In vitro safety evaluation of a povidone-iodine solution applied to human nasal epithelial cells............. Application of Nasodine to HNEC-ALI cultures in vitro for up to 30 minutes was not toxic and did not affect the paracellular permeability or CBF.

In vitro safety evaluation of a povidone-iodine solution applied to human nasal epithelial cells.:

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In vitro safety evaluation of a povidone-iodine solution applied to human nasal epithelial cells.

Int Forum Allergy Rhinol. 2020 Apr 06;:

Authors: Ramezanpour M, Smith JLP, Psaltis AJ, Wormald PJ, Vreugde S

Abstract

BACKGROUND: Nasal topical treatments can provide an effective method of disease control for patients suffering from chronic rhinosinusitis (CRS). However, some frequently used formulations lack adequate evaluation on their safety. This study investigated the effect of 0.5% Povidone-Iodine (Nasodine) on the sinonasal epithelial barrier and ciliated human nasal epithelial cells in vitro.

METHODOLOGY/PRINCIPAL: Nasodine was applied to air-liquid interface (ALI) cultures of primary human nasal epithelial cells (HNEC) from CRS patients. Epithelial barrier structure was assessed by measuring the Trans Epithelial Electrical Resistance (TEER), paracellular permeability and immunolocalisation of the Zona Occludens-1 (ZO-1) tight junction protein. Toxicity and Cilia Beat Frequency (CBF) were also studied.

RESULTS: Nasodine was not toxic and did not have detrimental effects on the paracellular permeability or CBF. Nasodine did not show a significant reduction in TEER with a 5 minute exposure, however, with a 30 minute exposure there was a significant reduction in TEER 60 minutes and 4 hours after exposure.

CONCLUSION: Application of Nasodine to HNEC-ALI cultures in vitro for up to 30 minutes was not toxic and did not affect the paracellular permeability or CBF. This article is protected by copyright. All rights reserved.

PMID: 32250552 [PubMed - as supplied by publisher]