invasive Electroencephalograms

Standard EEG analysis techniques offer limited insight into nervous system function. Deriving statistical models of cortical connectivity offers far greater ability to investigate underlying network dynamics. Improved functional assessment opens new possibilities for diagnosis, prognostication, and outcome prediction in nervous system diseases.

JoVE: Journal of Visualized Experiments

Fri Nov 01, 2019 20:00

SUMO-Binding Entities (SUBEs) as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer

Here, we present a protocol to enrich, isolate, identify, and characterize proteins modified by SUMO in vivo both from human hepatoma cells and liver tumors obtained from mouse models of hepatocellular carcinoma by using SUMO-binding entities (SUBEs).

JoVE: Journal of Visualized Experiments

Fri Nov 01, 2019 19:22

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Altered nuclease activity has been associated with different human conditions, underlying its potential as a biomarker. The modular and easy to implement screening methodology presented in this paper allows the selection of specific nucleic acid probes for harnessing nuclease activity as a biomarker of disease.

JoVE: Journal of Visualized Experiments

Fri Nov 01, 2019 19:00

Are sweetened drinks a gateway to alcohol, opiate and stimulant addiction? Summary of evidence and therapeutic strategies

Publication date: Available online 31 October 2019Source: Medical HypothesesAuthor(s): Prasanna N. de SilvaAbstract1. Drinks sweetened with both sugar and artificial additives lead to dopamine release at the nucleus accumbens (NAc) in rat models; the basis of experiences of pleasure in humans, resulting in impulsive binging behaviour at times.2. Evidence from rat models show cross sensitisation between sweetened drinks, alcohol, opiates and stimulants. Therefore, it could be hypothesised that sweetened...

ScienceDirect Publication: Medical Hypotheses

Fri Nov 01, 2019 14:45

Personality Changes Following Heart Transplantation: The Role of Cellular Memory

Publication date: Available online 31 October 2019Source: Medical HypothesesAuthor(s): Mitchell B. LiesterAbstractPersonality changes following heart transplantation have been reported for decades and include accounts of recipients acquiring the personality characteristics of their donor. Four categories of personality changes are discussed: (1) changes in preferences, (2) alterations in emotions/temperament, (3) modifications of identity, and (4) memories from the donor’s life. The acquisition of...

ScienceDirect Publication: Medical Hypotheses

Fri Nov 01, 2019 14:45