Publication date: Available online 26 August 2019Source: Acta BiomaterialiaAuthor(s): Xiaolin Lu, Ronghe Chen, Jing Lv, Weicai Xu, Hongjiang Chen, Zebin Ma, Shanshan Huang, Shi Li, Heng Liu, Jun Hu, Liming NieAbstractUnsatisfactory diagnosis and therapy of osteomyelitis are still common but challenging issues for clinicians. To overcome these problems, a bacterial inflammation-specific multifunctional agent, denoted bovine serum albumin-manganese dioxide-ubiquicidin29-41-indocyanine green (ICG) -gentamicin...
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Publication date: Available online 26 August 2019Source: Acta BiomaterialiaAuthor(s): Lisanne M.M. Gommers, Katarzyna Skrzypek, Lydia Bolhuis-Versteeg, Nicole E.T. Pinckaers, Rob Vrijhof, Jenny van der Wijst, Jeroen H.F. de Baaij, Dimitrios Stamatialis, Joost G.J. HoenderopAbstractIntestinal enterocytes are key players in the absorption of magnesium (Mg2+) and calcium (Ca2+). Understanding the exact molecular mechanisms by which their absorption behavior is regulated could greatly improve treatment...
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Publication date: Available online 26 August 2019Source: Acta BiomaterialiaAuthor(s): Selda Sherifova, Gerhard Sommer, Christian Viertler, Peter Regitnig, Thomas Caranasos, Margaret Anne Smith, Boyce E. Griffith, Ray W. Ogden, Gerhard A. HolzapfelAbstractCurrent clinical practice for aneurysmatic interventions is often based on the maximum diameter of the vessel and/or on the growth rate, although rupture can occur at any diameter and growth rate, leading to fatality. For 27 medial samples obtained...
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Publication date: Available online 26 August 2019Source: Acta BiomaterialiaAuthor(s): Akihiro Nishiguchi, Yukari Kurihara, Tetsushi TaguchiAbstractDespite the success of minimally-invasive endoscopic submucosal dissection (ESD) for the treatment of early gastrointestinal cancer, additional symptoms after ESD, including contracture, perforation, bleeding, and esophageal stricture remain. Conventional wound dressings were ineffective in preventing stricture because of poor stability of underwater-adhesives...
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Biodegradation kinetics of microcystins-LR crude extract by Lysinibacillus boronitolerans strain CQ5
Abstract Purpose As the most common variant of microcystins (MCs), microcystin-LR (MCLR) is a kind of toxins produced by some species of harmful cyanobacteria and more and more attention has been paid to it. Biodegradation has been extensively investigated and recognized to be a cost-efficient and environmentally benign method for MC clean-up. In order to further research the growth characteristics of strain and the biodegradation characteristics...
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Antibiotics, Vol. 8, Pages 131: Phage Therapy with a focus on the Human Microbiota Antibiotics doi: 10.3390/antibiotics8030131 Authors: Ganeshan Hosseinidoust Bacteriophages are viruses that infect bacteria. After their discovery in the early 1900s, bacteriophages were a primary cure against infectious disease for almost 25 years, before being completely overshadowed by antibiotics. With the rise of antibiotic resistance, bacteriophages are being explored again for their antibacterial...
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Antibiotics, Vol. 8, Pages 130: Comparative In Vitro Activities of First and Second-Generation Ceragenins Alone and in Combination with Antibiotics Against Multidrug-Resistant Klebsiella pneumoniae Strains Antibiotics doi: 10.3390/antibiotics8030130 Authors: Berna Ozbek-Celik Damla Damar-Celik Emel Mataraci-Kara Cagla Bozkurt-Guzel Paul B. Savage Objectives: The ceragenins, or CSAs, were designed to mimic the activities of antimicrobial peptides and represent a new class...
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Related ArticlesGPR39 agonist TC-G 1008 promotes osteoblast differentiation and mineralization in MC3T3-E1 cells. Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):3569-3576 Authors: Chai X, Zhang W, Chang B, Feng X, Song J, Li L, Yu C, Zhao J, Si H Abstract Osteoporosis-related bone fracture and falls have a severe impact on patients' daily lives. Osteoblasts are bone-building cells that play a vital role in bone formation and remodeling. Imbalanced osteoblast...
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Related ArticlesThe study of mechanism of miR-34c-5p targeting FLOT2 to regulate proliferation, migration and invasion of osteosarcoma cells. Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):3559-3568 Authors: Wang Y, Wang X, Tang J, Su X, Miao Y Abstract Objective: Osteosarcoma is one of the most common malignancies in children and adolescents. Studies have shown that miR-34c-5p is involved in the progression of various cancers. To explore the effects...
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How the Membrane Attack Complex Damages the Bacterial Cell Envelope and Kills Gram‐Negative Bacteria
Recent work has revealed that local assembly of membrane attack complex (MAC) pores at the surface by convertase enzymes is essential to damage the bacterial cell envelope and kill Gram‐negative bacteria. How these insights have changed the current view on MAC assembly and how MAC pores kill bacteria are discussed here. The human immune system can directly lyse invading micro‐organisms and aberrant host cells by generating pores in the cell envelope, called membrane attack complexes (MACs). Recent...
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Metazoan cells possess two genomes (i.e., nuclear and mitochondrial), but the nuclear DNA was thought to exclusively encode regulators of both genomes. Recently, Bérénice A. Benayoun and Changhan Lee found that a small peptide encoded in the mitochondrial genome, MOTS‐c, directly regulates the nuclear genome, revealing an integrated bi‐genomic basis of gene regulation. More details can be found in article number 1900046 by Bérénice A. Benayoun and Changhan Lee. DOI: 10.1002/bies.201900046.
