Related ArticlesThe endoscopic anatomy of the cochlear hook region and fustis: surgical implications. Acta Otorhinolaryngol Ital. 2019 Oct;39(5):353-357 Authors: Anschuetz L, Alicandri-Ciufelli M, Wimmer W, Bonali M, Caversaccio M, Presutti L PMID: 31708582 [PubMed - in process]
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Related ArticlesObservational study on risk factors determining residual dizziness after successful benign paroxysmal positional vertigo treatment: the role of subclinical BPPV. Acta Otorhinolaryngol Ital. 2019 Oct;39(5):347-352 Authors: Dispenza F, Mazzucco W, Mazzola S, Martines F PMID: 31708581 [PubMed - in process]
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Related ArticlesChronic rhinosinusitis with polyposis and serum vitamin D levels. Acta Otorhinolaryngol Ital. 2019 Oct;39(5):336-340 Authors: Bavi F, Movahed R, Salehi M, Hossaini S, Bakhshaee M PMID: 31708580 [PubMed - in process]
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Related ArticlesOlfactory dysfunction in patients with chronic rhinosinusitis with nasal polyps is associated with clinical-cytological grading severity. Acta Otorhinolaryngol Ital. 2019 Oct;39(5):329-335 Authors: Gelardi M, Piccininni K, Quaranta N, Quaranta V, Silvestri M, Ciprandi G PMID: 31708579 [PubMed - in process]
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Related ArticlesRecurrent Bell's palsy: outcomes and correlation with clinical comorbidities. Acta Otorhinolaryngol Ital. 2019 Oct;39(5):316-321 Authors: Mancini P, Bottaro V, Capitani F, De Soccio G, Prosperini L, Restaino P, De Vincentiis M, Greco A, Bertoli GA, De Seta D PMID: 31708578 [PubMed - in process]
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Related ArticlesClinical application of cVEMPs and oVEMPs in patients affected by Ménière's disease, vestibular neuritis and benign paroxysmal positional vertigo: a systematic review. Acta Otorhinolaryngol Ital. 2019 Oct;39(5):298-307 Authors: Scarpa A, Gioacchini FM, Cassandro E, Tulli M, Ralli M, Re M, Cassandro C PMID: 31708577 [PubMed - in process]
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Related ArticlesAssessment of obstructive sleep apnoea (OSA) in children: an update. Acta Otorhinolaryngol Ital. 2019 Oct;39(5):289-297 Authors: Savini S, Ciorba A, Bianchini C, Stomeo F, Corazzi V, Vicini C, Pelucchi S PMID: 31708576 [PubMed - in process]
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Related ArticlesKinetics and interrelations of the renin aldosterone response to acute psychosocial stress: a neglected stress system. J Clin Endocrinol Metab. 2019 Nov 10;: Authors: Gideon A, Sauter C, Fieres J, Berger T, Renner B, Wirtz PH Abstract CONTEXT: The Renin-Angiotensin-Aldosterone-System (RAAS) plays an important role in cardiovascular homeostasis and its dysfunction relates to negative health consequences. Acute psychosocial stress seems...
Tue Nov 12, 2019 17:20
Related ArticlesUnderstanding Thyroid Cell Stress. J Clin Endocrinol Metab. 2019 Nov 11;: Authors: Morshed SA, Davies TF Abstract Understanding the regulatory mechanisms that control intracellular stress has fundamental importance since its failure results in cell death. Evidence has emerged indicating that the intracellular signals that are induced in response to diverse stresses include the DNA damage response, the unfolded protein response, (UPR),...
Tue Nov 12, 2019 17:20
Related ArticlesCharacteristics of upright versus supine reflux pattern in patients with laryngopharyngeal reflux. Braz J Otorhinolaryngol. 2019 Sep 18;: Authors: Mesallam TA, Baqays AA Abstract INTRODUCTION: Many laryngeal-related problems have been attributed to laryngopharyngeal reflux including dysphonia, frequent throat clearing, chronic cough, and globus sensation. However, there is still controversy regarding diagnosis and clinical presentation...
Tue Nov 12, 2019 15:14
Related ArticlesResponse to Preserving the Military Medicine Workforce: One Team, One Fight. Mil Med. 2019 Nov 11;: Authors: Wiesen CAR, Marcum CRE, Soltis CMA, Peterson CKA PMID: 31711191 [PubMed - as supplied by publisher]
Tue Nov 12, 2019 17:04
Related ArticlesEfficacy of a Temporary Hemostatic Device in a Swine Model of Closed, Lethal Liver Injury. Mil Med. 2019 Nov 11;: Authors: Qin H, Yang L, Liu D, Chen S, Lyu M, Bao Q, Lai X, Liu H, Chen Q, Zong Z Abstract INTRODUCTION: Solid abdominal organ hemorrhage remains one of the leading causes of death both on the battlefield of modern warfare and in the civilian setting. A novel, temporary hemostatic device combining CELOX and direct intra-abdominal...
Tue Nov 12, 2019 17:04
Related ArticlesDurable response to palbociclib and letrozole in ovarian cancer with CDKN2A loss. Cancer Biol Ther. 2019 Nov 10;:1-6 Authors: Frisone D, Charrier M, Clement S, Christinat Y, Thouvenin L, Homicsko K, Michielin O, Bodmer A, Chappuis PO, McKee TA, Tsantoulis P Abstract Alterations of the Retinoblastoma (Rb) pathway are frequent in ovarian cancer, typically resulting from CDKN2A down-regulation, CCNE1 amplification, CCND1/2 amplification,...
Tue Nov 12, 2019 17:14
Related ArticlesBotryllamide G is an ABCG2 inhibitor that improves lapatinib delivery in mouse brain. Cancer Biol Ther. 2019 Nov 10;:1-8 Authors: Strope JD, Peer CJ, Sissung TM, Hall OM, Huang PA, Harris EM, Gustafson KR, Henrich CJ, Sigano DM, Pauly GT, Schneider JP, Bates SE, Figg WD Abstract Introduction: Transporters comprising the blood-brain barrier complicate delivery of many therapeutics to the central nervous system. The present study ascertained...
