Neutrophil extracellular traps (NETs) are three-dimensional structures generated by stimulated neutrophil granulocytes. It has become clear in recent years that NETs are involved in a wide variety of diseases. Detection of NETs in tissue may have diagnostic relevance, so standardized protocols for labelling NET components are required.
Tue Sep 10, 2019 21:14
In this study, we present a protocol for cortisol extraction from the fin and jawbone of sturgeon species. Fin and jawbone cortisol levels were further examined by comparing two washing solvents followed by ELISA assays. This study piloted the feasibility of jawbone cortisol as a novel stress indicator.
Tue Sep 10, 2019 20:13
Journal Name: Clinical Chemistry and Laboratory Medicine (CCLM)Issue: Ahead of print
Tue Sep 10, 2019 21:10
Journal Name: Clinical Chemistry and Laboratory Medicine (CCLM)Issue: Ahead of print
Tue Sep 10, 2019 17:03
Journal Name: Clinical Chemistry and Laboratory Medicine (CCLM)Issue: Ahead of print
Tue Sep 10, 2019 17:03
Journal Name: Clinical Chemistry and Laboratory Medicine (CCLM)Issue: Ahead of print
Tue Sep 10, 2019 17:03
Journal Name: Clinical Chemistry and Laboratory Medicine (CCLM)Issue: Ahead of print
Tue Sep 10, 2019 17:03
Journal Name: Clinical Chemistry and Laboratory Medicine (CCLM)Issue: Ahead of print
Tue Sep 10, 2019 17:03
Journal Name: Clinical Chemistry and Laboratory Medicine (CCLM)Issue: Ahead of print
Tue Sep 10, 2019 17:03
The trace amine-associated receptor 1 (TAAR1) agonist RO5263397 effect on sensory gating in C57BL/6 mice was studied. Sensory gating is a mechanism for dosing and filtering the incoming information, by which the brain regulates the responses to sensory stimuli coming from the environment. Sensory gating deficit is considered to be one of the schizophrenia endophenotypes. TAAR1 agonist at a 1 mg/kg dosage contributed to the sensory gating index (S1–S2) increase. Sensory gating index rose due to the...
Mon Sep 09, 2019 03:00
Objective We previously showed differences in brain grey matter volume changes between patients with early-onset adult depression (EOD) and late-onset adult depression (LOD). Here, we aim to identify whether cortical thickness (CT) is affected by the age of onset in patients with depression. Methods High-resolution MRI images were obtained for 54 major depressive disorder (MDD) patients with EOD, 58 patients with LOD, 57 young healthy controls (HCs), and 58 aged HCs. Depression severity was...
Mon Sep 09, 2019 03:00
Dendritic spines are divided into four subtypes, namely, Mushroom, Stubby, Thin, and Branched. The mushroom-shaped spines are related to learning and memory. Previous studies have shown that the dendritic cell factor 1 (Dcf1, a transmembrane protein) affects the memory process and regulates the development of dendritic spines by inhibiting the expression of lipocalin 2 (Lcn2, a member of the family containing over 20 small secreted proteins). However, the exact subtype of dendritic spines that are...
Mon Sep 09, 2019 03:00
AlkB family proteins are enzymes that repair alkylated DNA and RNA by oxidative demethylation. Nine homologs have been identified and characterized in mammals. ALKBH1 is conserved among metazoans including Drosophila. Although the ALKBH1 mouse homolog, Alkbh1 functions in neurogenesis, it currently remains unclear whether ALKBH1 plays a role in neuronal disorders induced by ultraviolet-induced DNA damage. We herein demonstrated that the Drosophila ALKBH1 homolog, AlkB contributed to recovery from...
Mon Sep 09, 2019 03:00
Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels are glycoproteins N-glycosylated at a specific asparagine residue in the S5-S6 linker region. Previous reports suggested that N-glycosylation-deficient HCN2 N380Q (NQ) channels fail to properly target to the plasma membrane and are unable to form functional ion channels. HCN channels are known to homo- and hetero-oligomerize and it is not known whether HCN2-NQ subunits can oligomerize with wild type (wt) N-glycosylated subunits to...
Mon Sep 09, 2019 03:00
Objective Growing evidences suggest systemic pathogen-induced neuroimmune interaction is a major risk factor for several neurological disorders. Our goal was to investigate whether asymptomatic peripheral carriage of Staphylococcus aureus, a widespread opportunistic pathogen, could modulate selective molecular features in brain tissues. Methods To address this, a peripheral infection model was developed by challenging Wistar rats repeatedly with a clinical strain of S. aureus. Animals infected...