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Abstract The somatic integration definition of life is familiar from the debate on the determination of death, with some bioethicists arguing that it supports brain death while others argue that some brain‐dead bodies exhibit sufficient somatic integration for biological life. I argue that on either interpretation, the somatic integration definition of life implies that neither the preimplantation embryo nor the postimplantation embryo meet the somatic integration threshold condition for organismal...
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Publication date: Available online 26 August 2019Source: Journal of BiomechanicsAuthor(s): Gianni PedrizzettiAbstractThe hemodynamic forces exchanged between the blood flowing in the heart and the myocardium are recently receiving attention as an important marker of cardiac function. The increasing interest was associated to the advent of advanced imaging methods able to measuring the blood velocity field inside the cardiac chambers, from which flow forces are obtained as volume integral of the fluid...
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Biomedicines, Vol. 7, Pages 64: A Proposed Framework for Patient-Focused Policy at the U.S. Food and Drug Administration Biomedicines doi: 10.3390/biomedicines7030064 Authors: Carrie M. Kuehn Medical product sponsors are encouraged to include the patient perspective in their medical product development strategy to inform product design, augment regulatory submissions, argue for alternative clinical trial designs, or to support indications in specific patient populations. The goal is...
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Biomolecules, Vol. 9, Pages 418: Orientin Induces G0/G1 Cell Cycle Arrest and Mitochondria Mediated Intrinsic Apoptosis in Human Colorectal Carcinoma HT29 Cells Biomolecules doi: 10.3390/biom9090418 Authors: Thangaraj Balasubramanian Park Natesan Liu Manju Colorectal carcinoma is one of the utmost diagnosed cancer with a steep increase in mortality rate. The incidence has been increasing in developing countries like India due to a westernization life style. Flavonoids...
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Biomolecules, Vol. 9, Pages 417: System Analysis of MIRNAs in Maize Internode Elongation Biomolecules doi: 10.3390/biom9090417 Authors: Chuanxi Peng Xing Wang Tianyu Feng Rui He Mingcai Zhang Zhaohu Li Yuyi Zhou Liusheng Duan MicroRNAs (miRNAs), the post-transcriptional gene regulators, are known to play an important role in plant development. The identification of differentially expressed miRNAs could better help us understand the post-transcriptional regulation...
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Revisiting the reduction of stochastic models of genetic feedback loops with fast promoter switching
Propensity functions of the Hill-type are commonly used to model transcriptional regulation in stochastic models of gene expression. This leads to an effective reduced master equation for the mRNA and protein dynamics only. Based on deterministic considerations, it is often stated or tacitly assumed that such models are valid in the limit of rapid promoter switching. Here, starting from the chemical master equation describing promoter-protein interactions, mRNA transcription, protein translation...
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Despite the key role of beat-to-beat action potential (AP) variability in the onset of ventricular arrhythmias at high pacing rate, the knowledge of the involved dynamics and of effective prognostic parameters is largely incomplete. Electrical restitution (ER), the way AP duration (APD) senses changes in preceding cycle length (CL), has been used to monitor transition to arrhythmias. The use of standard ER (sER) though, is controversial, not always suitable for in-vivo, and only rarely for clinical...
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Amino acid transport into the cell is often coupled to the proton electrochemical gradient, as found in the solute carrier (SLC) 36 family of proton coupled amino acid transporters (PATs). Although no structure of a human PAT exists, the crystal structure of a related homolog from bacteria, GkApcT, has recently been solved in an inward-occluded state and allows an opportunity to examine how protons are coupled to amino acid transport. Our working hypothesis is that release of the amino acid substrate...
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Publication date: 15 October 2019Source: Biosensors and Bioelectronics, Volume 143Author(s): Minghui Zhu, Xia Zhong, Hanmei Deng, Liaojing Huang, Ruo Yuan, Yali YuanAbstractHerein, by utilizing bipedal DNA walker as booster to adjust the distance of quencher ferrocene (Fc) and sensitizer methylene blue (MB) to photoactive material perylene-3,4,9,10-tetracarboxylic acid (PTCA), a novel “on-off-super on” photoelectrochemical (PEC) biosensor was proposed for ultrasensitive detection of thrombin (TB)....
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Publication date: 15 October 2019Source: Biosensors and Bioelectronics, Volume 143Author(s): Weijing Yi, Ruili Cai, Dongfang Xiang, Yanxia Wang, Mengsi Zhang, Qinghua Ma, Youhong Cui, Xiuwu BianAbstractAn ultrasensitive photoelectrochemical (PEC) bioassay for determination of microRNA was proposed based on an integrative photoactive heterojunction nanomaterial to provide the basis of excellent PEC responses and an efficient redox cycling amplification system to improve the detection performances....
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Publication date: 15 October 2019Source: Biosensors and Bioelectronics, Volume 143Author(s): Kingkan Pungjunun, Sudkate Chaiyo, Narong Praphairaksit, Weena Siangproh, Astrid Ortner, Kurt Kalcher, Orawon Chailapakul, Eda MehmetiAbstractA disposable gas-sensing paper-based device (gPAD) was fabricated in origami design which integrates the gas adsorbent and the electrochemical detection zone in a single device. The gPAD for the determination of NOx gas uses a screen-printed graphene electrode modified...
