Neoantigen-activated haploidentical T cell therapy for angioimmunoblastic T-cell lymphoma Publication date: January 2020 Source: Clinical Immunology, Volume 210 Author(s): Yuan Chen, Haimei Chen, Weiyue Gu, Guoyu Hu |
Screening of tumor-associated antigens based on Oncomine database and evaluation of diagnostic value of autoantibodies in lung cancer Publication date: January 2020 Source: Clinical Immunology, Volume 210 Author(s): Tingting Wang, Hongchun Liu, Lu Pei, Kaijuan Wang, Chunhua Song, Peng Wang, Hua Ye, Jianying Zhang, Zhenyu Ji, Songyun Ouyang, Liping Dai AbstractObjectivesThe purpose of this study is to discover novel tumor-associated antigens (TAAs) to improve the diagnosis of lung cancer (LC).Materials and methodsOncomine database was used to discover potential TAAs from LC tissues, enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of autoantibodies against TAAs in two independent sets (identification set, n = 368; validation set, n = 1011).ResultsAnalyses of sera from identification set showed that the sensitivity of autoantibodies against five TAAs (HMGB3, ZWINT, GREM1, NUSAP1 and MMP12) reached 57.1%, 42.4%, 38.0%, 36.4% and 20.7%, with area under ROC curve (AUC) of 0.85, 0.75, 0.71, 0.73 and 0.70, respectively. It also validated the diagnostic performances of these autoantibodies with AUC of 0.72, 0.65, 0.61, 0.64 and 0.64, respectively. Autoantibody against HMGB3 exhibited significantly increased frequency in early LC (53.3%) compared to advanced LC (29.3%) (P < .05). The positive rates of autoantibody against HMGB3 and NUSAP1 in serum of LC patients without distant metastasis were significantly higher than that of distant metastatic LC (P < .05). When each of the three protein biomarkers (CEA, CA125 and CYFRA21-1) was combined with anti-HMGB3 autoantibody, the sensitivity of early LC increased to 72.7%, 63.3% and 75.9% from 36.4%, 13.3% and 27.6%, respectively.ConclusionAutoantibodies against 5 TAAs (HMGB3, ZWINT, GREM1, NUSAP1 and MMP12) might have favorable diagnostic values in LC detection, and autoantibody against HMGB3 has the potential to serve as a serological biomarker in early-stage LC. The combination of protein biomarkers and anti-HMGB3 might contribute to detection of early-stage LC. |
A novel NFKBIA variant substituting serine 36 of IκBα causes immunodeficiency with warts, bronchiectasis and juvenile rheumatoid arthritis in the absence of ectodermal dysplasia Publication date: January 2020 Source: Clinical Immunology, Volume 210 Author(s): Georgios Sogkas, Ignatius R. Adriawan, Felix C. Ringshausen, Ulrich Baumann, Claudia Schröder, Christian Klemann, Sandra von Hardenberg, Gunnar Schmidt, Auber Bernd, Alexandra Jablonka, Diana Ernst, Reinhold E. Schmidt, Faranaz Atschekzei Abstract
Genetic studies have led to identification of an increasing number of monogenic primary immunodeficiency disorders. Monoallelic pathogenic gain-of-function (GOF) variants in NFKBIA, the gene encoding IκBα, result in an immunodeficiency disorder, typically accompanied by anhidrotic ectodermal dysplasia (EDA). So far, 14 patients with immunodeficiency due to NFKBIA GOF mutations have been reported. In this study we report three patients from the same family with immunodeficiency, presenting with recurrent respiratory tract infections, bronchiectasis and viral skin conditions due to a novel pathogenic NFKBIA variant (c.106 T > G, p.Ser36Ala), which results in reduced IκBα degradation. Immunological investigations revealed inadequate antibody responses against vaccine antigens, despite hypergammaglobulinemia. Interestingly, none of the studied patients displayed features of EDA. Therefore, missense NFKBIA variants substituting serine 36 of IκBα, differ from the rest of pathogenic GOF NFKBIA variants in that they cause combined immunodeficiency, even in the absence of EDA.