Mon Sep 09, 2019 03:00
Objectives: The aim of this study was to determine the expression level of immunoproteasome and its clinical significance in glioma preliminarily. Furthermore, we studied the function and molecular mechanism of proteasome inhibitor ONX 0912 on glioma cell. Materials and methods: The expression of immunoproteasome in glioma and tumor-adjacent brain tissues was detected by western blot. Immunohistochemical technique was used to detect the expression of low-molecular-mass polypeptide 7 in 55...
Mon Sep 09, 2019 03:00
The modulation of cerebral activity could induce plastic changes in the cerebral cortex and contribute to motor rehabilitation. Focal vibration over lower-extremity muscles has therapeutic effects on the impaired motor function for stroke patients, but the modulatory effects of focal vibration on brain activity are less known. To explore this problem, this experiment was designed and conducted, in which focal vibration (75 Hz) was applied over the right Achilles tendon of 14 healthy subjects and...
Mon Sep 09, 2019 03:00
Objective: Propofol is broadly utilized for maintaining anesthesia. Propofol affects neurodegeneration and neurogenesis by regulation of autophagy via effects on intracellular calcium homeostasis. The underlying molecular mechanism, however, is still unclear. Methods: In the present research, we systematically analyzed the effect of propofol on mouse neuronal cells (cell line: HT-22). Cell Counting Kit-8 assays were utilized to examine cell proliferation. Flow cytometry was used to determine...
Mon Sep 09, 2019 03:00
3,4-Dihydroxyphenylethanol (DOPET) is a potent antioxidant polyphenolic compound. In this study, our objective was to investigate the underlying mechanism of the neuroprotective role of DOPET in attenuating spinal cord injury (SCI). Initially, SCI was induced by performing surgical laminectomy on the rats at T10-T12 level. Then, the neurological function-dependent locomotion was measured using Basso Beattie Bresnahan score, which declined in the SCI-induced group. Increased antioxidant levels such...
Mon Sep 09, 2019 03:00
Background. Pneumocystis pneumonia (PCP) remains a common opportunistic infection in immunosuppressed individuals. Current studies showed that multiple immune cells and cytokines took part in the host defense against Pneumocystis (PC). However, the roles of IL-17 and IL-10 in the development of PCP have not been elucidated. Methods. IL-10 and IL-17 levels in serum from PCP mice were detected via ELISA. The percentages of B10 cells, IL-10+ macrophages, and IL-10+ T cells in the lung from IL-17 PCP...
Tue Sep 10, 2019 19:05
Pregnancy-associated plasma protein A (PAPP-A) was previously reported to be an inflammatory biomarker and a prognostic marker of acute coronary syndrome (ACS) and involved in the process of atherosclerosis and plaque rupture. However, the role of PAPP-A in inflammation is poorly understood. In this study, we aimed to investigate the role of PAPP-A in macrophage activation and inflammatory cytokine production. RAW264.7 macrophages were treated with or without PAPP-A. Reverse-transcriptase quantitative...
Tue Sep 10, 2019 18:05
At the heart of the revolutionary otoscope from U.S. company OtoNexus Medical Technologies is a unique CMUT chip from Fraunhofer IPMS based on ultrasound technology. Diagnosing infections of the middle ear is prone to a great deal of subjectivity. Antibiotics being the typical treatment option, way too many patients are treated who are actually not infected. This can result in the development of antibiotic-resistant bacteria and a host of other problems. Researchers at the Fraunhofer...
Tue Sep 10, 2019 20:29
At Purdue University, engineers have created a practical way of giving tubes, microchannels, and other hollow components superhydrophobic properties. The technique will improve existing medical devices and make new ones, particularly originating from the field of microfluidics, possible. Currently, there are a number of techniques to make a surface repel water so much that a water drop placed on it retains its spherical shape. These are impractical, for one reason or another, when...
Tue Sep 10, 2019 18:49
While there has been a good deal of progress in designing ever more advanced visual prostheses, some of the more impressive existing devices try to take over the functionality of the eye by directly stimulating the optic nerve or even the visual cortex of the brain. While this is impressive in itself, researchers at SUNY Downstate Health Sciences University in Brooklyn, New York, are now embarking on the development of a new visual prosthetic system that involves genetically engineering neural cells,...
Tue Sep 10, 2019 18:34
The scalable synthesis of carboxy‐MIDA‐boronate, one of the simplest boron‐containing building blocks, is described. Carboxy‐MIDA‐boronate can undergo a diverse range of transformations, providing access to an extensive scope of borylated products. Carboxy‐MIDA‐boronate is found to possess unusual reactivity towards nucleophiles. Abstract The synthesis and applications of carboxy‐MIDA‐boronate, a novel C1 building block, are described. This molecule is accessible via a ruthenium tetraoxide‐mediated...