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Publication date: 15 October 2019Source: Biosensors and Bioelectronics, Volume 143Author(s): Jianmei Yang, Xiaotong Huang, Chunfang Gan, Ruo Yuan, Yun XiangAbstractDespite the fact that the identification and detection of circulating tumor cells (CTCs) plays a critical role in cancer monitoring and diagnosis, it remains a major challenge to isolate and detect these cells, due to their extreme scarcity in peripheral blood. In this work, by coupling a dual recognition strategy and the commercial personal...
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Publication date: 15 October 2019Source: Biosensors and Bioelectronics, Volume 143Author(s): Dagan Zhang, Feika Bian, Lijun Cai, Tianfu Wang, Tiantian Kong, Yuanjin ZhaoAbstractMultiplexed detection of microRNA (miRNA) is of great value in clinical diagnosis. Here, a new type of polydopamine (PDA) encapsulated photonic crystal (PhC) barcodes are employed for target-triggering cycle amplification and hybridization chain reaction (HCR) to achieve multiplex miRNA quantification. The PDA-decorated PhC...
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Transferrin receptor (TfR) targeted binding peptide analog BP9a (CAHLHNRS) was connected with doxorubicin (DOX) via the thioether bond linkage to afford a BP9a‐DOX conjugate. It showed specific cellular uptake properly bY TfR overexpressed HepG2 human hepatoma cells, and exhibited selective cytotoxic effect on HepG2 cells. Abstract In this study, a peptide‐drug conjugate was designed and synthesized by connecting a transferrin receptor (TfR) targeted binding peptide analog BP9a (CAHLHNRS) with...
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The role of unstructured peptide terminus at the interface and the effect of β‐augmentation in association with PCSK9. The design with phosphorylated peptides mimics PCSK9‐LDLR interaction. Abstract PCSK9, a member of the proprotein convertase family, is a key negative regulator of hepatic low‐density lipoprotein receptor (LDLR) concentrations in the blood plasma and is associated with the risk of coronary artery disease (CAD). Peptide inhibitors designed to block PCSK9‐LDLR interactions could...
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The article describes evaluation of GSH‐Ag2S quantum dots (QDs) with strong emission in the near infrared region (NIR) as a substrate for GST enzyme via fluorometric and spectrophotometric methods. Luminescence of QDs quenched with CDNB but recovered by GST enzyme Changes in luminescence intensity found to be dependent of the concentrations of CDNB and the GST enzyme. Moreover, GSH‐Ag2S QDs acted a susctrate analogue of GST‐ pi enzyme in vitro Abstract Quantum dots (QD) are being evaluated as inorganic...
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AbstractIn nature anaerobic ammonium oxidation (anammox) and denitrification processes convert fixed nitrogen to gaseous nitrogen compounds, which are then released to the atmosphere. While anammox bacteria produce N2 from ammonium and nitrite, in the denitrification process nitrate and nitrite are converted to N2 and the greenhouse gas nitrous oxide (N2O). Furthermore, nitrite needed by the anammox bacteria can be supplied by nitrate reduction to nitrite. Consequently, the interplay between nitrogen-transforming...
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AbstractPolar lipid-derived fatty acids (PLFAs) and their stable carbon isotopes are frequently combined to characterize microbial populations involved in the degradation of organic matter, offering a link to biogeochemical processes and carbon sources used. However, PLFA patterns derive from multiple species and may be influenced by substrate types. Here we investigated such dependencies by monitoring the transformation of position-specifically 13C-labelled amino acids in coastal marine sediments...
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AbstractThe plasma membrane (PM) performs a plethora of physiological processes, the coordination of which requires spatial and temporal organization into specialized domains of different sizes, stability, protein/lipid composition and overall architecture. Compartmentalization of the PM has been particularly well studied in the yeast Saccharomyces cerevisiae, where five non-overlapping domains have been described: The Membrane Compartments containing the arginine permease Can1 (MCC), the H+-ATPase...
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The purpose of this review was to evaluate the current status of reporting prospectively assessed late morbidity after curative radiotherapy in large clinical studies.
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Randomized data assessing the longitudinal quality of life (QoL) impact of SABR in the oligometastatic setting are lacking.
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The differentially expressed long noncoding RNAs (lncRNAs) and their coreralated messenger RNAs in our study revealed that lncRNAs involved in immunopathogenic mechanisms may play a crital role in the pathogenesis of autoimmune thyroid diseases. Abstract Increasing evidence has found that long noncoding RNAs (lncRNAs) and message RNAs (mRNAs) play an important role in the progress of autoimmune thyroid diseases (AITD). So, in this study, the different expressed of lncRNA and mRNA was screened...
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We constructed lncRNA‐miRNA‐mRNA network in gastric cancer and identified the key nodes based on complex network theory. Functional enrichment analysis and survival analysis of key genes in network related to key nodes were performed. The key factors in gastric cancer regulatory network can provide new insights into understanding the pathogenesis of gastric cancer and the diagnosis and treatment of gastric cancer. Abstract The oncogenesis and progression of gastric cancer are closely correlated...
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Abstract Rab11 family interacting protein 2 (Rab11‐FIP2) is a conserved protein and effector molecule for the small GTPase Rab11. By interacting with Rab11 and MYO5B, Rab11‐FIP2 regulates endosome trafficking of plasma membrane proteins, promoting cellular motility. The endosomal trafficking system in nasopharyngeal carcinoma (NPC) remains unclear. Here, an outlier analysis using the Oncomine database suggested that Rab11‐FIP2 but not Rab11 and MYO5B was overexpressed in NPC. We confirmed that the...
