A comprehensive understanding of protein function demands correlating structure and dynamic changes. Using time-resolved serial synchrotron crystallography, we visualized half-of-the-sites reactivity and correlated molecular-breathing motions in the enzyme fluoroacetate dehalogenase. Eighteen time points from 30 milliseconds to 30 seconds cover four turnover cycles of the irreversible reaction. They reveal sequential substrate binding, covalent-intermediate formation, setup of a hydrolytic water...
Thu Sep 12, 2019 20:40
In eukaryotic membranes, type IV P-type adenosine triphosphatases (P4-ATPases) mediate the translocation of phospholipids from the outer to the inner leaflet and maintain lipid asymmetry, which is critical for membrane trafficking and signaling pathways. Here, we report the cryo–electron microscopy structures of six distinct intermediates of the human ATP8A1-CDC50a heterocomplex at resolutions of 2.6 to 3.3 angstroms, elucidating the lipid translocation cycle of this P4-ATPase. ATP-dependent phosphorylation...
Thu Sep 12, 2019 20:40
Kelso et al. (Reports, 12 April 2019, p. 166) claim that inorganic epitaxial films were deposited onto single-crystal or single-crystal–like substrates by spin coating. The epitaxial relationships were determined by x-ray diffraction. According to their pole figures, we estimate that each of their films contains only 4.1% to 25.5% epitaxial grains. None of their films can be considered epitaxial.
Thu Sep 12, 2019 20:40
Tetrahexahedral particles (~10 to ~500 nanometers) composed of platinum (Pt), palladium, rhodium, nickel, and cobalt, as well as a library of bimetallic compositions, were synthesized on silicon wafers and on catalytic supports by a ligand-free, solid-state reaction that used trace elements [antimony (Sb), bismuth (Bi), lead, or tellurium] to stabilize high-index facets. Both simulation and experiment confirmed that this method stabilized the {210} planes. A study of the PtSb system showed that the...
Thu Sep 12, 2019 20:40
Terminal copper-nitrenoid complexes have inspired interest in their fundamental bonding structures as well as their putative intermediacy in catalytic nitrene-transfer reactions. Here, we report that aryl azides react with a copper(I) dinitrogen complex bearing a sterically encumbered dipyrrin ligand to produce terminal copper nitrene complexes with near-linear, short copper–nitrenoid bonds [1.745(2) to 1.759(2) angstroms]. X-ray absorption spectroscopy and quantum chemistry calculations reveal a...
Thu Sep 12, 2019 20:40
Neonicotinoids are neurotoxic insecticides widely used as seed treatments, but little is known of their effects on migrating birds that forage in agricultural areas. We tracked the migratory movements of imidacloprid-exposed songbirds at a landscape scale using a combination of experimental dosing and automated radio telemetry. Ingestion of field-realistic quantities of imidacloprid (1.2 or 3.9 milligrams per kilogram body mass) by white-crowned sparrows (Zonotrichia leucophrys) during migratory...
Thu Sep 12, 2019 20:40
Abstract Background and aims In routine clinical practice, early discontinuation of newly initiated glucose-lowering medications (GLM) is relatively common. We herein evaluated if the clinical characteristics associated with early discontinuation of dapagliflozin were different from those associated with early discontinuation of other GLM. Methods The DARWIN-T2D...
Thu Sep 12, 2019 03:00
Chronic obstructive pulmonary disease (COPD) is characterized by persistent respiratory symptoms and airflow limitation. Patients may have episodes of increased respiratory symptoms (exacerbations) that are moderate (leading to enhanced treatment) or severe (leading to hospitalization).…
Thu Sep 12, 2019 03:00
In China, 120 million persons have chronic kidney disease, a prevalence that is projected to increase. Anemia is present in more than 90% of the 500,000 patients who undergo dialysis and is a complication that contributes to increased morbidity and mortality. Treatment for anemia is recommended by…
Thu Sep 12, 2019 03:00
Preterm delivery (defined as delivery before 37 weeks of gestation) occurs in 15 million pregnancies each year and is the leading cause of early childhood complications and death; early preterm deliveries (defined as delivery before 34 weeks of gestation) represent approximately 20% of all preterm…
Thu Sep 12, 2019 03:00
Chronic kidney disease is a global public health challenge that affects approximately 10% of the population worldwide, including 120 million people in China. Anemia (defined as a hemoglobin level of In China, this situation is reflected by low hemoglobin levels in patients who initiate dialysis,…
Thu Sep 12, 2019 03:00
Publication date: Available online 11 September 2019Source: Revue des Maladies RespiratoiresAuthor(s): P. Créquit, J. Cadranel, H. Mal, B. Grigoriu, T. Berghmans
Thu Sep 12, 2019 21:34
Publication date: Available online 11 September 2019Source: Revue des Maladies RespiratoiresAuthor(s): J. Taytard, P. Boizeau, C. Alberti, N. BeydonRésuméIntroductionLa capacité de transfert du monoxyde de carbone (DLCO) est mesurée par la méthode de référence durant une apnée (DLCOap) ou, en cas d’impossibilité d’obtenir une DCLOap, en respiration spontanée avec d’autres méthodes dont celle de la réinspiration (rebreathing ; rb). L’objectif était d’évaluer la faisabilité en clinique de la mesure...
Thu Sep 12, 2019 21:3
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