Methods and Protocols,

MPs, Vol. 3, Pages 38: Preconditioning of Bioactive Glasses before Introduction to Static Cell Culture: What Is Really Necessary?

MPs, Vol. 3, Pages 38: Preconditioning of Bioactive Glasses before Introduction to Static Cell Culture: What Is Really Necessary? Methods and Protocols doi: 10.3390/mps3020038 Authors: Hohenbild Arango-Ospina Moghaddam Boccaccini Westhauser Due to their high bioreactivity, the in-vitro analysis of bioactive glasses (BGs) can be challenging when it comes to maintaining a physiological pH. To improve BG biocompatibility, a heterogenic spectrum of preconditioning approaches,...

Methods and Protocols

Sat May 09, 2020 03:00

MPs, Vol. 3, Pages 37: Positive Selection of Specific Antibodies Produced against Fusion Proteins

MPs, Vol. 3, Pages 37: Positive Selection of Specific Antibodies Produced against Fusion Proteins Methods and Protocols doi: 10.3390/mps3020037 Authors: Lukas Kramberger-Kaplan Tina Austerlitz Holger Bohlmann A method for the positive selection of specific antibodies for target proteins expressed as fusion proteins for the production of antiserum is presented. As proof of concept, the fusion protein FLAG::His::GFP::His::FLAG was expressed in Escherichia coli, purified, and used...

Methods and Protocols

Fri May 08, 2020 03:00

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