Tue Nov 12, 2019 17:14
Related ArticlesOverexpression of the Long Non-Coding RNA NeSt and FOXCUT in Early Stages of Prostate Cancer Samples as Promising Biomarkers. Clin Lab. 2019 Nov 01;65(11): Authors: Rostami S, Nabi M, Naghavi P, Mohammadzadeh N, Khalili A Abstract BACKGROUND: Prostate cancer is one of the most common cancers in males worldwide. Recently, it is well characterized that long non-coding RNAs (lncRNA) play critical roles in the initiation, development, and...
Tue Nov 12, 2019 17:19
Related ArticlesBetter Resolution of Gel Electrophoresis than that of Capillary Electrophoresis: About a Case Report. Clin Lab. 2019 Nov 01;65(11): Authors: Biaz A, Raiss C, El-Amin G, Uwingabiye J, El-Machtani-Idrissi S, Dami A, Ouzzif Z, Bouhsain S PMID: 31710450 [PubMed - in process]
Tue Nov 12, 2019 17:19
Related ArticlesIncreasing DNA Isolation Performance from Blood Cells Using a Modified Commercial Low-Cost Reagent. Clin Lab. 2019 Nov 01;65(11): Authors: Kraemer MN, Mencucci MV, Abba M, Lacunza E, Gagliardino JJ Abstract BACKGROUND: Genomic studies facilitate comprehension of the pathophysiology, diagnosis, and treatment of chronic diseases. Such studies require sufficient and good quality DNA isolated from a large number of blood samples. This study...
Tue Nov 12, 2019 17:19
Related ArticlesPlasma MicroRNA-34a as a Potential Biomarker for Early Diagnosis of Esophageal Cancer. Clin Lab. 2019 Nov 01;65(11): Authors: Lin Y, Lin Z, Fang Z, Li H, Zhi X, Zhang Z Abstract BACKGROUND: Esophageal cancer (EC) is one of the cancers with high morbidity and mortality in the world. The dysregulation of microRNAs (miRNAs) would affect the development of cancers, which can be used as potential and non-invasive diagnostic marker. miRNA-34a...
Tue Nov 12, 2019 17:19
Related ArticlesWhich Markers Should the used for Diagnostic Chronic Lymphocytic Leukemia Immunophenotyping Scoring System by Flow Cytometry? Clin Lab. 2019 Nov 01;65(11): Authors: Falay M, Serdar MA, Dalgali H, Uçar MA, Dagdaş S, Özet G Abstract BACKGROUND: The scoring system used for chronic lymphocytic leukemia (CLL) cannot make an accurate diagnosis in some cases. Novel markers are available for the differential diagnosis of CLL, especially from...
Tue Nov 12, 2019 17:19
Related ArticlesCorrelation between miR-103 and miR-133a Expression and the Circulating ANGPTL8 in Type 2 Diabetic Patients and Healthy Control Subjects. Clin Lab. 2019 Nov 01;65(11): Authors: Ghasemi H, Karimi J, Khodadadi I, Tavilani H Abstract BACKGROUND: Angiopoietin-like protein 8 (ANGPTL8) is a circulatory hormone that plays an important role in the proliferation of the pancreatic beta cells and lipid metabolism. MicroRNAs (miRs) are small non-coding...
Tue Nov 12, 2019 17:19
Related ArticlesThe Inspiration from the Final Report from a 46-Year-Old Male Infected by Chlamydia trachomatis and Neisseria gonorrhoeae. Clin Lab. 2019 Nov 01;65(11): Authors: Zheng LQ, Gao Y, Wang QY PMID: 31710445 [PubMed - in process]
Tue Nov 12, 2019 17:19
Related ArticlesCombined use of Serum miR-499a-5p and CA199 Increases the Diagnostic Sensitivity of Pancreatic Cancer. Clin Lab. 2019 Nov 01;65(11): Authors: Shi Q, Feng K, Xia L, Wang C, Zhu J Abstract BACKGROUND: The current study aims to explore the clinical value of serum miR-499a-5p in the diagnosis of pancreatic cancer. METHODS: One hundred twenty-four patients with pancreatic cancer (cancer group), 100 patients with benign pancreatic...
Tue Nov 12, 2019 17:19
Related ArticlesAssociation between Urinary Levels of Interleukin-6, Interleukin-10 and Tumor Necrosis Factor-Alpha with Glomerular and Tubular Damage Indicators in Patients with Type 2 Diabetes. Clin Lab. 2019 Nov 01;65(11): Authors: Sangoi MB, Carvalho JAM, Guarda NS, Duarte T, Duarte MMMF, Premaor MO, Comim FV, Moretto MB, Moresco RN Abstract BACKGROUND: This study investigated the association between urinary levels of interleukin (IL)-6, IL-10, and...
Tue Nov 12, 2019 17:19
Related ArticlesSpurious Low WBC Count from Sysmex XN3000: a Case Report. Clin Lab. 2019 Nov 01;65(11): Authors: Xu C, Yi Y, Zhu D, Xie Z, Li X, Xi Y Abstract BACKGROUND: We present a case of spurious low WBC count in a liver transplant patient. The patient is a 56-year-old man with liver cancer. METHODS: His routine blood test revealed a decrease in WBC count: 0.03 x 109/L compared to 19.30 x 109/L before. The WBCs in the blood smear appeared...