|
An update on genetic susceptibility in lupus nephritis Publication date: January 2020 Source: Clinical Immunology, Volume 210 Author(s): Kangkang Song, Lu Liu, Xuejun Zhang, Xiangmei Chen Abstract
Systemic lupus erythematosus (SLE) is a chronic autoimmune disease characterized by multiple system involvement and positive serum autoantibodies. Lupus nephritis (LN) is the most common and serious complication of SLE, and it is the main cause of death in patients with SLE. Abnormalities in the immune system lead to LN and involve a variety of cells (T cells, B cells, macrophages, NK cells, etc.), cytokines (interleukin, tumor necrosis factor α, etc.) and their related pathways. Previous studies have shown that the interactions of genetic, epigenetic and environmental factors contribute to the pathogenesis and development of LN. In recent years, one genome-wide association study (GWAS) and a number of gene association studies have explored the susceptibility genes of LN, including immunization-, inflammation-, adhesion- and other pathway-related genes. These genes participate in or suggest the pathogenesis and progression of LN. In this review, we summarize the genetic susceptibility of LN and discuss the possible mechanism underlying the susceptibility genes of LN.
|
Sepsis roadmap: What we know, what we learned, and where we are going Publication date: January 2020 Source: Clinical Immunology, Volume 210 Author(s): Vijay Kumar Abstract
Sepsis is a life-threatening condition originating as a result of systemic blood infection causing, one or more organ damage due to the dysregulation of the immune response. In 2017, the world health organization (WHO) declared sepsis as a disease of global health priority, needing special attention due to its high prevalence and mortality around the world. Most of the therapeutics targeting sepsis have failed in the clinics. The present review highlights the history of the sepsis, its immunopathogenesis, and lessons learned after the failure of previously used immune-based therapies. The subsequent section, where to go describes in details the importance of the complement system (CS), autophagy, inflammasomes, and microbiota along with their targeting to manage sepsis. These systems are interconnected to each other, thus targeting one may affect the other. We are in an urgent need for a multi-targeting therapeutic approach for sepsis.
Graphical abstract |
Prognostic role of circulating neutrophil extracellular traps levels for long-term mortality in new end-stage renal disease patients Publication date: January 2020 Source: Clinical Immunology, Volume 210 Author(s): Jwa-Kyung Kim, Hoi Woul Lee, Narae Joo, Hyung Seok Lee, Young Rim Song, Hyung Jik Kim, Sung Gyun Kim Abstract
Dysregulation of innate immunity has been proposed as an important contributing factor for advanced atherosclerosis and resultant high mortality in hemodialysis (HD) patients. To evaluate the long-term prognostic role of in vivo neutrophil extracellular traps (NETs), we measured circulating serum nucleosome, myeloperoxidase (MPO), and DNase I levels in 281 incident HD patients. Circulating nucleosome level was significantly higher in HD patients compared to controls, and it was closely associated with MPO levels, suggesting increased in vivo NETs in uremia. Patients in the nucleosome Q4 group had significantly increased all-cause and adverse CV mortality compared to those in the Q1–3 group even after adjusting traditional risk factors Also, serum DNase I level was significantly higher in HD patients than controls (2.76 ± 1.02 ng/ml and 1.93 ± 0.85 ng/ml), but it had no correlation with NETs. Interestingly, it serves an additive biomarker for predicting poor CV outcomes. The two novel biomarkers might provide an importance independent prognostic significance in incident HD patients.
|
Antagonistic role of IL-1ß and NLRP3/IL-18 genetics in chronic HIV-1 infection Publication date: December 2019 Source: Clinical Immunology, Volume 209 Author(s): Edione C. Reis, Vinicius N.C. Leal, Lais T. da Silva, Marilia M.L. dos Reis, Enrique R. Argañaraz, Telma M. Oshiro, Alessandra Pontillo Abstract
Host genetics affects both susceptibility and progression of HIV-1 infection. NLRP3 inflammasome provides a first-line defense in viral infections, and, accordingly, gain-of-function variants in NLRP3 have been associated with protection against HIV-1.