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Pancreatic stellate cells augment the glucose‐stimulated insulin secretion from Min6 cells, with no possible involvement of IL6‐induced PLC‐IP3 ‐dependent pathway. Abstract Pancreatic stellate cells (PSCs) secrete various factors, which can influence the β‐cell function. The identification of stellate cell infiltration into the islets in pancreatic diseases suggests possible existence of cross‐talk between these cells. To elucidate the influence of PSCs on β‐cell function, mouse PSCs were cocultured...
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Abstract Background The small‐molecule nutlin‐3 was found to be an effective therapeutic compound and p53 activator, and acts as a murine double minute 2 antagonist, although these findings need to be clinically confirmed. The essential components of the bone marrow include mesenchymal stem cells (MSCs), which play a key role in protecting, regenerating, and proliferating hematopoietic stem cells (HSCs). This feature is vital for HSC after exposure to myelotoxic anticancer agents; nevertheless,...
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Our study reported that protein tyrosine phosphatase receptor type O (PTPRO) is involved in the pathogenesis of ulcerative colitis (UC) by regulating LPS/TLR4‐mediated inflammatory response through the nuclear factor κB signaling pathway, which suggests that PTPRO may be a promising therapeutic target for UC. Besides, PTPRO can be a promising biomarker for the diagnosis of UC. Abstract Previous studies have implicated protein tyrosine phosphatase receptor type O (PTPRO) as a key regulator in...
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Large intergenic noncoding RNA regulator of reprogramming (Linc‐RoR) promoted proliferation, migration, and invasion of pancreatic cancer cells by activating the Hippo/YAP pathway. YAP might be an underlying target of Linc‐RoR and mediate EMT in pancreatic cancer (PC); thus, Linc‐RoR might be a very meaningful biomarker for PC Abstract Large intergenic noncoding RNA regulator of reprogramming (Linc‐RoR) was first identified as a regulator to increase the emergence of induced pluripotent stem...
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1. circPIP5K1A contributed to ovarian cancer progression through targeting the miR‐661/IGFBP5 axis. 2. circPIP5K1A silencing triggered downregulation of IGFBP5 by promotion miR‐66 expression. Abstract Increasing evidence demonstrates the crucial regulatory functions of circular RNAs in different cancer types. The major aim of the current study was to establish functions of circPIP5K1A during ovarian cancer. Our results showed an increased expression of circPIP5K1A in both ovarian cancers...
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LINC01116 was highly expressed in head and neck squamous cell carcinoma. LINC01116 silencing inhibited the migration and invasion capacities of the tumor cells. LINC01116 regulated the tumor function through epithelial‐mesenchymal transition process (EMT). Abstract Long noncoding RNAs (lncRNAs) are linked to tumor development and progression. The aim of this study was to determine the prognostic significance and biological role of LINC01116 in head and neck squamous cell carcinoma (HNSCC). We identified...
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Thirteen single nucleotide polymorphisms (SNPs) in seven transcription factor genes involved in cell proliferation, apoptosis, and differentiation were genotyped in the present study using allelic discrimination real‐time polymerase chain reaction. We observed five SNPs—rs2051526 (ETV6), rs6021247 (NFATC2), rs3757769 (SND1), rs7085532 (TCF7L2), and rs7778413 (SND1) indicating increased oral cancer risk with OR ranging from 1.61 to 34.60. The current study indicates the association of transcription...
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Treatment with Dex increased the viability of both cell lines; this effect continued for at least 24 hours after Dex was removed. DDP toxicity was attenuated by 10 nM Dex added either before or with DDP treatment. However, pretreatment with 50 nM Dex instead enhanced the toxicity of DDP. Abstract With the extensive use of dexmedetomidine (Dex) in the surgical resection of tumours for its potent sedative and analgesic properties, its effects on various properties of tumours have received increased...
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The effects of miR‐27a on UVB irradiation‐induced CPD formation in Hacat cells. A, CPD formation was measured by immunofluorescence (Green). DAP I (blue) was used to stain the nucleus. B, Dot blot was used to the CPD formation. Abstract MiRNAs were involved in the various biological process through mediating the posttranscriptional gene silencing. The abnormal expression of miRNAs is also involved in various disorders. Our previous study showed that miRNA‐27a (miR‐27a) was upregulated after ultraviolet...
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Abstract Osteoporosis (OP), a common metabolic bone disease, is accompanied by reduced bone mass, bone mineral density (BMD), as well as microstructure destruction of bone. Previously, microRNA‐196a‐2 (miR‐196a‐2) and miR‐196a‐3p were reported for its involvement in BMD. Herein, this study set out to identify the functional relevance of miR‐196a in osteogenic differentiation in osteoporotic mice and explore the associated mechanism by establishing an OP mouse model. Guanine nucleotide binding protein,...
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We showed that miR‐605‐5p plays a tumor‐suppressor role in non–small‐cell lung cancer (NSCLC). It functions mainly by targeting tumor necrosis factor α‐induced protein 3 (TNFAIP3) expression. These findings suggest a specific mechanism of NSCLC progression and suggest miR‐605‐5p as a potential therapeutic target for NSCLC. Abstract Lung cancer is an significant cause of death worldwide, and non–small‐cell lung cancer (NSCLC) is the most common type of lung cancer. MicroRNAs (miRNAs) have been...
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miR‐499a‐5p could relief the injury of cardiomyocytes induced by hypoxia/reoxygenation via targeting CD38. Abstract The aim is to investigate the mechanism of miR‐499a‐5p on the damage of cardiomyocyte induced by hypoxia/reoxygenation. The activity of lactate dehydrogenase (LDH), apoptosis rate and the expression of miR‐499a‐5p and cluster of differentiation 38 (CD38) in hypoxia‐reoxygenation model cells were detected by LDH Cytotoxicity Assay Kit, flow cytometry, real‐time polymerase chain...
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Abstract Kazakh sheep are seasonal estrous animals, and gonadotropin‐releasing hormone (GnRH) is the key to fertility regulation. The nutritional level has a certain regulatory effect on estrous, and vitamin B folate plays a role in DNA methylation, directly participating in the process. The goal of this study was to determine whether folate is involved in GnAQ methylation and its effect on GnRH secretion. The hypothalamic neurons of Kazakh fetal sheep were treated with folate at concentrations...
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The present study assessed the molecular mechanism of microRNA‐125a (miR‐125a) in the proliferation and apoptosis of multiple myeloma cells. The results confirmed that miR‐125a inhibited the expression of ubiquitin‐specific peptidase 5. Abstract miR‐125a is a microRNA that is frequently diminished in various human malignancies. However, the mechanism by which impaired miR‐125a promotes cancer growth remains undefined. In this study, we investigated the role of miR‐125a in the proliferation and...
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Focus on exosomes—From pathogenic mechanisms to the potential clinical application value in lymphoma
Abstract Exosomes are highly specialized and functional bilayer membranous particles. They have been considered as vehicles for transporting and delivering a large number of proteins, lipids, and nucleic acids (gene, noncoding RNA, DNA) from parental to recipient cells. In hematological malignancies, exosomes are involved in the tumorigenesis, including producing growth factors, hindering antitumor immunoreaction, promote inflammation, angiogenesis, and hypercoagulation. With the deepening of understanding,...
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Upregulated small nucleolar RNA host gene 3 (SNHG3) and serglycin (SRGN) predict poor outcome in adult acute myeloid leukemia (AML) patients. Silencing SNHG3 or SRGN results in suppressed cell growth in AML cells. SNHG3 modulates SRGN expression via sponging miR‐758‐3p. SNHG3 modulates AML cell growth through regulating the miR‐758‐3p/SRGN axis. Abstract Small nucleolar RNA host gene 3 (SNHG3) is a newly identified long non‐coding RNA whose dysregulation has been reported in several cancers....
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JDB, Vol. 7, Pages 17: Integrins Have Cell-Type-Specific Roles in the Development of Motor Neuron Connectivity Journal of Developmental Biology doi: 10.3390/jdb7030017 Authors: Devyn Oliver Emily Norman Heather Bates Rachel Avard Monika Rettler Claire Y. Bénard Michael M. Francis Michele L. Lemons Formation of the nervous system requires a complex series of events including proper extension and guidance of neuronal axons and dendrites. Here we investigate the requirement...
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Abstract Although widely used as a preclinical model for studying cardiovascular diseases, there is a scarcity of in vivo hemodynamic measurements of the naïve murine system in multiple arterial and venous locations, from head-to-toe, and across sex and age. The purpose of this study is to quantify cardiovascular hemodynamics in mice at different locations along the vascular tree while evaluating the effects of sex and age. Male and female, adult and aged mice were anesthetized...
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Abstract To evaluate the changes in bacterial diversity at various time points under resveratrol supplementation, we aimed to investigate the diversification of gut microbiota and the changes in total genetic diversity. We performed 16S rDNA gene sequencing at different time points (15, 30, and 45 days) to analyze the gut microbiota of tilapia. Fusobacteria, Proteobacteria, and Bacteroidetes (15 days) or Cyanobacteria (30 and 45 days) were found to be the three most abundant phyla....
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Abstract A novel actinomycete, strain PA1-10T, isolated from the leaf of Phyllanthus amarus collected from Bangkok, Thailand, was characterized taxonomically using a polyphasic approach. This strain contained the characteristics consistent with those of members of the genus Nonomuraea. It formed short rugose spore chain on aerial mycelium. The diamino acid in cell wall peptidoglycan was meso-diaminopimelic acid. Galactose, glucose, madurose, mannose, and ribose were found in whole-cell...
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Abstract Anaerobic digestion, a recently hot technology to produce biogases especially methane generation for biofuel from wastewater, is considered an effective explanation for energy crisis and global pollution threat. A complex microbiome population is present in sludge, which plays an important role in the digestion of complex polymer into simple monomers. 16S rRNA approaches simply are not enough for amplification due to the involvement of extreme complex population. However,...
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Abstract Introduction Treatment options for cancer metastases, the primary cause of cancer mortality, are limited. The chemokine receptor CXCR4 is an attractive therapeutic target in cancer because it mediates metastasis by inducing cancer cell and macrophage migration. Here we engineered carrier-free CXCR4-targeting RNA-protein nanoplexes that not only inhibited cellular migration but also polarized macrophages to the M1 phenotype. ...
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Abstract Purpose With the advent of innovation in the preclinical studies, micro-CT provides optimized resolution by high-power X-ray output tube and flat-panel detector with ultra-short scanning time. The aim of this study was to evaluate the performance of a novel preclinical micro-CT with different parameters and protocols. Methods Imaging quality was...
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Abstract Purpose The aim of this study is to examine how aesthetic devices are defined and managed. Regulation discrepancies between clinical use and home use in four regions are also described. Methods The official websites and databases of US, European, Chinese, and Taiwanese authorities were used to collect regulation information for aesthetic devices....