Tue Nov 12, 2019 17:19
Related ArticlesAbnormally Expressed Circular RNAs are Promising Biomarkers for Diagnosis of Hepatocellular Carcinoma: a Meta-Analysis. Clin Lab. 2019 Nov 01;65(11): Authors: Yu G, Yang L, Zhou J, Zhang L, Xia L Abstract BACKGROUND: Circular RNAs (circRNAs), which are expressed aberrantly in tissues and body fluids in individuals with hepatocellular carcinoma (HCC), may serve as promising biomarkers for diagnosis of HCC. We performed a meta-analysis...
Tue Nov 12, 2019 17:19
Related ArticlesAnalytical and Biological Variability of Urinary Epidermal Growth Factor-to-Creatinine Ratio in Patients with Chronic Kidney Disease and in Healthy Volunteers. Clin Lab. 2019 Nov 01;65(11): Authors: Segarra A, Martinez C, Carnicer C, Perich C, Jatem E, Martin M Abstract BACKGROUND: Urinary levels of epidermal growth factor to creatinine ratio are considered as an early predictor of interstitial kidney fibrosis but so far no data are available...
Tue Nov 12, 2019 17:19
Related ArticlesIdentification of lncRNAs Associated with Prognosis of Rectal Cancer by Comprehensive Bioinformatics Analysis Based on TCGA. Clin Lab. 2019 Nov 01;65(11): Authors: Wang C, Yuan G, Chen J Abstract BACKGROUND: Although many advances have been made in the field of molecular biology, new approaches are still required to reveal the molecular mechanisms underlying the pathogenesis of rectal cancer and identify specific diagnostic and therapeutic...
Tue Nov 12, 2019 17:19
Related ArticlesComparison of the New, Rapid, and Fully Automated Kryptor TSH receptor Antibodies Assay (B.R.A.H.M.S.) with the Radioimmunological Assay (B.R.A.H.M.S.). Clin Lab. 2019 Nov 01;65(11): Authors: Chauvet J, Leven C, Thuillier P, Capaldo C, Moineau MP, Plée-Gautier E, Carré JL, Padelli M Abstract BACKGROUND: Radioimmunoassays, which are often not automated and time-consuming, are gradually being re-placed in medical laboratories by non-radioactive...
Tue Nov 12, 2019 17:19
Related ArticlesFunction of Long Noncoding RNA UCA1 on Gastric Cancer Cells and its Clinicopathological Significance in Plasma. Clin Lab. 2019 Nov 01;65(11): Authors: Tang X, Yu L, Bao J, Jiang P, Yan F Abstract BACKGROUND: Diagnosis and treatment of gastric cancer (GC) at early stages is challenging, causing great difficulties to patients. Long noncoding RNAs (lncRNAs) may serve both as novel molecular markers and therapeutic targets in diagnosis and...
Tue Nov 12, 2019 17:19
Related ArticlesThe Association of Long Non-Coding RNA SNHG6 Expression with Clinicopathological Features and Prognosis in Cancer. Clin Lab. 2019 Nov 01;65(11): Authors: Wu Y, Zhang Y, Zhu X, Liu H Abstract BACKGROUND: Although some recent studies have shown that small nucleolar RNA host gene 6 (SNHG6) was associated with clinical outcomes in malignant tumors, the results remain insufficient and inconsistent. Therefore, we performed this meta-analysis...
Tue Nov 12, 2019 17:19
Related ArticlesA Case of Complex Chromosome Translocation: 46, XY, t(4; 10; 13) (q31; q23; q12). Clin Lab. 2019 Nov 01;65(11): Authors: Wang K, Zhao X, Tu H, Lin H Abstract BACKGROUND: Complex chromosome rearrangement (CCR) often results in patients with mental retardation, stunted growth, and multiple abnormalities. CCR carriers are at high risk of adverse pregnancy, and prenatal diagnosis should be made even in normal pregnancy. The incidence of spermatogenesis...
Tue Nov 12, 2019 17:19
Related ArticlesRed Cell and Reticulocyte Parameters for the Detection of Iron Deficiency in Pregnancy. Clin Lab. 2019 Nov 01;65(11): Authors: Crispin PJ, Sethna F, Andriolo K Abstract BACKGROUND: Iron deficiency is a common complication of pregnancy and may lead to anemia as pregnancy progresses. Routine screening tests in pregnancy include hemoglobin levels, but in most centers not a serum ferritin. Advances in red cell and reticulocyte indices on...
Tue Nov 12, 2019 17:19
Related ArticlesA Novel Approach of Urinary Monohydroxyphenyl Metabolites Assay in Cancer Screening. Clin Lab. 2019 Nov 01;65(11): Authors: Gan C, Yao LQ, Su N, Zhu JH, Li F, Li WT, Wang YY, Shi YX, Yu HT Abstract BACKGROUND: A noninvasive, fast, highly sensitive and simple test is needed for cancer screening in addition to the detection of biomarkers in blood. Recently, the patent (CN102565055A) for the Urinary Monohydroxyphenyl Metabolites Assay (UMM-A)...
Tue Nov 12, 2019 17:19
Related ArticlesRare Meningitis and Epileptic Seizure Infected with Brucella melitensis: a Case Report. Clin Lab. 2019 Nov 01;65(11): Authors: Li X, Wang Q, Gong J, Huang F, Feng J, Liang J, Hu B Abstract BACKGROUND: Although brucellosis is the most common zoonotic disease worldwide, human meningitis infected with Brucella melitensis is rare and difficult to diagnosis. Herein we describe the clinical aspects of a rare case of Brucella melitensis meningitis...
Tue Nov 12, 2019 17:19
Related ArticlesSerum Periostin Level is not a Predictor of Early Stage Bone Loss in Chinese Postmenopausal Women. Clin Lab. 2019 Nov 01;65(11): Authors: Luo J, Deng W Abstract BACKGROUND: Postmenopausal osteoporosis (PMOP) is one of the main threats to the health of women, which makes the early detection of bone loss vital. Many studies were carried out dedicated to finding new parameters and improving the discovery rate of early stage bone loss in...