Despite antiretroviral treatment (ART), HIV-infected patients continue to present systemic inflammation with a heterogeneous prognosis. As NLRP3 inflammasome is involved in several chronic diseases by amplifying “sterile” inflammation, its role in chronic phase of HIV infection has been postulated. Little is known about inflammasome genetics in HIV-infected patients and whether it may play a role in the different clinical outcomes. Therefore, we questioned whether NLRP3 inflammasome genetics could affect the clinical course of HIV-1 infection as it does in host/virus interaction. For this purpose, we analyzed selected single nucleotide polymorphisms (SNPs) in ART-treated HIV-infected patients (n = 300), in Long Term Non-Progressors/Elite Controllers and progressors (n = 133), and in HIV-infected individuals submitted to dendritic cell (DC)-based immunotherapy (n = 19). SNPs leading to increased activation of NLRP3 inflammasome are beneficial for patients, while SNPs that negatively affect NLRP3 activation or IL-18 production, detrimental. In contrast, gain-of-function variant in IL1B is also detrimental for patients, suggesting that while IL-1ß possible contributes to immune exhaustion, the axis NLRP3-inflammasome/IL-18 could act positively in chronic infection. Functional assays supported genetic results: NLRP3 variants associated with good quality HIV+ DC, and IL1B -511C > T with a poor one. Loss-of-function SNPs affect HIV+ T cells proliferation. These findings proposed for the first time that NLRP3 inflammasome, mainly through IL-18, play a protective role in chronic HIV infection. |
Independent association of low IFNλ1 gene expression and type I IFN score/IFNλ1 ratio with obstetric manifestations and triple antiphospholipid antibody positivity in primary antiphospholipid syndrome Publication date: December 2019 Source: Clinical Immunology, Volume 209 Author(s): Christina-Maria Flessa, Stelios Vlachiotis, Adrianos Nezos, Evangelos Andreakos, Clio P. Mavragani, Maria G. Tektonidou Abstract
Recent data suggest an important role of type I interferons (IFN) in antiphospholipid syndrome (APS). Here we aimed to evaluate the interplay of type I and type III (or IFNλs) IFNs in APS and potential clinical and serological associations. Our findings suggest that patients with primary APS (PAPS) and systemic lupus erythematosus (SLE)/APS displayed increased type I IFN scores but decreased IFNλ1 gene expression levels compared to healthy individuals, as assessed with real-time qPCR analysis in isolated peripheral blood mononuclear cells (PBMCs). Type I IFN score/IFNλ1 ratio was remarkably higher in patients with PAPS and SLE/APS as well as in SLE patients with or without antiphospholipid antibodies (aPL) vs controls. In conclusion, our results reveal an association between low IFNλ1 expression and obstetric APS. Moreover, the type I IFN score/IFNλ1 ratio seems to be a potential marker of high risk APS given its associations with triple aPL positivity.
|
A novel tool for clinical diagnosis of allergy operating a microfluidic immunoaffinity basophil activation test technique Publication date: December 2019 Source: Clinical Immunology, Volume 209 Author(s): Zenib Aljadi, Frida Kalm, Caroline Nilsson, Ola Winqvist, Aman Russom, Joachim Lundahl, Anna Nopp Abstract
The Basophil Activation Test (BAT) is a valuable allergy diagnostic tool but is time-consuming and requires skilled personnel and cumbersome processing, which has limited its clinical use. We therefore investigated if a microfluidic immunoaffinity BAT (miBAT) technique can be a reliable diagnostic method. Blood was collected from allergic patients and healthy controls. Basophils were challenged with negative control, positive control (anti-FcεRI), and two concentrations of a relevant and non-relevant allergen. CD203c and CD63 expression was detected by fluorescent microscopy and flow cytometry. In basophils from allergic patients the CD63% was significantly higher after allergen activation as compared to the negative control (p<.0001–p=.0004). Activation with non-relevant allergen showed equivalent CD63% expression as the negative control. Further, the miBAT data were comparable to flow cytometry. Our results demonstrate the capacity of the miBAT technology to measure different degrees of basophil allergen activation by quantifying the CD63% expression on captured basophils.
