Κυριακή 11 Αυγούστου 2019


D‐dimer levels and risk of recurrence following provoked venous thromboembolism: Findings from the RIETE registry
Abstract Background Patients with venous thromboembolism (VTE) secondary to transient risk factors may develop VTE recurrences after discontinuing anticoagulation. Identifying at‐risk patients could help to guide the duration of therapy. Methods We used the RIETE database to assess the prognostic value of d‐dimer testing after discontinuing anticoagulation to identify patients at increased risk for recurrences. Transient risk factors were classified as major (postoperative) or...
Journal of Internal Medicine
2d
Investigating Long-term Synaptic Plasticity in Interlamellar Hippocampus CA1 by Electrophysiological Field Recording
We used recording and stimulation electrodes in longitudinal hippocampal brain slices and longitudinally positioned recording and stimulation electrodes in the dorsal hippocampus in vivo to evoke extracellular postsynaptic potentials and demonstrate long-term synaptic plasticity along the longitudinal interlamellar CA1.
JoVE: Journal of Visualized Experiments
11h
Hybrid Microdrive System with Recoverable Opto-Silicon Probe and Tetrode for Dual-Site High Density Recording in Freely Moving Mice
This protocol describes the construction of a hybrid microdrive array that allows implantation of nine independently adjustable tetrodes and one adjustable opto-silicon probe in two brain regions in freely moving mice. Also demonstrated is a method for safely recovering and reusing the opto-silicon probe for multiple purposes.
JoVE: Journal of Visualized Experiments
1d
Targeted Studies Using Serial Block Face and Focused Ion Beam Scan Electron Microscopy
Here, we present a protocol for efficiently combining serial block face and focused ion beam scanning electron microscopy to target an area of interest. This allows for efficient searching, in three dimensions, and locating rare events in a large field of view.
JoVE: Journal of Visualized Experiments
1d
Isolation of Mouse Epidermal Keratinocytes and Their In Vitro Clonogenic Culture
The goal of this protocol is to isolate epidermal keratinocytes from the dorsal skin of adult mice for a variety of downstream applications such as molecular biology, biochemistry, fluorescence activated cell sorting, and primary in vitro uses (e.g., clonogenic keratinocytes).
JoVE: Journal of Visualized Experiments
1d
Evaluation of Photosynthetic Behaviors by Simultaneous Measurements of Leaf Reflectance and Chlorophyll Fluorescence Analyses
We describe a new technical approach to study photosynthetic responses in higher plants involving simultaneous measurements of chlorophyll a fluorescence and leaf reflectance using a PAM and a spectral radiometer for the detection of signals from the same leaf area in Arabidopsis.
JoVE: Journal of Visualized Experiments
2d
Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells
Here, we present a protocol for immunophenotypic characterization and cytokine induced differentiation of cord blood derived CD34+ hematopoietic stem and progenitor cells to the four myeloid lineages. The applications of this protocol include investigations on the effect of myeloid disease mutations or small molecules on myeloid differentiation of the CD34+ cells.
JoVE: Journal of Visualized Experiments
2d
Cultivation of the Marine Pelagic Tunicate Dolioletta gegenbauri (Uljanin 1884) for Experimental Studies
Doliolids, including the species Dolioletta gegenbauri, are small gelatinous marine zooplankton of ecological significance found on productive subcontinental shelf systems worldwide. The difficulty of culturing these delicate organisms limits their investigation. In this study, we describe cultivation approaches for collecting, rearing, and maintaining the doliolid Dolioletta gegenbauri.
JoVE: Journal of Visualized Experiments
2d
Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
Sequence specificity is critical for gene regulation. Regulatory proteins that recognize specific sequences are important for gene regulation. Defining functional binding sites for such proteins is a challenging biological problem. An iterative approach for identification of a binding site for an RNA-binding protein is described here and is applicable to all RNA-binding proteins.
JoVE: Journal of Visualized Experiments
2d
Visualizing Lymph Node Structure and Cellular Localization using Ex-Vivo Confocal Microscopy
This protocol describes a technique to image different cell populations in draining lymph nodes without alterations in the organ structure.
JoVE: Journal of Visualized Experiments
2d
Highly Sensitive Measurement of Glomerular Permeability in Mice with Fluorescein Isothiocyanate-polysucrose 70
Here, we present a protocol to test glomerular permeability in mice using a highly sensitive, nonradioactive tracer. This method allows repetitive urine analyses with small urine volumes.
JoVE: Journal of Visualized Experiments
2d
A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants
This article describes a novel method to generate tumor antigen-specific induced pluripotent stem cell-derived thymic emigrants (iTE) by a three-dimensional (3D) thymic culture system. iTE are a homogenous subset of T cells closely related to naïve T cells with the capacity for proliferation, memory formation, and tumor suppression.
JoVE: Journal of Visualized Experiments
2d
Inguinal microbiome in patients undergoing an endovascular aneurysm repair: Application of next-generation sequencing of the 16S-23S rRNA regions
Publication date: Available online 10 August 2019Source: Medical HypothesesAuthor(s): B.P. Vierhout, A. Ott, I. Kruithof, G. Wisselink, E. van Zanten, A.M.D. Kooistra-Smid, C.J. Zeebregts, R.A. PolAbstractBackgroundSurgical site infection (SSI) remains a hazardous complication after vascular surgery. In this pilot study we investigated the inguinal microbiome in skin biopsies using histology and 16S-23S rDNA Next Generation Sequencing (NGS). Our hypothesis was that causative microorganisms of SSI...
ScienceDirect Publication: Medical Hypotheses
14h
Toward a cure for lumbar spinal stenosis: The potential of interspinous process decompression
Publication date: Available online 10 August 2019Source: Medical HypothesesAuthor(s): Jon E. Block, William F. Lavelle, Pierce D. NunleyAbstractThere is a growing impetus to treat aging as a disease in the quest to significantly extend the human life span through cellular regeneration methods. This approach, while promising, overlooks the fact that the evolutionary adaptation to bipedalism puts the human body in a distinctively vulnerable biomechanical and functional position. Orthograde human posture...
ScienceDirect Publication: Medical Hypotheses
14h

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