Tue Nov 12, 2019 17:19
Related ArticlesDiagnostic Value of D-dimer in Distinguishing between Spontaneous Subarachnoid Hemorrhage and Nontraumatic Acute Headache. Clin Lab. 2019 Nov 01;65(11): Authors: Zhang Y, Yang P, Wang J Abstract BACKGROUND: Spontaneous subarachnoid hemorrhage (SSAH) and nontraumatic acute headache patients presented with symptoms similar to headache. This study was to investigate the diagnostic value of D-dimer (DD) in distinguishing patients with SSAH...
Tue Nov 12, 2019 17:19
Related ArticlesDiagnostic Significance of miR-937 in Peripheral Blood Mononuclear Cells of Kawasaki Disease. Clin Lab. 2019 Nov 01;65(11): Authors: Wang Z, Zhou J, Dong N, Li W Abstract Beckground: The current study aims to investigate whether miR-937 can be used as a diagnostic biomarker in peripheral blood mononuclear cells (PBMCs) for children with Kawasaki disease (KD) with or without coronary artery dilation (CAD). METHODS: Gene chip technology...
Tue Nov 12, 2019 17:19
Related ArticlesComparison and Evaluation of Lowenstein-Jensen Medium, Mycobacteria Growth Indicator Tube 960, and Microscopic Smear Results in the Diagnosis of Tuberculosis: Insights from a Large, Retrospective, Single Centre Study. Clin Lab. 2019 Nov 01;65(11): Authors: Yazisiz H, Cenger DH, Yazisiz V, Kiliç L, Altin S PMID: 31710428 [PubMed - in process]
Tue Nov 12, 2019 17:19
Related ArticlesEffect of CYP3A5 and ABCB1 Gene Polymorphisms on Tacrolimus Blood Concentration in Renal Transplant Recipients. Clin Lab. 2019 Nov 01;65(11): Authors: Yildirim E, Şahin G, Kaltuş Z, Çolak E Abstract BACKGROUND: Tacrolimus (Tac) is a calcineurin inhibitor (CNI). Its therapeutic range is narrow and pharmacokinetic properties vary among patients. CYP3A5 and MDR1 single-nucleotide polymorphisms (SNPs) are the most effective polymorphisms...
Tue Nov 12, 2019 17:19
Related ArticlesReference Intervals for Serum Creatinine with Jaffe's Method in Healthy Chinese Geriatric Population. Clin Lab. 2019 Nov 01;65(11): Authors: Wang Y, Yang Y, Hu M, Tang A, Xiang Z Abstract BACKGROUND: Reliable reference intervals (RIs) for the serum creatinine (SCr), assayed by Jaffe's method and categorized in small intervals, are still lacking in elderly population. The study aims to establish RIs for SCr with Jaffe's method in healthy...
Tue Nov 12, 2019 17:19
Related ArticlesDistinguishing Four HCV Genotypes by Loop Mediated Isothermal Amplification Assay. Clin Lab. 2019 Nov 01;65(11): Authors: Yang C, Li X, Li H, Guo C, Li Y, Liu Z Abstract BACKGROUND: Genotyping of hepatitis C virus (HCV) is of great significance for clinical diagnosis and treatment. The object of this work was to establish a loop-mediated isothermal amplification (LAMP) method for four subtypes of HCV prevalent in China. METHODS:...
Tue Nov 12, 2019 17:19
Related ArticlesEvaluation of PAP in EGFR Mutational Testing in Advanced NSCLC: a Comparative Study. Clin Lab. 2019 Nov 01;65(11): Authors: Jinliang C, Yang X, Xiaoyan X, Yudong S, Xinyue W, Zhaoting M, Li L, Ning S, Hua G, Dingzhi H, Lun Z, Peng C Abstract BACKGROUND: EGFR mutational testing is crucial for advanced non-squamous NSCLC. PAP is a sensitive and selective method to detect rare mutations. METHODS: Eighty-five patients with non-squamous...
Tue Nov 12, 2019 17:19
Related ArticlesRecurrent upper limb neuropathies secondary to an epithelioid haemangioendothelioma - A rare mimic of nerve tumours. J Clin Neurosci. 2019 Nov 07;: Authors: Triplett JD, Khor TS, Kermode AG Abstract Epithelioid haemangioendothelioma (EHE) is an uncommon type of vascular tumour with intermediate malignant potential, classified as a sarcoma which occasionally involves neurovascular structures and can mimic nerve sheath tumours. EHE is difficult...
Tue Nov 12, 2019 13:23
Related ArticlesPhotogrammetric analysis: An objective measure to assess the craniocervical range of motion after cervical laminoplasty surgeries. J Clin Neurosci. 2019 Nov 07;: Authors: Janjua MB, Zhou PL, Vasquez-Montes D, Moskovich R Abstract Surgical decompression using laminoplasty is commonly performed for multilevel stenosis with cervical spondylotic myelopathy. However, the long-term effects on the craniocervical range of motion (ROM) after surgery...
Tue Nov 12, 2019 13:23
Related ArticlesDetermination of the electrical impedance of neural tissue from its microscopic cellular constituents. J Neural Eng. 2019 Nov 11;: Authors: Monfared O, Tahayori B, Freestone D, Nesic D, Grayden DB, Meffin H Abstract The electrical properties of neural tissue are important in a range of dierent applications in biomedical engineering and basic science. These properties are characterized by the electrical admittivity of the tissue, which...
Tue Nov 12, 2019 16:16
Related ArticlesAn Experimental Paradigm for Measuring the Effects of Ageing on Sentence Processing. J Vis Exp. 2019 Oct 25;(152): Authors: Liu X, Wang H, Xie A, Mao X Abstract Previous studies have found that older adults have greater difficulties in processing syntactically complex sentences than younger adults. However, the exact regions where the difficulties arise have not been fully identified. In this study, a maze task was implemented to investigate...