|
Adjuvant effect of TLR7 agonist adsorbed on aluminum hydroxide (AS37): A phase I randomized, dose escalation study of an AS37-adjuvanted meningococcal C conjugated vaccine Publication date: December 2019 Source: Clinical Immunology, Volume 209 Author(s): Antonio Gonzalez-Lopez, Jaap Oostendorp, Thomas Koernicke, Tommaso Fadini, Ugo D'Oro, Sherryl Baker, Derek T. O’Hagan, Giuseppe Del Giudice, Emilio Siena, Oretta Finco, Duccio Medini Abstract
An adjuvant system (AS37) has been developed containing a synthetic toll-like receptor agonist (TLR7a). We conducted a phase I randomized, observer-blind, dose-escalation study to assess the safety and immunogenicity of an investigational AS37-adjuvanted meningococcus C (MenC) conjugate vaccine in healthy adults (NCT02639351). A control group received a licensed MenC conjugate alum-adjuvanted vaccine. Eighty participants were randomized to receive one dose of control or investigational vaccine containing AS37 (TLR7a dose 12.5, 25, 50, 100 μg). All vaccines were well tolerated, apart from in the TLR7a 100 μg dose group, which had three reports (18.8%) of severe systemic adverse events. Four weeks after vaccination, human complement serum bactericidal assay seroresponse rates against MenC were 56–81% in all groups, and ELISA seroresponses were ≥81% for all AS37-adjuvanted vaccine groups (100% in 50 and 100 μg dose groups) and 88% in the control group. Antibody responses were maintained at six months after vaccination.
|
Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,00306932607174,alsfakia@gmail.com,
Πληροφορίες
Ετικέτες
Τρίτη 12 Νοεμβρίου 2019
Αναρτήθηκε από
Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,00306932607174,alsfakia@gmail.com,
στις
11:05 μ.μ.
Ετικέτες
00302841026182,
00306932607174,
alsfakia@gmail.com,
Anapafseos 5 Agios Nikolaos 72100 Crete Greece,
Medicine by Alexandros G. Sfakianakis,
Telephone consultation 11855 int 1193
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
Αρχειοθήκη ιστολογίου
-
►
2023
(276)
- ► Φεβρουαρίου (133)
- ► Ιανουαρίου (143)
-
►
2022
(1976)
- ► Δεκεμβρίου (116)
- ► Σεπτεμβρίου (158)
- ► Φεβρουαρίου (165)
- ► Ιανουαρίου (161)
-
►
2021
(3661)
- ► Δεκεμβρίου (161)
- ► Σεπτεμβρίου (274)
- ► Φεβρουαρίου (64)
- ► Ιανουαρίου (368)
-
►
2020
(4554)
- ► Δεκεμβρίου (400)
- ► Σεπτεμβρίου (381)
- ► Φεβρουαρίου (638)
- ► Ιανουαρίου (691)
-
▼
2019
(4999)
- ► Δεκεμβρίου (924)
-
▼
Νοεμβρίου
(806)
- Ξηρή Μύτη
- Medicine by Alexandros G. Sfakianakis,Anapafseos 5...
- Medicine by Alexandros G. Sfakianakis,Anapafseos 5...
- We thank You, God, for the food we eat; We thank y...
- Medicine by Alexandros G. Sfakianakis,Anapafseos 5...
- International Journal of Molecular Sciences,
- International Journal of Environmental Resear...
- Antioxidants, Vol. 8, Pages 589: Synthesis...
- Melanocytic aggregates with unique morphology as...
- Fatal obstructive asphyxia: Trans-pulmonary dens...
- Geochronology of the southern Baltic Sea sedimen...
- Intraoperative Multipoint Acupuncture for Reduci...
- Enhanced expression of NLRP3 inflammasome compon...
- Mechanical and geometric properties of thermof...
- The Physician's Guide to Platelet-Rich Plasma in...
- Direct-acting Antivirals for the Treatment of Ki...
- Thyroid functions and levels of some trace eleme...
- In vivo and in vitro diagnosis of cracked teeth:...
- Association of colorectal surgeons of India: Pas...