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Publication date: Available online 26 August 2019Source: Archives of Oral BiologyAuthor(s): Huan Liu, Lanxi Qin, Yue Wu, Hilbert W. van der Glas, Jianshe Chen, Xinmiao WangAbstractObjectivesWith an increasing number of elderly in China, it becomes imperative to establish a comprehensive understanding of their physiological capabilities and investigate fundamental mechanisms underpinning the discrepancies between Chinese individuals and other ethnical groups, which will facilitate government policymaking,...
19h
Checkpoint kinase 2 (CHK-2) is a key component of the DNA damage response (DDR). CHK-2 is activated by the PIP3-kinase-like kinases (PI3KKs) ataxia telangiectasia mutated (ATM) and ataxia telangiectasia and Rad3-related protein (ATR), and in metazoan also by DNA-dependent protein kinase catalytic subunit (DNA-PKcs). These DNA damage-dependent activation pathways are...
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Microsomal triglyceride transfer protein (MTP) plays an essential role in lipid metabolism, especially in the biogenesis of very low-density lipoproteins and chylomicrons via the transfer of neutral lipids and the assembly of apoB-containing lipoproteins. Our understanding of the molecular mechanisms of MTP has been hindered by a lack of structural...
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Hydrophobic base stacking is a major contributor to DNA double-helix stability. We report the discovery of specific unstacking effects in certain semihydrophobic environments. Water-miscible ethylene glycol ethers are found to modify structure, dynamics, and reactivity of DNA by mechanisms possibly related to a biologically relevant hydrophobic catalysis. Spectroscopic data and...
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Ubiquitin serves as a protein modifier and pervasive signaling molecule in eukaryotes, regulating major events throughout the lifetime of a cell, including pathways used for synthesis, repair, and degradation. Aptly named for its ubiquitous presence in human cells, proteomics studies have revealed tens of thousands of sites in ∼5,000 substrates,...
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The membranes of the first protocells on the early Earth were likely self-assembled from fatty acids. A major challenge in understanding how protocells could have arisen and withstood changes in their environment is that fatty acid membranes are unstable in solutions containing high concentrations of salt (such as would have...
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Debilitating cancer-induced muscle wasting, a syndrome known as cachexia, is lethal. Here we report a posttranscriptional pathway involving the RNA-binding protein HuR as a key player in the onset of this syndrome. Under these conditions, HuR switches its function from a promoter of muscle fiber formation to become an inducer...
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The peptide natural product nisin has been used as a food preservative for 6 decades with minimal development of resistance. Nisin contains the unusual amino acids dehydroalanine and dehydrobutyrine, which are posttranslationally installed by class I lanthipeptide dehydratases (LanBs) on a linear peptide substrate through an unusual glutamyl-tRNA–dependent dehydration of...
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Ubiquitin (Ub)-mediated proteolysis is a fundamental mechanism used by eukaryotic cells to maintain homeostasis and protein quality, and to control timing in biological processes. Two essential aspects of Ub regulation are conjugation through E1-E2-E3 enzymatic cascades and recognition by Ub-binding domains. An emerging theme in the Ub field is that...
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Second harmonic generation (SHG) is an emergent biophysical method that sensitively measures real-time conformational change of biomolecules in the presence of biological ligands and small molecules. This study describes the successful implementation of SHG as a primary screening platform to identify fragment ligands to oncogenic Kirsten rat sarcoma (KRas). KRas...
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Accurate mitosis depends on a surveillance system called the spindle assembly checkpoint. This checkpoint acts at kinetochores, which attach chromosomes to the dynamic tips of spindle microtubules. When a kinetochore is unattached or improperly attached, the protein kinase Mps1 phosphorylates kinetochore components, catalyzing the generation of a diffusible “wait” signal...
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Mutations in transcription factor p63 are associated with developmental disorders that manifest defects in stratified epithelia including the epidermis. The underlying cellular and molecular mechanism is however not yet understood. We established an epidermal commitment model using human induced pluripotent stem cells (iPSCs) and characterized differentiation defects of iPSCs derived...
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Publication date: Available online 26 August 2019Source: Advances in Biological RegulationAuthor(s): Jordy C.G. van der Zwet, Valentina Cordo, Kirsten Canté-Barrett, Jules P.P. MeijerinkAbstractIn the last decade, tremendous progress in curative treatment has been made for T-ALL patients using high-intensive, risk-adapted multi-agent chemotherapy. Further treatment intensification to improve the cure rate is not feasible as it will increase the number of toxic deaths. Hence, about 20% of pediatric...
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Publication date: Available online 26 August 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Tea Mužić, Fatma Tounsi, Søren B. Madsen, Denis Pollakowski, Manfred Konrad, Thomas HeimburgAbstractWe investigated melting transitions in native biological membranes containing their membrane proteins. The membranes originated from E. coli, B. subtilis, lung surfactant and nerve tissue from the spinal cord of several mammals. For some preparations, we studied the pressure, pH and...
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Publication date: Available online 26 August 2019Source: Bioorganic & Medicinal ChemistryAuthor(s): Sandra Codony, Elena Valverde, Rosana Leiva, José Brea, M. Isabel Loza, Christophe Morisseau, Bruce D. Hammock, Santiago VázquezAbstractSoluble epoxide hydrolase (sEH) inhibitors are potential drugs for several diseases. Adamantyl ureas are excellent sEH inhibitors but have limited metabolic stability. Herein, we report the effect of replacing the adamantane group by alternative polycyclic hydrocarbons...