Tue Nov 12, 2019 16:33
Related ArticlesSilencing the Spark: CRISPR/Cas9 Genome Editing in Weakly Electric Fish. J Vis Exp. 2019 Oct 27;(152): Authors: Constantinou SJ, Nguyen L, Kirschbaum F, Salazar VL, Gallant JR Abstract Electroreception and electrogenesis have changed in the evolutionary history of vertebrates. There is a striking degree of convergence in these independently derived phenotypes, which share a common genetic architecture. This is perhaps best exemplified...
Tue Nov 12, 2019 16:33
Related ArticlesProstate Organoid Cultures as Tools to Translate Genotypes and Mutational Profiles to Pharmacological Responses. J Vis Exp. 2019 Oct 24;(152): Authors: Pappas KJ, Choi D, Sawyers CL, Karthaus WR Abstract Presented here is a protocol to study pharmacodynamics, stem cell potential, and cancer differentiation in prostate epithelial organoids. Prostate organoids are androgen responsive, three-dimensional (3D) cultures grown in a defined medium...
Tue Nov 12, 2019 16:33
Related ArticlesRadiotracer Administration for High Temporal Resolution Positron Emission Tomography of the Human Brain: Application to FDG-fPET. J Vis Exp. 2019 Oct 22;(152): Authors: Jamadar SD, Ward PGD, Carey A, McIntyre R, Parkes L, Sasan D, Fallon J, Orchard E, Li S, Chen Z, Egan GF Abstract Functional positron emission tomography (fPET) provides a method to track molecular targets in the human brain. With a radioactively-labelled glucose analogue,...
Tue Nov 12, 2019 16:33
Related ArticlesBulk Droplet Vitrification for Primary Hepatocyte Preservation. J Vis Exp. 2019 Oct 25;(152): Authors: de Vries RJ, Banik PD, Nagpal S, Weng L, Ozer S, van Gulik TM, Toner M, Tessier SN, Uygun K Abstract Vitrification is a promising ice-free alternative for classic slow-freezing (at approximately 1 °C/min) cryopreservation of biological samples. Vitrification requires extremely fast cooling rates to achieve transition of water into the...
Tue Nov 12, 2019 16:33
Related ArticlesSolid Phase 11C-Methylation, Purification and Formulation for the Production of PET Tracers. J Vis Exp. 2019 Oct 24;(152): Authors: Singleton TA, Boudjemeline M, Hopewell R, Jolly D, Bdair H, Kostikov A Abstract Routine production of radiotracers used in positron emission tomography (PET) mostly relies on wet chemistry where the radioactive synthon reacts with a non-radioactive precursor in solution. This approach necessitates purification...
Tue Nov 12, 2019 16:33
Related ArticlesAbsolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry. J Vis Exp. 2019 Oct 25;(152): Authors: Vilkhovoy M, Dai D, Vadhin S, Adhikari A, Varner JD Abstract Cell-free protein synthesis (CFPS) is an emerging technology in systems and synthetic biology for the in vitro production of proteins. However, if CFPS is going to move beyond the laboratory and become a widespread...
Tue Nov 12, 2019 16:33
Related ArticlesExperimental Approach to Examine Leptin Signaling in the Carotid Bodies and its Effects on Control of Breathing. J Vis Exp. 2019 Oct 25;(152): Authors: Shin MK, Kim LJ, Caballero-Eraso C, Polotsky VY Abstract An adipocyte-produced hormone leptin is a potent respiratory stimulant, which may play an important role in defending respiratory function in obesity. The carotid bodies (CB), a key organ of peripheral hypoxic sensitivity, express...
Tue Nov 12, 2019 16:33
Related Articles3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography. J Vis Exp. 2019 Oct 24;(152): Authors: Busse M, Müller M, Kimm MA, Ferstl S, Allner S, Achterhold K, Herzen J, Pfeiffer F Abstract We demonstrate a laboratory-based method combining X-ray microCT and nanoCT with a specific X-ray stain, which targets the cell cytoplasm. The described protocol is easy to apply, fast and suitable...
Tue Nov 12, 2019 16:33
Related ArticlesSubcellular Fractionation of Primary Chronic Lymphocytic Leukemia Cells to Monitor Nuclear/Cytoplasmic Protein Trafficking. J Vis Exp. 2019 Oct 23;(152): Authors: Hay J, Moles MW, Cassels J, Michie AM Abstract Nuclear export of macromolecules is often deregulated in cancer cells. Tumor suppressor proteins, such as p53, can be rendered inactive due to aberrant cellular localization disrupting their mechanism of action. The survival of...
Tue Nov 12, 2019 16:33
Related ArticlesClonal Genetic Tracing using the Confetti Mouse to Study Mineralized Tissues. J Vis Exp. 2019 Oct 23;(152): Authors: Zhou B, Kaucka M, Chagin AS, Newton PT Abstract Labeling an individual cell in the body to monitor which cell types it can give rise to and track its migration through the organism or determine its longevity can be a powerful way to reveal mechanisms of tissue development and maintenance. One of the most important tools...
Tue Nov 12, 2019 16:33
Related ArticlesInvasive Hemodynamic Assessment for the Right Ventricular System and Hypoxia-Induced Pulmonary Arterial Hypertension in Mice. J Vis Exp. 2019 Oct 24;(152): Authors: Luo F, Wang X, Luo X, Li B, Zhu D, Sun H, Tang Y Abstract Pulmonary arterial hypertension (PAH) is a chronic and severe cardiopulmonary disorder. Mice are a popular animal model used to mimic this disease. However, the evaluation of right ventricular pressure (RVP) and pulmonary...
Tue Nov 12, 2019 16:33
Related ArticlesIn Vivo Assessment of Alveolar Macrophage Efferocytosis Following Ozone Exposure. J Vis Exp. 2019 Oct 22;(152): Authors: Hodge MX, Reece SW, Madenspacher JH, Gowdy KM Abstract Ozone (O3) is a criteria air pollutant that exacerbates and increases the incidence of chronic pulmonary diseases. O3 exposure is known to induce pulmonary inflammation, but little is known regarding how exposure alters processes important to the resolution of inflammation....