- Fatal cases of animal-mediated human rabies: Loo...
- Strategy for Securing Key Patents in the Field o...
- Identification of passion fruit ( Passiflora edu...
- Journal of Real-Time Image Processing: sixth iss...
- The transient expression of CHIKV VLP in large s...
- Self-Compassion and Suicide Risk in Veterans: Whe...
- Nanoglomus plukenetiae , a new fungus from Peru,...
- Thinking (about) groups: a special issue of Synt...
- Athamanika or Tzoumerka is a mountain range in nor...
- American Journal of Roentgenology, Women's Imag...
- R-dihydroetorphine Analgesia and Respiratory Depre...
- Obstructive sleep apnea and cardiovascular dis...
- Ionic Liquid Forms of Carvedilol: Preparation, C...
- Mitochondrial tRNA Ser(UCN) 7471delC may be a no...
- Associations of Intraoperative Radial Arterial S...
- Cannabis Use Disorder and Perioperative Outcomes...
- Computed tomography diagnosis of transomental h...
- Spheno-orbital meningiomas, Letter of response t...
- Arteriovenous malformation associated with a HRA...
- Diversity of igneous rocks from the Isachsen Dom...
- Racial/ethnic and socioeconomic survival dispari...
- Harlekinsyndrom nach Skoliosechirurgie Zusammenf...
- Dental characteristics and according treatments...
- Editorial water history issue 3/4 2019 Technical...
- Experimentally Derived Sedimentary, Molecular, a...
- Comparison of Voice Quality of Life in Early Sta...
- Fibrinogen Concentrate vs Cryoprecipitate For...
- Poly(ADP‐ribose) polymerase‐1 depletion enhances...
- Chronic venous insufficiency: A review for nurs...
- Selective Dorsal Rhizotomy for the Treatment of ...
- Analysis of the Visual Perception of Female Brea...
- Improving Provider Compliance With Adult Inflamm...
- A Systematic Review of the Treatment of Superfic...
- Cannabinoids and inflammation: implications for ...
- Mass Casualties and Case ManagementThe mass casu...
- Relationships between organizational and individu...
- Enhanced Recovery After Surgery: Concepts and Ap...
- Neuraxial Block for Delivery Among Women With Lo...
- Ensuring Quality: Are We Doing Enough to Protect...
- Physical Activity and Pain in Youth With Sickle ...
- Patient Safety Culture, Health Information Techn...
- Taming Tinnitus: Addressing Mental Distress a Ke...
- Healthcare’s Present and Future: Consumer Center...
- Management of choledocholithiasis in a 5-month-o...
- Grey areas and evidence gaps in the management o...
- Medicine by Alexandros G. Sfakianakis,Anapafseos 5...
- Medicine by Alexandros G. Sfakianakis,Anapafseos 5...
- Living Arrangement and Economic Dependency among...
- Premalignant male genital dermatosesRochit Rajes...
- Effectiveness of a Pulmonary Rehabilitation ...
- Alcohol-mediated behaviours and the gut-brai...
- Comparison of the structural dynamic and mit...
- ScienceDirect Publication: International Immu...
- The Effects of Different Exercise Trainings ...
- Study tracks genomic changes that reinforce ...
- Laryngo-Rhino-Otologie,
- Update on Facial Skin Rejuvenation Technology
- Latest Results for Psychopharmacology
- International Journal of Environmental Resear...
- Psychological impact of working in paediatric i...
- Closure of the retroperitoneal space in laparosc...
- Nonpowder Firearm Injuries to Children Treate...
- Association between Smoking and 30-Day Outcomes...
- Implementation Strategies for Cardiovascular ...
- Otoscopic Clinic, Cavernous Hemangioma of the E...
- Ten Commandments of Safe and Optimum Neck Dissec...
- Emerging antimicrobial resistance and newer tool...
- Facial Skin Rejuvenation Technology,FACIAL PLAST...
- Conventional X-ray as an additional tool in the ...
- High heavy metal load does not inhibit nitrogen ...
- detection of Cryptosporidium and Giardia in the ...
- ► Σεπτεμβρίου (845)
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου