Abstract Background Bilateral thalamic astrocytomas in children are exceedingly rare. These highly malignant tumors seldom respond to conventional treatment strategies and carry a grim prognosis for patients. However, recent advances in molecular oncology have had a positive impact on prognostication and treatment strategies of these tumors. Case-based review ...
Sat Sep 14, 2019 03:00
We present a protocol to measure the magnetic field dependence of the spin-lattice relaxation time of 13C-enriched compounds, hyperpolarized by means of dynamic nuclear polarization, using fast field-cycled relaxometry. Specifically, we have demonstrated this with [1-13C]pyruvate, but the protocol could be extended to other hyperpolarized substrates.
Fri Sep 13, 2019 20:00
This article provides detailed methods for fabricating and characterizing a pneumatically actuating microfluidic device for chondrocyte compression.
Fri Sep 13, 2019 20:15
This protocol describes a reproducible method for isolation of mouse rhabdomyosarcoma primary cells, tumorsphere formation and treatment, and allograft transplantation starting from tumorspheres cultures.
Fri Sep 13, 2019 21:00
Presented here is a protocol for a single-cell, epifluorescence microscopy-based technique to quantify grazing rates in aquatic predatory eukaryotes with high precision and taxonomic resolution.
Fri Sep 13, 2019 21:17
The aim of the presented protocol is to investigate the role of visual imagery in the bouba/kiki-effect, whether training in noticing the bouba/kiki shape-audio regularities affects the bouba/kiki-effect and the recognition of individual bouba and kiki shapes, and finally what mental images these regularities produce.
Fri Sep 13, 2019 22:00
Presented here is an electroporation method for plasmid DNA delivery and ependymoglial cell labeling in the adult zebrafish telencephalon. This protocol is a quick and efficient method to visualize and trace individual ependymoglial cells and opens up new possibilities to apply electroporation to a broad range of genetic manipulations.
Fri Sep 13, 2019 22:18
Here, we present a Cas9-based exon23 deletion protocol to restore dystrophin expression in iPSC from Dmdmdx mouse-derived skin fibroblasts and directly differentiate iPSCs into myogenic progenitor cells (MPC) using the Tet-on MyoD activation system.
Sat Sep 14, 2019 19:34
We describe protocols to prepare oocysts and purify sporozoites for studying infection of human intestinal and airway organoids by Cryptosporidium parvum. We demonstrate the procedures for microinjection of parasites into the intestinal organoid lumen and immunostaining of organoids. Finally, we describe the isolation of generated oocysts from the organoids.
Sat Sep 14, 2019 20:35
Described here is a simple method for the purification of a gene product in Streptococcus mutans. This technique may be advantageous in the purification of proteins, especially membrane proteins and high molecular mass proteins, and can be used with various other bacterial species.
Sat Sep 14, 2019 21:35
Astrocytes are morphologically complex cells, exemplified by their multiple processes and bushy territories. To analyze their elaborate morphology, we present a reliable protocol to perform intracellular Lucifer yellow iontophoresis in lightly fixed tissue.
Sat Sep 14, 2019 22:37
Occlusion pressure at 100 ms (P0.1), defined as the negative pressure measured 100 ms after the initiation of an inspiratory effort performed against a closed respiratory circuit, has been shown to be well cor...
Fri Sep 13, 2019 03:00
Abstract Enhanced external counterpulsation (EECP) is a noninvasive treatment method for coronary artery atherosclerosis that acts on the vascular endothelial cells. The intracoronary hemodynamic parameters that influence long-term treatment effect are the fundamental factors for the inhibition of intimal hyperplasia, which cannot be measured in real time. In order to optimize the long-term treatment effect of coronary heart disease, it is necessary to establish a method for quantified...
Sat Sep 14, 2019 03:00
Abstract For Brain-Computer interfaces (BCIs), system calibration is a lengthy but necessary process for successful operation. Co-adaptive BCIs aim to shorten training and imply positive motivation to users by presenting feedback already at early stages: After just 5 min of gathering calibration data, the systems are able to provide feedback and engage users in a mutual learning process. In this work, we investigate whether the retraining stage of co-adaptive BCIs can be adapted...
Sat Sep 14, 2019 03:00
Objective To demonstrate the safety and compatibility of microvascular couplers in patients undergoing postoperative magnetic resonance imaging (MRI) scans. Study Design Retrospective case series. Methods A consecutive review of 1,252 patients undergoing free tissue transfer for head and neck reconstruction at Oregon Health and Sciences University (OHSU) between 2010 and 2017 who had microvascular coupler Synovis, Baxter Deerfield, IL (Synovis Life Technologies, Saint Paul, MN) implantation...
Fri Sep 13, 2019 18:13
Objectives Osteomas are osseous tumors that primarily occur at the skull, in particular the paranasal sinus system. Surgical tumor resection is the “gold standard” treatment in symptomatic osteomas. The aim of this study was to investigate the use of surgical approaches (endoscopic, open, combined) and to provide a step‐by‐step approach for patients’ management. Methods Fifty‐eight patients (31 m, 27 f, 42.1 ± 15.3 years) that were treated between 2001 and 2015 at our department were included...