Tue Nov 12, 2019 16:33
Related ArticlesIdentification of Host Pathways Targeted by Bacterial Effector Proteins using Yeast Toxicity and Suppressor Screens. J Vis Exp. 2019 Oct 25;(152): Authors: Faris R, Weber MM Abstract Intracellular bacteria secrete virulence factors called effector proteins into the host cytosol that act to subvert host proteins and/or their associated biological pathways to the benefit of the bacterium. Identification of putative bacterial effector proteins...
Tue Nov 12, 2019 16:33
Related ArticlesLaser-Induced Fluorescence Emission (L.I.F.E.) as Novel Non-Invasive Tool for In-Situ Measurements of Biomarkers in Cryospheric Habitats. J Vis Exp. 2019 Oct 26;(152): Authors: Weisleitner K, Hunger L, Kohstall C, Frisch A, Storrie-Lombardi MC, Sattler B Abstract Global warming affects microbial communities in a variety of ecosystems, especially cryospheric habitats. However, little is known about microbial-mediated carbon fluxes in extreme...
Tue Nov 12, 2019 16:33
Related ArticlesImmunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain. J Vis Exp. 2019 Oct 27;(152): Authors: González Ibanez F, Picard K, Bordelau M, Sharma K, Bisht K, Tremblay MÈ Abstract This is a protocol for the dual visualization of microglia and infiltrating macrophages in mouse brain tissue. TMEM119 (which labels microglia selectively), when combined...
Tue Nov 12, 2019 16:33
Related ArticlesSingle Cell Micro-aspiration as an Alternative Strategy to Fluorescence-activated Cell Sorting for Giant Virus Mixture Separation. J Vis Exp. 2019 Oct 27;(152): Authors: Sahmi-Bounsiar D, Boratto PVM, Oliveira GP, Bou Khalil JY, La Scola B, Andreani J Abstract During the amoeba co-culture process, more than one virus may be isolated in a single well. We previously solved this issue by end point dilution and/or fluorescence activated cell...
Tue Nov 12, 2019 16:33
Related ArticlesPrimary Culture of Neurons Isolated from Embryonic Mouse Cerebellum. J Vis Exp. 2019 Oct 26;(152): Authors: Shabanipour S, Dalvand A, Jiao X, Rahimi Balaei M, Chung SH, Kong J, Del Bigio MR, Marzban H Abstract The use of primary cell cultures has become one of the major tools to study the nervous system in vitro. The ultimate goal of using this simplified model system is to provide a controlled microenvironment and maintain the high survival...
Tue Nov 12, 2019 16:33
Related ArticlesA Training and Testing System for Performing Vascular Reconstruction In Vitro. J Vis Exp. 2019 Oct 26;(152): Authors: Wang Y, Mu L, Zhang W, Chen H, Li Q, Shi A, Tang B, Zhang X, Dong D, Lv Y Abstract Manual vascular reconstruction training is essential for a beginner surgeon. However, an optimal training system for vascular reconstruction in vitro has yet to be developed. In this study, we introduce an in vitro training and testing system...
Tue Nov 12, 2019 16:33
Related ArticlessiRNA Electroporation to Modulate Autophagy in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells. J Vis Exp. 2019 Oct 28;(152): Authors: Düthorn A, Turan A, Draßner C, Mühl-Zürbes P, Heilingloh CS, Steinkasserer A, Grosche L Abstract Herpes simplex virus type-1 (HSV-1) induces autophagy in both, immature dendritic cells (iDCs) as well as mature dendritic cells (mDCs), whereas autophagic flux is only observed in iDCs....
Tue Nov 12, 2019 16:33
Related ArticlesHow to Obtain Reliable Visual Event-related Potentials in Newborns. J Vis Exp. 2019 Oct 24;(152): Authors: Cubero-Rego L, Ricardo-Garcell J, Harmony T, Corsi-Cabrera M Abstract The present study discusses the characteristics of visual event-related potentials (VEPs) and outlines methodological steps for obtaining reliable measurements in newborns. Obtaining high-quality, reliable VEPs is crucial for the early detection of abnormal development...
Tue Nov 12, 2019 16:33
Related ArticlesSimple Surgical Induction of Conductive Hearing Loss with Verification Using Otoscope Visualization and Behavioral Clap Startle Response in Rat. J Vis Exp. 2019 Oct 26;(152): Authors: Manno FAM, Manno SHC, Ma V, Barrios FA, Cho WC, Cheng SH, Lau C Abstract Conductive hearing loss (CHL) is a prevalent hearing impairment in humans. The goal of the protocol is to describe a simple surgical procedure for inducing CHL in rodents. The protocol...
Tue Nov 12, 2019 16:33
Related ArticlesUsing Human Induced Pluripotent Stem Cells for the Generation of Tumor Antigen-specific T Cells. J Vis Exp. 2019 Oct 24;(152): Authors: Good ML, Vizcardo R, Maeda T, Tamaoki N, Malekzadeh P, Kawamoto H, Restifo NP Abstract The generation and expansion of functional T cells in vitro can lead to a broad range of clinical applications. One such use is for the treatment of patients with advanced cancer. Adoptive T cell transfer (ACT) of highly...
Tue Nov 12, 2019 16:33
Related ArticlesKinase Inhibitor Screening In Self-assembled Human Protein Microarrays. J Vis Exp. 2019 Oct 23;(152): Authors: Festa F, Labaer J Abstract The screening of kinase inhibitors is crucial for better understanding properties of a drug and for the identification of potentially new targets with clinical implications. Several methodologies have been reported to accomplish such screening. However, each has its own limitations (e.g., the screening...