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Publication date: Available online 26 August 2019Source: Bioorganic & Medicinal ChemistryAuthor(s): Nicola Porta, Julia Zaschke-Kriesche, Benedikt Frieg, Mohanraj Gopalswamy, Aleksandra Zivkovic, Manuel Etzkorn, Holger Stark, Sander H.J. Smits, Holger GohlkeAbstractLantibiotics are antimicrobial peptides produced by Gram-positive bacteria and active in the nanomolar range. Nisin is the most intensely studied and used lantibiotic, with applications as food preservative and recognized potential...
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Publication date: Available online 26 August 2019Source: Bioorganic & Medicinal ChemistryAuthor(s): David Bickel, Holger GohlkeAbstractCells constantly need to adopt to changing environmental conditions, maintaining homeostasis and proteostasis. Heat shock proteins are a diverse class of molecular chaperones that assist proteins in folding to prevent stress-induced misfolding and aggregation. The heat shock protein of 90 kDa (HSP90) is the most abundant heat shock protein. While basal expression...
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Publication date: Available online 26 August 2019Source: Free Radical Biology and MedicineAuthor(s): Natalia Sasoni, Danisa M.L. Ferrero, Sergio A. Guerrero, Alberto A. Iglesias, Diego G. AriasAbstractBackgroundGlutathione (GSH) plays a role as a main antioxidant metabolite in all eukaryotes and many prokaryotes. Most of the organisms synthesize GSH by a pathway involving two enzymatic reactions, each one consuming one molecule of ATP. In a first step mediated by glutamate-cysteine ligase (GCL),...
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Publication date: 1 November 2019Source: International Journal of Biological Macromolecules, Volume 140Author(s): Luis Fernando Gonçalves Rabelo, Bruno Antonio Ferreira, Simone Ramos Deconte, Tatiana Carla Tomiosso, Patty Karina dos Santos, Silvia Passos Andrade, Heloisa Sobreiro Selistre de Araújo, Fernanda de Assis AraújoAbstractAlternagin-C (ALT-C), a disintegrin-like protein obtained from the venom of Bothrops alternatus, is able to modulate cellular behaviors such as adhesion, migration and...
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Publication date: 1 November 2019Source: International Journal of Biological Macromolecules, Volume 140Author(s): Yan Chen, Lei Zhao, Ting He, Zhuoshen Ou, Zhuoyan Hu, Kai WangAbstractThis study investigated effects of mango peel powder on starch digestion properties and quality characteristics of bread, and discussed underneath mechanisms. Starch digestion rate and extent of bread were evaluated in vitro, and bread quality characteristics, including moisture content, volume, color and texture, were...
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Publication date: 1 November 2019Source: International Journal of Biological Macromolecules, Volume 140Author(s): Senthil Muthu Kumar Thiagamani, Senthilkumar Krishnasamy, Chandrasekar Muthukumar, Jiratti Tengsuthiwat, Rajini Nagarajan, Suchart Siengchin, Sikiru O. IsmailAbstractThis work focuses on the fabrication of hybrid bio-composites using green epoxy as the matrix material, hemp (H) and sisal (S) fibre mats as the reinforcements. The hybrid composite with sisal/hemp fibres were fabricated...
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Publication date: 1 November 2019Source: International Journal of Biological Macromolecules, Volume 140Author(s): Mohammad Zahirul Islam, Buem-Jun Park, Young-Tack LeeAbstractThis study was conducted to confirm the effects of salinity stress on bioactive compounds and antioxidant activity of wheat microgreen extract. The microgreens were cultivated for 8 days in organic media with different concentrations of Na [0 (control), 12.5, 25, 50, and 100 mM from sodium chloride] which was contained in a...
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Publication date: 1 November 2019Source: International Journal of Biological Macromolecules, Volume 140Author(s): Zhuoyue Song, Hailun Li, Jian Liang, Yingtao Xu, Lijun Zhu, Xianying Ye, Jun Wu, Wei Li, Qingping Xiong, Shijie LiAbstractThe purpose of this study was to investigate the stable effect and mechanism of sulfated polysaccharide from Undaria pinnatifida (SPUP) on atherosclerotic plaque. The results showed that atherosclerotic plaques in the ApoE−/− mice of high-fat diet model group increased...
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Publication date: 1 November 2019Source: International Journal of Biological Macromolecules, Volume 140Author(s): K. Rasheeda, D. Samyuktha, N. Nishad FathimaAbstractType I collagen is a vital structural component of the extracellular matrix providing the connective tissues with biomechanical support. One of the interesting properties of collagen is to self-associate into fibrils. The present work aims to direct the self-assembly of collagen through different molecular forces, which are tuned on...
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Publication date: 1 November 2019Source: International Journal of Biological Macromolecules, Volume 140Author(s): Siamand Ilkhanizadeh, Jabbar Khalafy, Mohammad G. DekaminAbstractA library of known and new 4-aroyl-pyrano[3,2-c]chromenes have been synthesized through the one-pot, three-component reaction of 4-hydroxycoumarin, aryl glyoxals, and malononitrile or ethyl cyanoacetate in the presence of sodium alginate without any post modification, as a biopolymeric bifunctional organocatalyst, in EtOH...
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Publication date: 1 November 2019Source: International Journal of Biological Macromolecules, Volume 140Author(s): Xiaomei Wang, Zhongshan Zhang, Hongchang Zhou, Xue Sun, Xinping Chen, Nianjun XuAbstractThe anti-aging activity of marine macroalgae Gracilaria lemaneiformis polysaccharide (GP) on Caenorhabditis elegans was evaluated by observing the lifespan, reproduction, pharyngeal pumping and stress response of worms. Moreover, quantitative fluorescence of polyglutamic acid and nuclear localization...