Sat Sep 14, 2019 12:39
Objective/Hypothesis The ADHERE Registry is a multicenter prospective observational study following outcomes of upper airway stimulation (UAS) therapy in patients who have failed continuous positive airway pressure therapy for obstructive sleep apnea (OSA). The aim of this registry and purpose of this article were to examine the outcomes of patients receiving UAS for treatment of OSA. Study Design Cohort Study. Methods Demographic and sleep study data collection occurred at baseline,...
Sat Sep 14, 2019 12:52
A fluorescence photomicrograph (ultramicroscopy) demonstrating cerebral blood vessels (red) and Abeta amyloid (green) in the cerebral cortex of a transgenic mouse with the APPDutch mutation in the amyloid precursor protein. Vascular Abeta deposition started in large superficial arteries with few affected small penetrating vessels. Early treatment with beta‐site amyloid precursor protein‐cleaving enzyme 1 (BACE1) inhibitor prevented progression. See Schelle et al., pages 561–571, in this issue. ANN...
Sat Sep 14, 2019 13:28
Qualitative work has described the differences in prescribing practice across medical and surgical specialties. This study aimed to understand if specialty impacts quantitative measures of prescribing practice.
Fri Sep 13, 2019 03:00
Intensity modulated radiotherapy (IMRT) and volumetric modulated arc therapy (VMAT) treatment plan generation is a complex process, traditionally performed manually by medical physicists or specialist dosimetrists. Manual methods can be time consuming and dependent on the treatment planner’s experience [1]. A solution to this problem is automated planning, where high quality plans are generated autonomously with minimal operator interaction [2–9].
Fri Sep 13, 2019 03:00
Approximately 170,000 patients are diagnosed with brain metastases in the United States annually [1]. Nearly 50% of these patients are now treated with stereotactic radiosurgery (SRS) due to preserved cognition and quality of life compared to whole brain radiotherapy (WBRT) [2,3]. Additionally, survival has improved due to more efficacious systemic therapies in the metastatic setting [4]. With this improved survival and control of extracranial disease comes an increased likelihood of distant brain...
Fri Sep 13, 2019 03:00
Bone metastases frequently occur in metastatic cancer patients and the spine is a common site of tumor spread [1]. Palliative radiation therapy (RT) is an effective therapy for painful spinal metastases [2]. Stereotactic body radiation therapy (SBRT) to the spine has been implemented in the last decade as a highly efficient approach [3,4]. By multimodality imaging for target volume definition, modern intensity-modulated treatment planning, radiotherapy delivery with image-guidance and nearly rigid...
Fri Sep 13, 2019 03:00
A common treatment option for breast cancer patients is accelerated partial breast irradiation (APBI) often applied in form of high dose-rate (HDR) multi-catheter interstitial brachytherapy (iBT). The therapy outcome proved to be comparable to other treatment options, such as whole breast irradiation or a percutaneous APBI treatment, with regards to local recurrence rate, disease free survival, overall survival, quality of life, late side effects, toxicity and cosmetic outcome [1–8]. APBI using multi-catheter...
Sat Sep 14, 2019 03:00
Maxillary hypoplasia in cleft lip and palate is a complex deformity. Despite surgical improvements, postoperative relapse persists. This systematic review was performed to determine the mean horizontal relapse rates for the surgical techniques used to treat maxillary hypoplasia: Le Fort I osteotomy with rigid fixation, Le Fort I distraction osteogenesis, and anterior maxillary distraction osteogenesis. This study followed the PRISMA statement. The PubMed, Embase, Science Direct, and Web of Science...
Sat Sep 14, 2019 03:00
The Dysphonia Severity Index (DSI) is an objective multiparameter index of voice quality that measures and describes overall voice quality. Some studies have suggested that the reliability of devices for DSI measurement should be examined. We explored the feasibility of DSI measurements using the Dr. Speech (DRS) device, verified its effectiveness for clinical voice measurements and intradevice reliability, and examined the correlation between the DSI and self-evaluations of voice problems.
Fri Sep 13, 2019 03:00
The primary aim of this research was to quantify the degree to which the upper airway temperature changes with singing warm-up. Based on prior upper airway thermoregulation research it was hypothesized that upper airway temperature would not significantly increase during singing warm up when compared to prewarm up and recovery phases. Ten participants completed a short singing warm-up of their choice until they felt sufficiently warmed up while upper airway temperature was measured at 1 second intervals...
Fri Sep 13, 2019 03:00
In addition to language differences in fundamental frequency between bilinguals and monolinguals, studies have also included other acoustic parameters to analyze differences in voice production associated with the language spoken.
Sat Sep 14, 2019 03:00
The goals of the study were to (a) examine vocal fatigue in speech-language pathology students through subjective and objective measures following a novel 30-minute vocal loading task (VLT) and (b) evaluate the effects of psychosocial factors on vocal fatigue.
Sat Sep 14, 2019 03:00
Thu Sep 12, 2019 20:34
Tue Sep 10, 2019 15:58
Cover Image © Andrey Prokhorov/iStockphoto.
Tue Sep 10, 2019 15:58
Abstract Hartnup disease is an autosomal recessive condition characterized by neutral aminoaciduria and behavioral problems. It is caused by a loss of B0AT1, a neutral amino acid transporter in the kidney and intestine. CLTRN encodes the protein collectrin that functions in the transportation and activation of B0AT1 in the renal apical brush bordered epithelium. Collectrin deficient mice have severe aminoaciduria. However, the phenotype associated with collectrin deficiency in humans has not been...
Fri Sep 13, 2019 19:57
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