Tue Nov 12, 2019 16:33
Related ArticlesTissue Collection of Bats for -Omics Analyses and Primary Cell Culture. J Vis Exp. 2019 Oct 23;(152): Authors: Yohe LR, Devanna P, Davies KTJ, Potter JHT, Rossiter SJ, Teeling EC, Vernes SC, Dávalos LM Abstract As high-throughput sequencing technologies advance, standardized methods for high quality tissue acquisition and preservation allow for the extension of these methods to non-model organisms. A series of protocols to optimize tissue...
Tue Nov 12, 2019 16:33
Related ArticlesLaminectomy and Spinal Cord Window Implantation in the Mouse. J Vis Exp. 2019 Oct 23;(152): Authors: Pietruczyk EA, Stephen TKL, Alford S, Lutz SE Abstract This protocol describes a method for spinal cord laminectomy and glass window implantation for in vivo imaging of the mouse spinal cord. An integrated digital vaporizer is utilized to achieve a stable plane of anesthesia at a low-flow rate of isoflurane. A single vertebral spine is...
Tue Nov 12, 2019 16:33
Related ArticlesAtom Probe Tomography Analysis of Exsolved Mineral Phases. J Vis Exp. 2019 Oct 25;(152): Authors: Genareau K, Perez-Huerta A, Laiginhas F Abstract Element diffusion rates and temperature/pressure control a range of fundamental volcanic and metamorphic processes. Such processes are often recorded in lamellae exsolved from host mineral phases. Thus, the analysis of the orientation, size, morphology, composition and spacing of exsolution...
Tue Nov 12, 2019 16:33
Related ArticlesComparing Bibliometric Analysis Using PubMed, Scopus, and Web of Science Databases. J Vis Exp. 2019 Oct 24;(152): Authors: AlRyalat SAS, Malkawi LW, Momani SM Abstract Literature databases (i.e., PubMed, Scopus, and Web of Science) differ in terms of their coverage, focus, and the tool they provide. PubMed focuses mainly on life sciences and biomedical disciplines, whereas Scopus and Web of Science are multidisciplinary. The protocol...
Tue Nov 12, 2019 16:33
Related ArticlesSevere late dysphagia after multimodal treatment of stage III/IV laryngeal and hypopharyngeal cancer. Jpn J Clin Oncol. 2019 Nov 11;: Authors: Huh G, Ahn SH, Suk JG, Lee MH, Kim WS, Kwon SK, Ock CY, Keam B, Heo DS, Kim JH, Wu HG Abstract BACKGROUND: Long-term side effects after radiotherapy for organ preservation 'could deteriorate' the laryngeal function. This study intended to identify the incidence of severe late dysphagia following...
Tue Nov 12, 2019 16:29
Related ArticlesIn-home auditory training using audiovisual stimuli on a tablet computer: Feasibility and preliminary results. Auris Nasus Larynx. 2019 Nov 07;: Authors: Sato T, Yabushita T, Sakamoto S, Katori Y, Kawase T Abstract OBJECTIVE: To examine the feasibility and possible effects of in-home auditory training using audiovisual speech stimuli on a tablet computer for patients with hearing loss using a hearing aid (HA) or a cochlear implant (CI)....
Tue Nov 12, 2019 14:30
Related ArticlesRetention of cricothyrotomy skills by paramedics using a wire guided technique. Am J Emerg Med. 2019 03;37(3):407-410 Authors: March JA, Kiemeney MJ, De Guzman J, Ferguson JD Abstract INTRODUCTION: Cricothyrotomy may be necessary for airway management when a patient's airway cannot be maintained through standard techniques such as oral airway placement, blind insertion airway device, or endotracheal intubation. Wire-guided cricothyrotomy...
Tue Nov 12, 2019 14:30
Related ArticlesLaryngeal Dysfunction in Cough Hypersensitivity Syndrome: A Cross-Sectional Observational Study. J Allergy Clin Immunol Pract. 2018 Nov - Dec;6(6):2087-2095 Authors: Vertigan AE, Kapela SM, Kearney EK, Gibson PG Abstract BACKGROUND: Chronic refractory cough (CRC), a phenotype of cough hypersensitivity syndrome (CHS), is a disabling problem. Laryngeal dysfunction may be important in CRC and CHS because laryngeal symptoms are common; however,...
Tue Nov 12, 2019 14:30
Related ArticlesUnusual Clinical Manifestation of Laryngeal Edema in a Case of DRESS Syndrome. J Allergy Clin Immunol Pract. 2018 Nov - Dec;6(6):2108-2109 Authors: Sim DW, Koh YI PMID: 29574127 [PubMed - indexed for MEDLINE]
Tue Nov 12, 2019 14:30
Related ArticlesComparison of two fetoscopic open neural tube defect (ONTD) repair techniques: Single-layer vs three-layer closure. Ultrasound Obstet Gynecol. 2019 Nov 11;: Authors: Belfort MA, Whitehead WE, Shamshirsaz AA, Espinoza J, Nassr AA, Lee TC, Olutoye OO, Keswani SG, Cortes MS Abstract OBJECTIVES: We previously reported on a fetoscopic repair for open neural tube defect (ONTD) using a single layer closure (SLC). Because of a high rate of cerebrospinal...
Tue Nov 12, 2019 13:29
Related ArticlesEndoscopic endonasal versus transcranial approach to resection of olfactory groove meningiomas: a systematic review. Neurosurg Rev. 2019 Nov 10;: Authors: Purohit A, Jha R, Khalafallah AM, Price C, Rowan NR, Mukherjee D Abstract Despite the increasing utility of the endoscopic endonasal approach (EEA) for management of anterior skull base (ASB) pathologies, the optimal treatment strategy for olfactory groove meningiomas (OGM) remains...