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Publication date: 1 November 2019Source: International Journal of Biological Macromolecules, Volume 140Author(s): A.M. Omer, R.E. Khalifa, T.M. Tamer, M. Elnouby, A.M. Hamed, Y.A. Ammar, A.A. Ali, M. Gouda, M.S. Mohy EldinAbstractA novel superoleophilic-hydrophobic nonanyl chitosan-poly (butyl acrylate) grafted copolymer was fabricated as a low-cost oil-adsorbent. Chitosan (CS) was coupled with a hydrophobic nonanal (N) to form nonanyl chitosan (NCS) schiff base, and followed by grafting with butyl...
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Publication date: 1 November 2019Source: International Journal of Biological Macromolecules, Volume 140Author(s): Alberto Ciaramella, Gianluca Catucci, Gianfranco Gilardi, Giovanna Di NardoAbstractClass VII cytochromes P450 are self-sufficient enzymes carrying a phthalate family oxygenase-like reductase domain and a P450 domain fused in a single polypeptide chain. The biocatalytic applications of CYP116B members are limited by the need of the NADPH cofactor and the lack of crystal structures as a...
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Publication date: 1 November 2019Source: International Journal of Biological Macromolecules, Volume 140Author(s): Zhengzheng Liao, Jingying Zhang, Jinyu Wang, Tingxu Yan, Fanxing Xu, Bo Wu, Feng Xiao, Kaishun Bi, Jumin Niu, Ying JiaAbstractDiabetic nephropathy (DN) with high morbidity and mortality is one of the most severe diabetes complications and affects nearly one-third of people with diabetes. Our present experiment was designed to assess the potential therapeutic of a polysaccharide purified...
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Publication date: 1 November 2019Source: International Journal of Biological Macromolecules, Volume 140Author(s): Carolina Petri Bernardes, Danilo Luccas Menaldo, Karina Furlani Zoccal, Johara Boldrini-França, Steve Peigneur, Eliane Candiane Arantes, José Cesar Rosa, Lúcia Helena Faccioli, Jan Tytgat, Suely Vilela SampaioAbstractCRISPs represent a family of cysteine-rich secretory proteins with molecular mass between 20 and 30 kDa and a highly conserved specific pattern of 16 cysteine residues. In...
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Publication date: 1 November 2019Source: International Journal of Biological Macromolecules, Volume 140Author(s): Maria Bercea, Bernhard A. WolfAbstractThe viscometric behaviour of κ-carrageenan in aqueous solutions and in the presence of monovalent salts was investigated at 25 °C. Coil, helix or double helix conformations were induced by cooling hot κ-carrageenan solutions under appropriate ionic conditions. A new viscometric approach was used for modeling the behaviour of κ-carrageenan solutions....
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Publication date: 1 November 2019Source: International Journal of Biological Macromolecules, Volume 140Author(s): Xiao Chen, Lan Tao, Yi Ru, Shaohuang Weng, Zhou Chen, Jian Wang, Longhua Guo, Zhenyu Lin, Wei Pan, Bin QiuAbstractTetrastigma hemsleyanum Diels et Gilg (THDG) is used as a Chinese traditional anti-inflammatory medicine for about thousands of years. In this work, Tetrastigma hemsleyanum Diels et Gilg's polysaccharide (TP) can inhibit E. coli's growth in initial dosing period. Compared...
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Publication date: 1 November 2019Source: International Journal of Biological Macromolecules, Volume 140Author(s): Cynthia Li, Tsutomu ArakawaAbstractWe have developed an agarose-based native gel electrophoresis system that works for both acidic and basic proteins using histidine-MES buffer. This electrophoresis can be done in a flat-bed mode or a vertical mode. While in the flat-bed mode both acidic and basic proteins can be simultaneously analyzed, the vertical gel can only be used for either protein....
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Publication date: Available online 26 August 2019Source: New BiotechnologyAuthor(s): Sílvia A. Sousa, Pedro Soares-Castro, António M.M. Seixas, Joana R. Feliciano, Bernardo Balugas, Celeste Barreto, Luísa Pereira, Pedro M. Santos, Jorge H. LeitãoAbstractBacteria of the Burkholderia cepacia complex (Bcc) are ubiquitous multidrug resistant organisms and opportunistic pathogens capable of causing life threatening lung infections among cystic fibrosis (CF) patients. No effective therapies are currently...
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Publication date: Available online 26 August 2019Source: Respiratory Physiology & NeurobiologyAuthor(s): Zhongcui Yu, Yubo Jiang, Congling SunAbstractBackgroundThe purpose of this study was to explore the effect of Wnt pathway on the inhibition of airway epithelial cells repair by glucocorticoid.Materials and MethodsThe expression of E-cadherin in asthma mice model was detected by immunocytochemistry. XAV939 was used to treat 16HBE, and the expressions of related genes were determined by western...
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Publication date: Available online 26 August 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): AD van den Brand, E Rubinstein, PC de Jong, M van den Berg, MBM van DuursenAbstractHuman and rat reproductive systems differ significantly with respect to hormonal cyclicity and endometrial cell behavior. However, species-differences in endometrial cell responses upon hormonal stimulation and exposure to potentially toxic compounds are poorly characterized. In this study, human...
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Publication date: Available online 26 August 2019Source: Ultrasound in Medicine & BiologyAuthor(s): Ernest L. Madsen, Chihwa Song, Gary R. Frank
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