Tue Sep 10, 2019 20:15
“Chemistry is fun because it is strongly unpredictable and, despite what we may claim in our proposals, chance discoveries are absolutely the most enjoyable. My favorite chemical effects are kinetic isotope effects. Huge (and not so huge) differences and subsequent understanding from sometimes so little changes …” Find out more about Jérôme Lacour in his Author Profile.
Tue Sep 10, 2019 20:15
A new strategy for enantioselective synthesis of axially chiral naphthyl‐indoles was established by catalytic asymmetric addition reactions of racemic naphthyl‐indoles with bulky electrophiles. Under chiral Brønsted acid (B*−H) catalysis, azodicarboxylates and o‐hydroxybenzyl alcohols served as bulky but reactive electrophiles that were attacked by C2‐unsubstituted naphthyl‐indoles, affording axially chiral naphthyl‐indoles in good yields and enantioselectivities. Abstract A new strategy for...
Tue Sep 10, 2019 20:15
One way or another: Two divergent routes of a weakly anti‐aromatic and Lewis‐acidic pentaarylborole with the monolvalent Group 13 cyclopentadienyl compounds AlCp* and GaCp* have been identified. The energetic accessibility of their two lone pairs of electrons determines whether a redox reaction to form a neutral heteroleptic borolediide/cyclopentadienyl “aluminocene” or formation of a Lewis‐base adduct with a dative Ga−B bond is observed. Abstract The pentaaryl borole (Ph*C)4BXylF [Ph*=3,5‐tBu2(C6H3);...
Tue Sep 10, 2019 20:15
“My greatest achievement has been successfully introducing students to research and seeing the lightbulb come on in their heads. The most exciting thing about my research is pursuing the unknown …” Find out more about Thomas P. Russell in his Author Profile.
Tue Sep 10, 2019 20:15
Bimetallic catalysis: Using atomic layer modification of CuO by ZnO, in situ reduced CuZn nanowires are prepared as selective catalysts for CO2 electroreduction to ethanol. The excessive CO formation catalyzed by Zn and the fast kinetics of *CH3 formation at high overpotential on Cu are believed to be critical in determining the selectivity of ethanol. Abstract Electrochemical reduction of carbon dioxide, if powered by renewable electricity, could serve as a sustainable technology for carbon...
Tue Sep 10, 2019 20:15
Changing hands: The stimuli‐induced change of macrocycle position in a rotaxane is exploited to switch the effective handedness of a catalytic center on the axle. The dynamic switching is used to reverse the enantioselectivity of an enamine‐mediated conjugate addition. Abstract We report on a switchable rotaxane molecular shuttle that features a pseudo‐meso 2,5‐disubstituted pyrrolidine catalytic unit on the axle whose local symmetry is broken according to the position of a threaded benzylic...
Tue Sep 10, 2019 20:15
More charge, more clarity: Like the cyclobutadiene dianion, the cyclic, polyphosphorus cyclo‐P4 dianion creates an electronic structure ambiguity when found in neutral transition‐metal complexes. Dianionic cyclo‐P4 complexes are presented that can be viewed as the simple binding of [cyclo‐P4]2− to a metal. Abstract Relative to other cyclic poly‐phosphorus species (that is, cyclo‐Pn ), the planar cyclo‐P4 group is unique in its requirement of two additional electrons to achieve aromaticity....
Tue Sep 10, 2019 20:15
The study of palladium(IV) species has great implications for Pd(II)/Pd(IV)‐mediated catalysis. However, most of the Pd(IV) complexes rapidly decompose under ambient conditions, which makes the isolation, characterization and further reactivity study very challenging. The reported ancillary ligand platforms to stabilize Pd(IV) species are dominated by chelating N‐donors such as bipyridines. In this work, we present a Pd(IV) system with scarcely used C‐donors as the supporting platform. The anionic...
Tue Sep 10, 2019 13:50
We demonstrate a simple and effective chemical equilibrium regulation strategy to improve the efficiency of electrochemical ammonia synthesis by constructing a low‐pressure electrochemical reaction system. The strategy of transferring the nitrogen reduction reaction from ambient conditions to a low‐pressure environment can not only accelerate the activation of the N≡N triple bond but also effectively inhibit the competition reaction of hydrogen evolution while promoting the dissolution and diffusion...
Tue Sep 10, 2019 13:49
One of the great challenges in chemistry is the development of innovative catalytic processes that save our resources by carrying out various useful chemical transformations with low energy and cost input. In this context, significant opportunities currently open up for technologies based on electrocatalysis and photo(electro)catalysis, as these can drive various technologically relevant catalytic redox transformations under very mild conditions. However, many catalytic processes allow for formation...
Tue Sep 10, 2019 13:48
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