Tue Nov 12, 2019 13:29
Related ArticlesFrequency analyses of posturography using logarithmic translation. Acta Otolaryngol. 2019 Nov 09;:1-7 Authors: Watanabe H, Makabe A, Hirai C, Takeda T, Honda K, Demura S, Tsutsumi T Abstract Background: Power-spectral analysis of the centre of pressure (CoP) frequencies of posturography provides exponentially approximated distributions, whereas logarithmic translation enables linear approximation.Objectives: Frequency analyses were adopted...
Tue Nov 12, 2019 16:04
Related ArticlesTranscanal endoscopic ear surgery for traumatic ossicular injury. Acta Otolaryngol. 2019 Nov 09;:1-5 Authors: Kim MS, Chung J, Kang JY, Choi JW Abstract Background: Transcanal endoscopic ear surgery (TEES) for traumatic ossicular injuries (TOI) is not well studied.Objectives: To analyze the treatment outcomes of TEES for TOI.Material and methods: Retrospective case review of fifteen patients (15 ears) with TOI was performed. The patients...
Tue Nov 12, 2019 13:04
Related ArticlesPresentation and Management of Killian Jamieson Diverticulum: A Comprehensive Literature Review. Ann Otol Rhinol Laryngol. 2019 Nov 09;:3489419887403 Authors: Haddad N, Agarwal P, Levi JN, Tracy JC, Tracy LF Abstract OBJECTIVE: To examine the clinical presentation, diagnostic evaluation, and management of Killian-Jamieson diverticula (KJD) through literature review. METHODS: A comprehensive literature review was conducted through...
Tue Nov 12, 2019 13:37
Related ArticlesValue-based pricing: Toward achieving a balance between individual and population gains in health benefits. Cancer Med. 2019 Nov 11;: Authors: Parmar A, Jiao T, Saluja R, Chan KKW Abstract OBJECTIVES: Value-based pricing of oncology drugs provides a best estimate for the price of a drug, as it relates to the benefits it provides for individual patients. To date, the impact of value-based pricing to reference cost-effectiveness thresholds...
Tue Nov 12, 2019 17:48
Related ArticlesAnti-tumor effects of an antagonistic mAb against the ASCT2 amino acid transporter on KRAS-mutated human colorectal cancer cells. Cancer Med. 2019 Nov 10;: Authors: Hara Y, Minami Y, Yoshimoto S, Hayashi N, Yamasaki A, Ueda S, Masuko K, Masuko T Abstract KRAS mutations are detected in numerous human cancers, but there are few effective drugs for KRAS-mutated cancers. Transporters for amino acids and glucose are highly expressed on cancer...
Tue Nov 12, 2019 13:53
Related ArticlesUnbiased enrichment of urine exfoliated cells on nanostructured substrates for sensitive detection of urothelial tumor cells. Cancer Med. 2019 Nov 10;: Authors: Wang X, Gu Y, Zhang S, Li G, Liu T, Wang T, Qin H, Jiang B, Zhu L, Li Y, Lei H, Li M, Zhang Q, Yang R, Fang F, Guo H Abstract BACKGROUND: Early detection of urothelial carcinoma (UC) by noninvasive diagnostic methods with high accuracy is still underscored. This study aimed to...
Tue Nov 12, 2019 13:53
Related ArticlesThe prevalence and prognostic value of KRAS co-mutation subtypes in Chinese advanced non-small cell lung cancer patients. Cancer Med. 2019 Nov 10;: Authors: Cai D, Hu C, Li L, Deng S, Yang J, Han-Zhang H, Li M Abstract OBJECTIVE: KRAS mutation plays a critical role in the initiation and development of non-small cell lung cancer (NSCLC). KRAS-mutant patients exhibit diverse response to chemotherapy. KRAS co-mutation subtypes and their...
Tue Nov 12, 2019 13:53
Related ArticlesEliciting dose is associated with tolerance development in peanut and cow's milk allergic children. Clin Transl Allergy. 2019;9:58 Authors: Nitsche C, Westerlaken-van Ginkel CD, Kollen BJ, Sprikkelman AB, Koppelman GH, Dubois AEJ Abstract Background: Tolerance development rates differ between food allergies. Almost all previous studies have not used the gold standard method, the double-blind, placebo-controlled food challenge (DBPCFC),...
Tue Nov 12, 2019 14:51
Related ArticlesAccuracy of computer-assisted image analysis in the diagnosis of maxillofacial radiolucent lesions: A systematic review and meta-analysis. Dentomaxillofac Radiol. 2019 Nov 11;:20190204 Authors: Silva VKS, Vieira WA, Bernardino IM, Travençolo BAN, Bittencourt MAV, Blumenberg C, Paranhos LR, Galvão HC Abstract OBJECTIVES: This study aimed to search for scientific evidence concerning the accuracy of computer-assisted analysis for diagnosing...
Tue Nov 12, 2019 19:05
Related ArticlesValidity of micro-CT for in vitro caries detection: a systematic review and meta-analysis. Dentomaxillofac Radiol. 2019 Nov 11;:20190347 Authors: Oliveira LB, Massignan C, Oenning AC, Rovaris K, Bolan M, Porporatti AL, De Luca Canto G Abstract OBJECTIVE: To investigate the validity of micro-CT for in vitro caries detection in comparison with histology as the reference standard. METHODS: A systematic search was conducted in the...
Tue Nov 12, 2019 19:05
Related ArticlesIs axillary lymph node dissection necessary for positive preoperative aspiration cytology lymph node results? Eur J Surg Oncol. 2019 Nov 01;: Authors: Horváth Z, Paszt A, Simonka Z, Látos M, Kaizer L, Hamar S, Vörös A, Ormándi K, Fejes Z, Lázár G Abstract INTRODUCTION: Based on international guidelines, axillary lymph node dissection (ALND) is recommended in cases of breast cancer if preoperative examinations confirm axillary metastasis....
Tue Nov 12, 2019 14:48
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