Related ArticlesEnteral and Parenteral Order Writing Survey-A Collaborative Evaluation Between the Academy of Nutrition and Dietetics' Dietitians in Nutrition Support Dietetics Practice Group and the American Society for Parenteral and Enteral Nutrition (ASPEN) Dietetics Practice Section. J Acad Nutr Diet. 2020 Mar 24;: Authors: Peterson S, Dobak S, Phillips W, Malone A, Ireton-Jones C, Haney A, Rybicki M, Guenter P Abstract INTRODUCTION: In 2014 and...
Wed Apr 01, 2020 14:32
Related ArticlesMorphological characteristics of the lateral ankle ligament complex. Surg Radiol Anat. 2020 Mar 30;: Authors: Kobayashi T, Suzuki D, Kondo Y, Tokita R, Katayose M, Matsumura H, Fujimiya M Abstract PURPOSE: The relevance of each ligament comprising the lateral ankle ligament complex, including the anterior talofibular ligament (ATFL), calcaneofibular ligament (CFL), and posterior talofibular ligament (PTFL), has not been sufficiently...
Wed Apr 01, 2020 14:31
Related ArticlesMeniscofibular ligament: how much do we know about this structure of the posterolateral corner of the knee: anatomical study and review of literature. Surg Radiol Anat. 2020 Mar 29;: Authors: Natsis K, Karasavvidis T, Kola D, Papadopoulos S, Totlis T Abstract PURPOSE: The present study is a systematic review of a relatively unknown structure of the posterolateral corner of the knee, the meniscofibular ligament (MFL), aiming at summarizing...
Wed Apr 01, 2020 14:31
Related ArticlesMurine Precision-Cut Liver Slices as an Ex Vivo Model of Liver Biology. J Vis Exp. 2020 Mar 14;(157): Authors: Pearen MA, Lim HK, Gratte FD, Fernandez-Rojo MA, Nawaratna SK, Gobert GN, Olynyk JK, Tirnitz-Parker JEE, Ramm GA Abstract Understanding the mechanisms of liver injury, hepatic fibrosis, and cirrhosis that underlie chronic liver diseases (i.e., viral hepatitis, non-alcoholic fatty liver disease, metabolic liver disease, and...
Wed Apr 01, 2020 14:31
Related ArticlesMorphological and Functional Evaluation of Axons and their Synapses during Axon Death in Drosophila melanogaster. J Vis Exp. 2020 Mar 16;(157): Authors: Paglione M, Rosell AL, Chatton JY, Neukomm LJ Abstract Axon degeneration is a shared feature in neurodegenerative disease and when nervous systems are challenged by mechanical or chemical forces. However, our understanding of the molecular mechanisms underlying axon degeneration remains...
Wed Apr 01, 2020 14:31
Related ArticlesHigh-Frequency Ultrasound Echocardiography to Assess Zebrafish Cardiac Function. J Vis Exp. 2020 Mar 12;(157): Authors: Evangelisti A, Schimmel K, Joshi S, Shah K, Fisch S, Alexander KM, Liao R, Morgado I Abstract The zebrafish (Danio rerio) has become a very popular model organism in cardiovascular research, including human cardiac diseases, largely due to its embryonic transparency, genetic tractability, and amenity to rapid, high-throughput...
Wed Apr 01, 2020 14:31
Related ArticlesValidating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example. J Vis Exp. 2020 Mar 11;(157): Authors: Oude Munnink BB, Nieuwenhuijse DF, Sikkema RS, Koopmans M Abstract Whole genome sequencing can be used to characterize and to trace viral outbreaks. Nanopore-based whole genome sequencing protocols have been described for several different viruses. These approaches utilize an overlapping amplicon-based approach which...
Wed Apr 01, 2020 14:31
Related ArticlesVisualization of Estrogen Receptors in Colons of Mice with TNBS-Induced Crohn's Disease using Immunofluorescence. J Vis Exp. 2020 Mar 12;(157): Authors: Jacenik D, Zielińska M, Michlewska S, Fichna J, Krajewska WM Abstract Crohn's disease is the most diagnosed type of inflammatory bowel disease. Chronic inflammation developing in the intestine leads to peristalsis disorder and damage of intestinal mucosa and seems to be associated...
Wed Apr 01, 2020 14:31
Related ArticlesCulture Methods to Determine the Limit of Detection and Survival in Transport Media of Campylobacter Jejuni in Human Fecal Specimens. J Vis Exp. 2020 Mar 10;(157): Authors: Buss JE, Thacker E, Santiago M Abstract A culture from human stool for diagnosis of Campylobacter-based intestinal illness takes several days, a wait that taxes the fortitude of the physician and the patient. A culture is also prone to false negative results from...
Wed Apr 01, 2020 14:31
Related ArticlesCharacterizing Cellular Proteins with In-cell Fast Photochemical Oxidation of Proteins. J Vis Exp. 2020 Mar 11;(157): Authors: Chea EE, Rinas A, Espino JA, Jones LM Abstract Fast photochemical oxidation of proteins (FPOP) is a hydroxyl radical protein footprinting method used to characterize protein structure and interactions. FPOP uses a 248 nm excimer laser to photolyze hydrogen peroxide producing hydroxyl radicals. These radicals...
Wed Apr 01, 2020 14:31
Related ArticlesUntargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain. J Vis Exp. 2020 Mar 13;(157): Authors: Abbiss H, Gummer JPA, Francki M, Trengove RD Abstract Understanding the interactions between genes, the environment and management in agricultural practice could allow more accurate prediction and management of product yield and quality. Metabolomics data provides a read-out of these interactions at...
Wed Apr 01, 2020 14:31
Related ArticlesIn Vitro Model of Coronary Angiogenesis. J Vis Exp. 2020 Mar 10;(157): Authors: Large CL, Vitali HE, Whatley JD, Red-Horse K, Sharma B Abstract Here, we describe an in vitro culture assay to study coronary angiogenesis. Coronary vessels feed the heart muscle and are of clinical importance. Defects in these vessels represent severe health risks such as in atherosclerosis, which can lead to myocardial infarctions and heart failures...
Wed Apr 01, 2020 14:31
Related ArticlesGeneration of Mosaic Mammary Organoids by Differential Trypsinization. J Vis Exp. 2020 Mar 11;(157): Authors: Rubio S, Cazares O, Macias H, Hinck L Abstract Organoids offer self-organizing, three-dimensional tissue structures that recapitulate physiological processes in the convenience of a dish. The murine mammary gland is composed of two distinct epithelial cell compartments, serving different functions: the outer, contractile myoepithelial...
Wed Apr 01, 2020 14:31
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Wed Apr 01, 2020 14:31
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Wed Apr 01, 2020 14:31
Related ArticlesThermocapillary Convection Space Experiment on the SJ-10 Recoverable Satellite. J Vis Exp. 2020 Mar 11;(157): Authors: Duan L, Yin Y, Wang J, Kang Q, Wu D, Jiang H, Zhang P, Hu L Abstract Thermocapillary convection is an important research subject in microgravity fluid physics. The experimental study on surface waves of thermocapillary convection in an annular liquid pool is one of the 19 scientific experimental projects on the SJ-10...
Wed Apr 01, 2020 14:31
Related ArticlesTomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease. J Vis Exp. 2020 Mar 11;(157): Authors: Morcillo RJL, Zhao A, Tamayo-Navarrete MI, García-Garrido JM, Macho AP Abstract Ralstonia solanacearum is a devastating soil borne vascular pathogen that can infect a large range of plant species, causing an important threat to agriculture. However, the...
Wed Apr 01, 2020 14:31
Related ArticlesCovalent Attachment of Single Molecules for AFM-based Force Spectroscopy. J Vis Exp. 2020 Mar 16;(157): Authors: Kolberg A, Wenzel C, Hugel T, Gallei M, Balzer BN Abstract Atomic force microscopy (AFM)-based single molecule force spectroscopy is an ideal tool for investigating the interactions between a single polymer and surfaces. For a true single molecule experiment, covalent attachment of the probe molecule is essential because...
Wed Apr 01, 2020 14:31
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Wed Apr 01, 2020 14:31
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Wed Apr 01, 2020 14:31
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Wed Apr 01, 2020 14:31
Related ArticlesAnalysis of Congenital Heart Defects in Mouse Embryos Using Qualitative and Quantitative Histological Methods. J Vis Exp. 2020 Mar 10;(157): Authors: Ball K, Kinne R, Aguirre A Abstract Congenital heart defects (CHD) are the most common type of birth defect in humans, affecting up to 1% of all live births. However, the underlying causes for CHD are still poorly understood. The developing mouse constitutes a valuable model for the...
Wed Apr 01, 2020 14:31
Related ArticlesEvent Related Potentials (ERPs) and other EEG Based Methods for Extracting Biomarkers of Brain Dysfunction: Examples from Pediatric Attention Deficit/Hyperactivity Disorder (ADHD). J Vis Exp. 2020 Mar 12;(157): Authors: Ogrim G, Kropotov JD Abstract Neuropsychiatric diagnoses like ADHD are based on subjective methods like interviews, rating scales and observations. There is a need for more brain-based supplements. Stimulant medication...
Wed Apr 01, 2020 14:31
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Wed Apr 01, 2020 14:31
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Wed Apr 01, 2020 14:31
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